Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles.
Deep level insect relationships are generally difficult to resolve, especially within taxa of the most diverse and species rich holometabolous orders. In beetles, the major diversity occurs in the Phytophaga, including charismatic groups such as leaf beetles, longhorn beetles and weevils. Bark and a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5036811?pdf=render |
| _version_ | 1818892187402764288 |
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| author | Dario Pistone Sigrid Mugu Bjarte Henry Jordal |
| author_facet | Dario Pistone Sigrid Mugu Bjarte Henry Jordal |
| author_sort | Dario Pistone |
| collection | DOAJ |
| description | Deep level insect relationships are generally difficult to resolve, especially within taxa of the most diverse and species rich holometabolous orders. In beetles, the major diversity occurs in the Phytophaga, including charismatic groups such as leaf beetles, longhorn beetles and weevils. Bark and ambrosia beetles are wood boring weevils that contribute 12 percent of the diversity encountered in Curculionidae, one of the largest families of beetles with more than 50000 described species. Phylogenetic resolution in groups of Cretaceous age has proven particularly difficult and requires large quantity of data. In this study, we investigated 100 nuclear genes in order to select a number of markers with low evolutionary rates and high phylogenetic signal. A PCR screening using degenerate primers was applied to 26 different weevil species. We obtained sequences from 57 of the 100 targeted genes. Sequences from each nuclear marker were aligned and examined for detecting multiple copies, pseudogenes and introns. Phylogenetic informativeness (PI) and the capacity for reconstruction of previously established phylogenetic relationships were used as proxies for selecting a subset of the 57 amplified genes. Finally, we selected 16 markers suitable for large-scale phylogenetics of Scolytinae and related weevil taxa. |
| first_indexed | 2024-12-19T17:52:43Z |
| format | Article |
| id | doaj.art-c963b1846b7741608b86c65551777341 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T17:52:43Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c963b1846b7741608b86c655517773412022-12-21T20:11:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01119e016352910.1371/journal.pone.0163529Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles.Dario PistoneSigrid MuguBjarte Henry JordalDeep level insect relationships are generally difficult to resolve, especially within taxa of the most diverse and species rich holometabolous orders. In beetles, the major diversity occurs in the Phytophaga, including charismatic groups such as leaf beetles, longhorn beetles and weevils. Bark and ambrosia beetles are wood boring weevils that contribute 12 percent of the diversity encountered in Curculionidae, one of the largest families of beetles with more than 50000 described species. Phylogenetic resolution in groups of Cretaceous age has proven particularly difficult and requires large quantity of data. In this study, we investigated 100 nuclear genes in order to select a number of markers with low evolutionary rates and high phylogenetic signal. A PCR screening using degenerate primers was applied to 26 different weevil species. We obtained sequences from 57 of the 100 targeted genes. Sequences from each nuclear marker were aligned and examined for detecting multiple copies, pseudogenes and introns. Phylogenetic informativeness (PI) and the capacity for reconstruction of previously established phylogenetic relationships were used as proxies for selecting a subset of the 57 amplified genes. Finally, we selected 16 markers suitable for large-scale phylogenetics of Scolytinae and related weevil taxa.http://europepmc.org/articles/PMC5036811?pdf=render |
| spellingShingle | Dario Pistone Sigrid Mugu Bjarte Henry Jordal Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles. PLoS ONE |
| title | Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles. |
| title_full | Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles. |
| title_fullStr | Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles. |
| title_full_unstemmed | Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles. |
| title_short | Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles. |
| title_sort | genomic mining of phylogenetically informative nuclear markers in bark and ambrosia beetles |
| url | http://europepmc.org/articles/PMC5036811?pdf=render |
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