Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers
<i>Vibrio parahaemolyticus</i> is a human foodborne pathogen, and it can form a mature biofilm on food and food contact surfaces to enhance their resistance to antibacterial agents. In this study, the effect of anti-biofilm enzymes (combined lipase, cellulase and proteinase K) on the inh...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/9/1305 |
| _version_ | 1827672525257048064 |
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| author | Yuan Li Ruyue Dong Lei Ma Yilin Qian Zunying Liu |
| author_facet | Yuan Li Ruyue Dong Lei Ma Yilin Qian Zunying Liu |
| author_sort | Yuan Li |
| collection | DOAJ |
| description | <i>Vibrio parahaemolyticus</i> is a human foodborne pathogen, and it can form a mature biofilm on food and food contact surfaces to enhance their resistance to antibacterial agents. In this study, the effect of anti-biofilm enzymes (combined lipase, cellulase and proteinase K) on the inhibition and eradication of pathogen biofilm was evaluated. The biofilm content of <i>V. parahaemolyticus</i> showed the highest level at the incubation time of 24 h, and the combined enzymes significantly inhibited the biofilm’s development. The biofilm’s inhibition and eradication rate at an incubation time of 24 h was 89.7% and 66.9%, respectively. The confocal laser scanning microscopic images confirmed that the microcolonies’ aggregation and the adhesion of biofilm were inhibited with the combined enzyme treatment. Furthermore, combined enzymes also decreased the concentration of exopolysaccharide (EPS) and disrupted the EPS matrix network, wherein the expression of the EPS-related gene, <i>cpsA-J</i>, was likewise suppressed. The combined enzymes showed an excellent inhibition effect of <i>V. parahaemolyticus</i> biofilm on different carriers, with the highest inhibition rate of 59.35% on nonrust steel plate. This study demonstrates that the combined enzyme of lipase, cellulase and proteinase K could be a novel candidate to overcome biofilm’s problem of foodborne pathogens in the food industry. |
| first_indexed | 2024-03-10T04:10:30Z |
| format | Article |
| id | doaj.art-c96f7f1d442b48969bddd4a52981f5f7 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:10:30Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-c96f7f1d442b48969bddd4a52981f5f72023-11-23T08:13:35ZengMDPI AGFoods2304-81582022-04-01119130510.3390/foods11091305Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different CarriersYuan Li0Ruyue Dong1Lei Ma2Yilin Qian3Zunying Liu4College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China<i>Vibrio parahaemolyticus</i> is a human foodborne pathogen, and it can form a mature biofilm on food and food contact surfaces to enhance their resistance to antibacterial agents. In this study, the effect of anti-biofilm enzymes (combined lipase, cellulase and proteinase K) on the inhibition and eradication of pathogen biofilm was evaluated. The biofilm content of <i>V. parahaemolyticus</i> showed the highest level at the incubation time of 24 h, and the combined enzymes significantly inhibited the biofilm’s development. The biofilm’s inhibition and eradication rate at an incubation time of 24 h was 89.7% and 66.9%, respectively. The confocal laser scanning microscopic images confirmed that the microcolonies’ aggregation and the adhesion of biofilm were inhibited with the combined enzyme treatment. Furthermore, combined enzymes also decreased the concentration of exopolysaccharide (EPS) and disrupted the EPS matrix network, wherein the expression of the EPS-related gene, <i>cpsA-J</i>, was likewise suppressed. The combined enzymes showed an excellent inhibition effect of <i>V. parahaemolyticus</i> biofilm on different carriers, with the highest inhibition rate of 59.35% on nonrust steel plate. This study demonstrates that the combined enzyme of lipase, cellulase and proteinase K could be a novel candidate to overcome biofilm’s problem of foodborne pathogens in the food industry.https://www.mdpi.com/2304-8158/11/9/1305anti-biofilm enzymes<i>Vibrio parahaemolyticus</i>biofilmexopolysaccharide |
| spellingShingle | Yuan Li Ruyue Dong Lei Ma Yilin Qian Zunying Liu Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers Foods anti-biofilm enzymes <i>Vibrio parahaemolyticus</i> biofilm exopolysaccharide |
| title | Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers |
| title_full | Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers |
| title_fullStr | Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers |
| title_full_unstemmed | Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers |
| title_short | Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of <i>Vibrio parahaemolyticus</i> Biofilm: Mechanism on <i>cpsA-J</i> Expression and Application on Different Carriers |
| title_sort | combined anti biofilm enzymes strengthen the eradicate effect of i vibrio parahaemolyticus i biofilm mechanism on i cpsa j i expression and application on different carriers |
| topic | anti-biofilm enzymes <i>Vibrio parahaemolyticus</i> biofilm exopolysaccharide |
| url | https://www.mdpi.com/2304-8158/11/9/1305 |
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