FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway
Objective To determine whether four-and-a-half LIM-only protein 2(FHL2) can affect macrophage foaming by regulating nuclear factor kappa-B(NF-κB) signaling pathway. Methods FHL2 over-expression plasmids and siRNA of FHL2 were constructed and transfected into human monocyte/macrophages cell line THP-...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2024-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-2-204.pdf |
| _version_ | 1797345651633160192 |
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| author | CHEN Weiwei, LIAO Huang, SHI Zhenhong, LUO Ying |
| author_facet | CHEN Weiwei, LIAO Huang, SHI Zhenhong, LUO Ying |
| author_sort | CHEN Weiwei, LIAO Huang, SHI Zhenhong, LUO Ying |
| collection | DOAJ |
| description | Objective To determine whether four-and-a-half LIM-only protein 2(FHL2) can affect macrophage foaming by regulating nuclear factor kappa-B(NF-κB) signaling pathway. Methods FHL2 over-expression plasmids and siRNA of FHL2 were constructed and transfected into human monocyte/macrophages cell line THP-1. Western blot was used to detect the expression of FHL2. The cells were stimulated with oxidized low density lipoprotein(ox-LDL) and the expression of IL-6, IL-1β,TNF-α and other cytokines were detected by ELISA. Oil red O staining was used to detect the degree of cell foaming. The protein expression of NF-κB signaling pathway was detected by Western blot. Results The expression of FHL2 increased after transfected with FHL2 over-expression plasmids while decreased in si-FLH2 transfected cells. FHL2 down-regulated secretion of inflammatory cytokines. Down-regulation of FHL2 alleviated THP-1 macrophage foaming. The down-regulation of FHL2 inhibited activation of NF-κB signaling pathway, while the over-expression FHL2 showed an opposite trend. Conclusions FHL2 down-regulation inhibits the activation of NF-κB signaling pathway, reduces the secretion of inflammatory cytokines and alleviates foaming of macrophages. |
| first_indexed | 2024-03-08T11:21:50Z |
| format | Article |
| id | doaj.art-c96eaded39504dbe88e20af8608d8ec3 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T11:21:50Z |
| publishDate | 2024-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-c96eaded39504dbe88e20af8608d8ec32024-01-26T09:27:35ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252024-02-0144220420910.16352/j.issn.1001-6325.2024.02.0204FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathwayCHEN Weiwei, LIAO Huang, SHI Zhenhong, LUO Ying0Department of Cardiovascular Medicine, the Second Affiliated Hospital of Hainan Medical College, Haikou 570216,ChinaObjective To determine whether four-and-a-half LIM-only protein 2(FHL2) can affect macrophage foaming by regulating nuclear factor kappa-B(NF-κB) signaling pathway. Methods FHL2 over-expression plasmids and siRNA of FHL2 were constructed and transfected into human monocyte/macrophages cell line THP-1. Western blot was used to detect the expression of FHL2. The cells were stimulated with oxidized low density lipoprotein(ox-LDL) and the expression of IL-6, IL-1β,TNF-α and other cytokines were detected by ELISA. Oil red O staining was used to detect the degree of cell foaming. The protein expression of NF-κB signaling pathway was detected by Western blot. Results The expression of FHL2 increased after transfected with FHL2 over-expression plasmids while decreased in si-FLH2 transfected cells. FHL2 down-regulated secretion of inflammatory cytokines. Down-regulation of FHL2 alleviated THP-1 macrophage foaming. The down-regulation of FHL2 inhibited activation of NF-κB signaling pathway, while the over-expression FHL2 showed an opposite trend. Conclusions FHL2 down-regulation inhibits the activation of NF-κB signaling pathway, reduces the secretion of inflammatory cytokines and alleviates foaming of macrophages.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-2-204.pdffour-and-a-half lim-only protein 2(fhl2 )|nf-κb signaling pathway|thp-1|macrophage foaming |
| spellingShingle | CHEN Weiwei, LIAO Huang, SHI Zhenhong, LUO Ying FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway Jichu yixue yu linchuang four-and-a-half lim-only protein 2(fhl2 )|nf-κb signaling pathway|thp-1|macrophage foaming |
| title | FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway |
| title_full | FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway |
| title_fullStr | FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway |
| title_full_unstemmed | FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway |
| title_short | FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway |
| title_sort | fhl2 regulates thp 1 macrophage foaming through nf κb signaling pathway |
| topic | four-and-a-half lim-only protein 2(fhl2 )|nf-κb signaling pathway|thp-1|macrophage foaming |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-2-204.pdf |
| work_keys_str_mv | AT chenweiweiliaohuangshizhenhongluoying fhl2regulatesthp1macrophagefoamingthroughnfkbsignalingpathway |