miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2
To explore the role and possible mechanism of miRNA-212 in heart failure (HF). The rat model of abdominal aortic constriction was constructed, the changes of myocardial morphology were observed by hematoxylin-eosin (HE) staining, and the hypertrophy-related marker molecules were detected by quantita...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2022/5187218 |
| _version_ | 1826833906676006912 |
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| author | Jinxia Yuan Guoliang Yuan |
| author_facet | Jinxia Yuan Guoliang Yuan |
| author_sort | Jinxia Yuan |
| collection | DOAJ |
| description | To explore the role and possible mechanism of miRNA-212 in heart failure (HF). The rat model of abdominal aortic constriction was constructed, the changes of myocardial morphology were observed by hematoxylin-eosin (HE) staining, and the hypertrophy-related marker molecules were detected by quantitative real-time polymerase chain reaction (qRT-PCR). At the cellular level, phenylephrine and angiotensin II were added to induce cardiomyocyte hypertrophy. The overexpression of miR-212 adenovirus was constructed, and the expression of miR-212 was overexpressed, and its effect on cardiac hypertrophy (CH) was detected by immunofluorescence and qRT-PCR. Then, the mechanism of miR-212 regulating CH was verified by website prediction, luciferase reporter gene assay, qRT-PCR, and western blotting assay. In the successfully constructed rat model of abdominal aortic constriction and cardiomyocyte hypertrophy, ANP and myh7 were dramatically increased, myh6 expression was decreased, and miRNA-212 expression was increased. Overexpression of miRNA-212 in cardiomyocytes can promote cardiomyocyte hypertrophy, while knocking down miR-212 in cardiomyocytes can partially reverse cell hypertrophy. In addition, miR-212 targets TCF7L2 and inhibits the expression of this gene. miRNA-212 targets TCF7L2 and inhibits the expression of this gene, possibly through this pathway to promote cardiomyocyte hypertrophy. |
| first_indexed | 2024-04-11T20:25:09Z |
| format | Article |
| id | doaj.art-c978307a6335447cbdee378d1d3c7a95 |
| institution | Directory Open Access Journal |
| issn | 1466-1861 |
| language | English |
| last_indexed | 2025-02-16T10:40:26Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj.art-c978307a6335447cbdee378d1d3c7a952025-02-03T01:24:08ZengWileyMediators of Inflammation1466-18612022-01-01202210.1155/2022/5187218miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2Jinxia Yuan0Guoliang Yuan1Department of CardiologyDepartment of CardiologyTo explore the role and possible mechanism of miRNA-212 in heart failure (HF). The rat model of abdominal aortic constriction was constructed, the changes of myocardial morphology were observed by hematoxylin-eosin (HE) staining, and the hypertrophy-related marker molecules were detected by quantitative real-time polymerase chain reaction (qRT-PCR). At the cellular level, phenylephrine and angiotensin II were added to induce cardiomyocyte hypertrophy. The overexpression of miR-212 adenovirus was constructed, and the expression of miR-212 was overexpressed, and its effect on cardiac hypertrophy (CH) was detected by immunofluorescence and qRT-PCR. Then, the mechanism of miR-212 regulating CH was verified by website prediction, luciferase reporter gene assay, qRT-PCR, and western blotting assay. In the successfully constructed rat model of abdominal aortic constriction and cardiomyocyte hypertrophy, ANP and myh7 were dramatically increased, myh6 expression was decreased, and miRNA-212 expression was increased. Overexpression of miRNA-212 in cardiomyocytes can promote cardiomyocyte hypertrophy, while knocking down miR-212 in cardiomyocytes can partially reverse cell hypertrophy. In addition, miR-212 targets TCF7L2 and inhibits the expression of this gene. miRNA-212 targets TCF7L2 and inhibits the expression of this gene, possibly through this pathway to promote cardiomyocyte hypertrophy.http://dx.doi.org/10.1155/2022/5187218 |
| spellingShingle | Jinxia Yuan Guoliang Yuan miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2 Mediators of Inflammation |
| title | miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2 |
| title_full | miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2 |
| title_fullStr | miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2 |
| title_full_unstemmed | miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2 |
| title_short | miR-212 Promotes Cardiomyocyte Hypertrophy through Regulating Transcription Factor 7 Like 2 |
| title_sort | mir 212 promotes cardiomyocyte hypertrophy through regulating transcription factor 7 like 2 |
| url | http://dx.doi.org/10.1155/2022/5187218 |
| work_keys_str_mv | AT jinxiayuan mir212promotescardiomyocytehypertrophythroughregulatingtranscriptionfactor7like2 AT guoliangyuan mir212promotescardiomyocytehypertrophythroughregulatingtranscriptionfactor7like2 |