Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety
Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the pheno...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/2/178 |
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| author | Vanessa Silva Rupesh Kumar Singh Nelson Gomes Bruno Gonçalves Soares Adriana Silva Virgílio Falco Rosa Capita Carlos Alonso-Calleja José Eduardo Pereira Joana S. Amaral Gilberto Igrejas Patrícia Poeta |
| author_facet | Vanessa Silva Rupesh Kumar Singh Nelson Gomes Bruno Gonçalves Soares Adriana Silva Virgílio Falco Rosa Capita Carlos Alonso-Calleja José Eduardo Pereira Joana S. Amaral Gilberto Igrejas Patrícia Poeta |
| author_sort | Vanessa Silva |
| collection | DOAJ |
| description | Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic composition, antioxidant potential and antibacterial activity of its individual grape components. Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles, and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility method was performed using the Kirby-Bauer disc diffusion method against multidrug-resistant bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed the highest antioxidant and antimicrobial activities. The studied individual components obtained from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and pharmaceutical industries as natural food preservers and antibiotic adjuvants. |
| first_indexed | 2024-03-12T10:08:58Z |
| format | Article |
| id | doaj.art-c964fedb8cb14c9b83c0a992131c8d77 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T10:08:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-c964fedb8cb14c9b83c0a992131c8d772023-09-02T11:03:10ZengMDPI AGAntioxidants2076-39212020-02-019217810.3390/antiox9020178antiox9020178Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão VarietyVanessa Silva0Rupesh Kumar Singh1Nelson Gomes2Bruno Gonçalves Soares3Adriana Silva4Virgílio Falco5Rosa Capita6Carlos Alonso-Calleja7José Eduardo Pereira8Joana S. Amaral9Gilberto Igrejas10Patrícia Poeta11Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentro de Química-Vila Real (CQ-VR), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalDepartment of Genetics and Biotechnology, Functional Genomics and Proteomics’ Unit, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalCoLAB Vines&Wines—National Collaborative Laboratory for the Portuguese Wine Sector, Associação para o Desenvolvimento da Viticultura Duriense (ADVID), 5000-801 Vila Real, PortugalMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentro de Química-Vila Real (CQ-VR), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalCentro de Investigação de Montanha (CIMO), Polytechnic Institute of Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalDepartment of Genetics and Biotechnology, Functional Genomics and Proteomics’ Unit, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalChitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic composition, antioxidant potential and antibacterial activity of its individual grape components. Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles, and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility method was performed using the Kirby-Bauer disc diffusion method against multidrug-resistant bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed the highest antioxidant and antimicrobial activities. The studied individual components obtained from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and pharmaceutical industries as natural food preservers and antibiotic adjuvants.https://www.mdpi.com/2076-3921/9/2/178chitosanchitosan nanoparticlesgrapevinesby-productsantioxidantantimicrobialphenolics |
| spellingShingle | Vanessa Silva Rupesh Kumar Singh Nelson Gomes Bruno Gonçalves Soares Adriana Silva Virgílio Falco Rosa Capita Carlos Alonso-Calleja José Eduardo Pereira Joana S. Amaral Gilberto Igrejas Patrícia Poeta Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety Antioxidants chitosan chitosan nanoparticles grapevines by-products antioxidant antimicrobial phenolics |
| title | Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety |
| title_full | Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety |
| title_fullStr | Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety |
| title_full_unstemmed | Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety |
| title_short | Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety |
| title_sort | comparative insight upon chitosan solution and chitosan nanoparticles application on the phenolic content antioxidant and antimicrobial activities of individual grape components of sousao variety |
| topic | chitosan chitosan nanoparticles grapevines by-products antioxidant antimicrobial phenolics |
| url | https://www.mdpi.com/2076-3921/9/2/178 |
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