Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application
This research evaluated the antifungal effectiveness of <i>Arthrospira platensis</i> ethanol, methanol, ethyl acetate and acetone extracts against the tested pathogenic fungi (<i>Candida albicans</i>, <i>Trichophyton rubrum</i> and <i>Malassezia furfur</i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/3/160 |
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| author | Saly Gheda Eman H. F. Abd El-Zaher Alaa M. Abou-Zeid Nesma A. Bedair Leonel Pereira |
| author_facet | Saly Gheda Eman H. F. Abd El-Zaher Alaa M. Abou-Zeid Nesma A. Bedair Leonel Pereira |
| author_sort | Saly Gheda |
| collection | DOAJ |
| description | This research evaluated the antifungal effectiveness of <i>Arthrospira platensis</i> ethanol, methanol, ethyl acetate and acetone extracts against the tested pathogenic fungi (<i>Candida albicans</i>, <i>Trichophyton rubrum</i> and <i>Malassezia furfur</i>). Antioxidant and cytotoxicity effectiveness of <i>A. platensis</i> extracts against four distinct cell lines were also assessed. Methanol extract of <i>A. platensis</i> exhibited the highest inhibition zones against <i>Candida albicans</i> as measured by the well diffusion method. A transmission electron micrograph of the treated group of <i>Candida</i> cells with <i>A. platensis</i> methanolic extract showed mild lysis and vacuolation of the cytoplasmic organelles. In vivo, after induced infection of mice by <i>C. albicans</i> and treatment with <i>A. platensis</i> methanolic extract cream, the skin layer emerged with the removal of <i>Candida</i> spherical plastopores. The extract of <i>A. platensis</i> recorded the highest antioxidant activity using the DPPH (2, 2- diphenyl-1-picrylhydrazyl) scavenging method (IC<sub>50</sub> 28 mg/mL). A cytotoxicity test using a MTT assay showed that the <i>A. platensis</i> extract had strong cytotoxic activity against the HepG2 cell line (IC<sub>50</sub> 20.56 ± 1.7 μg/mL) and moderate cytotoxic activity against MCF7 and the Hela cell (IC<sub>50</sub> 27.99 ± 2.1 μg/mL). Gas Chromatography/Mass Spectroscopy (GC/MS) results revealed that the effective activity of <i>A. platensis</i> extract could be linked to a synergistic impact between their prominent composition as alkaloids, phytol, fatty acids hydrocarbons, phenolics and phthalates. <i>A. platensis</i> extract contains active metabolites that constitute a promising source of antifungal, antioxidant and anti-proliferative compounds for the pharmaceutical drug industry. |
| first_indexed | 2024-03-11T06:16:38Z |
| format | Article |
| id | doaj.art-c96b4d03a6884a62a4ccf5c1ad32aea8 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T06:16:38Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-c96b4d03a6884a62a4ccf5c1ad32aea82023-11-17T12:17:22ZengMDPI AGMarine Drugs1660-33972023-02-0121316010.3390/md21030160Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream ApplicationSaly Gheda0Eman H. F. Abd El-Zaher1Alaa M. Abou-Zeid2Nesma A. Bedair3Leonel Pereira4Botany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptBotany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptBotany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptBotany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptMARE—Marine and Environmental Sciences Centre, ARNET, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, PortugalThis research evaluated the antifungal effectiveness of <i>Arthrospira platensis</i> ethanol, methanol, ethyl acetate and acetone extracts against the tested pathogenic fungi (<i>Candida albicans</i>, <i>Trichophyton rubrum</i> and <i>Malassezia furfur</i>). Antioxidant and cytotoxicity effectiveness of <i>A. platensis</i> extracts against four distinct cell lines were also assessed. Methanol extract of <i>A. platensis</i> exhibited the highest inhibition zones against <i>Candida albicans</i> as measured by the well diffusion method. A transmission electron micrograph of the treated group of <i>Candida</i> cells with <i>A. platensis</i> methanolic extract showed mild lysis and vacuolation of the cytoplasmic organelles. In vivo, after induced infection of mice by <i>C. albicans</i> and treatment with <i>A. platensis</i> methanolic extract cream, the skin layer emerged with the removal of <i>Candida</i> spherical plastopores. The extract of <i>A. platensis</i> recorded the highest antioxidant activity using the DPPH (2, 2- diphenyl-1-picrylhydrazyl) scavenging method (IC<sub>50</sub> 28 mg/mL). A cytotoxicity test using a MTT assay showed that the <i>A. platensis</i> extract had strong cytotoxic activity against the HepG2 cell line (IC<sub>50</sub> 20.56 ± 1.7 μg/mL) and moderate cytotoxic activity against MCF7 and the Hela cell (IC<sub>50</sub> 27.99 ± 2.1 μg/mL). Gas Chromatography/Mass Spectroscopy (GC/MS) results revealed that the effective activity of <i>A. platensis</i> extract could be linked to a synergistic impact between their prominent composition as alkaloids, phytol, fatty acids hydrocarbons, phenolics and phthalates. <i>A. platensis</i> extract contains active metabolites that constitute a promising source of antifungal, antioxidant and anti-proliferative compounds for the pharmaceutical drug industry.https://www.mdpi.com/1660-3397/21/3/160cyanobacteriaantifungaldermatophyteantioxidantcytotoxicity |
| spellingShingle | Saly Gheda Eman H. F. Abd El-Zaher Alaa M. Abou-Zeid Nesma A. Bedair Leonel Pereira Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application Marine Drugs cyanobacteria antifungal dermatophyte antioxidant cytotoxicity |
| title | Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application |
| title_full | Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application |
| title_fullStr | Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application |
| title_full_unstemmed | Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application |
| title_short | Potential Activity of <i>Arthrospira platensis</i> as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application |
| title_sort | potential activity of i arthrospira platensis i as antioxidant cytotoxic and antifungal against some skin diseases topical cream application |
| topic | cyanobacteria antifungal dermatophyte antioxidant cytotoxicity |
| url | https://www.mdpi.com/1660-3397/21/3/160 |
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