A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues
This study is pointed out to estimate the effectiveness of two solvents in the extraction and evaluating the active ingredients and their antioxidant activity as well as anti-cancer efficiency. Therefore, residues from four different Brassica vegetables viz. broccoli, Brussels sprout, cauliflower, a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
College of Science for Women, University of Baghdad
2020-07-01
|
| Series: | Baghdad Science Journal |
| Subjects: | |
| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3822 |
| _version_ | 1819054671110602752 |
|---|---|
| author | Alaa A. Gaafar Zeinab A. Salama Farouk K. El-Baz |
| author_facet | Alaa A. Gaafar Zeinab A. Salama Farouk K. El-Baz |
| author_sort | Alaa A. Gaafar |
| collection | DOAJ |
| description | This study is pointed out to estimate the effectiveness of two solvents in the extraction and evaluating the active ingredients and their antioxidant activity as well as anti-cancer efficiency. Therefore, residues from four different Brassica vegetables viz. broccoli, Brussels sprout, cauliflower, and red cherry radish were extracted using two procedures methods: methanolic and water crude extracts. Methanol extracts showed the highest content of total phenolic (TP), total flavonoids (TF), and total tannins (TT) for broccoli and Brussels sprouts residues. Methanolic extract of broccoli and Brussels sprouts residues showed the highest DPPH· scavenging activity (IC50 = 15.39 and 18.64 µg/ml). The methanol and water extracts of Brussels sprout residues showed the highest chelating activity (IC50 = 11.77 and 5.94 µg/ml) and exhibited the highest reducing power (EC50 =14.38 and 20.18 µg/ml) with broccoli respectively. The HPLC analysis of phenolic compounds confirmed that the methanol extract of all the residues examined possessed high amounts of catchine, rutin, cumaric, benzoic, and luteolin. The methanol extract at 100 µg/ml of Brussels sprouts residues displayed a rise cytotoxic effect on HePG2 (80.40%), MCF7 (75.49%) and HCT116 (22.74%) followed by broccoli and red cherry radish, respectively. This result confirmed that Brussels sprouts residue contain effective chemical compounds that can inhibit the proliferation of cancer cells. Therefore, these results proposed that those Brassica vegetable residues might be beneficial as a potent antioxidant and anticancer agents and strongly recommended as fixing in constituent's food applications and pharmaceutical industries. |
| first_indexed | 2024-12-21T12:55:19Z |
| format | Article |
| id | doaj.art-c9751a4c453742698854e3b0884cd6ac |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-12-21T12:55:19Z |
| publishDate | 2020-07-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-c9751a4c453742698854e3b0884cd6ac2022-12-21T19:03:22ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862020-07-0117310.21123/bsj.2020.17.3.0743A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable ResiduesAlaa A. Gaafar0Zeinab A. Salama1Farouk K. El-Baz2Plant Biochemistry Department, National Research Centre, Dokki, GizaPlant Biochemistry Department, National Research Centre, Dokki, Giza, Egypt.Plant Biochemistry Department, National Research Centre, Dokki, Giza, Egypt.This study is pointed out to estimate the effectiveness of two solvents in the extraction and evaluating the active ingredients and their antioxidant activity as well as anti-cancer efficiency. Therefore, residues from four different Brassica vegetables viz. broccoli, Brussels sprout, cauliflower, and red cherry radish were extracted using two procedures methods: methanolic and water crude extracts. Methanol extracts showed the highest content of total phenolic (TP), total flavonoids (TF), and total tannins (TT) for broccoli and Brussels sprouts residues. Methanolic extract of broccoli and Brussels sprouts residues showed the highest DPPH· scavenging activity (IC50 = 15.39 and 18.64 µg/ml). The methanol and water extracts of Brussels sprout residues showed the highest chelating activity (IC50 = 11.77 and 5.94 µg/ml) and exhibited the highest reducing power (EC50 =14.38 and 20.18 µg/ml) with broccoli respectively. The HPLC analysis of phenolic compounds confirmed that the methanol extract of all the residues examined possessed high amounts of catchine, rutin, cumaric, benzoic, and luteolin. The methanol extract at 100 µg/ml of Brussels sprouts residues displayed a rise cytotoxic effect on HePG2 (80.40%), MCF7 (75.49%) and HCT116 (22.74%) followed by broccoli and red cherry radish, respectively. This result confirmed that Brussels sprouts residue contain effective chemical compounds that can inhibit the proliferation of cancer cells. Therefore, these results proposed that those Brassica vegetable residues might be beneficial as a potent antioxidant and anticancer agents and strongly recommended as fixing in constituent's food applications and pharmaceutical industries.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3822Antioxidant, Brassica vegetable residues, Cytotoxic effect, HPLC, Phenolics |
| spellingShingle | Alaa A. Gaafar Zeinab A. Salama Farouk K. El-Baz A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues Baghdad Science Journal Antioxidant, Brassica vegetable residues, Cytotoxic effect, HPLC, Phenolics |
| title | A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues |
| title_full | A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues |
| title_fullStr | A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues |
| title_full_unstemmed | A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues |
| title_short | A Comparative Study on the Active Constituents, Antioxidant Capacity and Anti-Cancer Activity of Cruciferous Vegetable Residues |
| title_sort | comparative study on the active constituents antioxidant capacity and anti cancer activity of cruciferous vegetable residues |
| topic | Antioxidant, Brassica vegetable residues, Cytotoxic effect, HPLC, Phenolics |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3822 |
| work_keys_str_mv | AT alaaagaafar acomparativestudyontheactiveconstituentsantioxidantcapacityandanticanceractivityofcruciferousvegetableresidues AT zeinabasalama acomparativestudyontheactiveconstituentsantioxidantcapacityandanticanceractivityofcruciferousvegetableresidues AT faroukkelbaz acomparativestudyontheactiveconstituentsantioxidantcapacityandanticanceractivityofcruciferousvegetableresidues AT alaaagaafar comparativestudyontheactiveconstituentsantioxidantcapacityandanticanceractivityofcruciferousvegetableresidues AT zeinabasalama comparativestudyontheactiveconstituentsantioxidantcapacityandanticanceractivityofcruciferousvegetableresidues AT faroukkelbaz comparativestudyontheactiveconstituentsantioxidantcapacityandanticanceractivityofcruciferousvegetableresidues |