Metabolic features of orbital adipose tissue in patients with thyroid eye disease
BackgroundThyroid eye disease (TED) is the most frequent orbital disease in adults and is characterized by the accumulation of orbital adipose tissue (OAT). It can lead to eyelid retraction or even vision loss. Orbital decompression surgery serves as the primary treatment for inactive TED by removin...
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1151757/full |
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| author | Rui Du Fenfen Wang Fenfen Wang Chun Yang Chun Yang Jing Hu Jing Hu Jiapei Liu Jiapei Liu Qizhi Jian Qizhi Jian Ruonan Wang Ruonan Wang Jian Zhang Jian Zhang Hui Chen Hui Chen Yufan Wang Fang Zhang Fang Zhang |
| author_facet | Rui Du Fenfen Wang Fenfen Wang Chun Yang Chun Yang Jing Hu Jing Hu Jiapei Liu Jiapei Liu Qizhi Jian Qizhi Jian Ruonan Wang Ruonan Wang Jian Zhang Jian Zhang Hui Chen Hui Chen Yufan Wang Fang Zhang Fang Zhang |
| author_sort | Rui Du |
| collection | DOAJ |
| description | BackgroundThyroid eye disease (TED) is the most frequent orbital disease in adults and is characterized by the accumulation of orbital adipose tissue (OAT). It can lead to eyelid retraction or even vision loss. Orbital decompression surgery serves as the primary treatment for inactive TED by removing the excess OAT. However, there is a lack of alternative treatments to surgery due to the unclear understanding of the pathogenesis, particularly the metabolic features. Accordingly, our study was implemented to explore the content and features of metabolites of OATs from TED patients.MethodThe OATs used in the current study were obtained from the orbital decompression surgery of seven patients with inactive TED. We also collected control OATs from eye surgical samples of five individuals with no history of autoimmune thyroid diseases, TED, or under non-inflammatory conditions. The liquid chromatography mass spectrometer was used for the measurements of the targeted metabolites. Afterwards, we performed differential metabolite assay analysis and related pathway enrichment analysis.ResultsIn our study, a total of 149 metabolite profiles were detected in all participants. There were significant differences in several metabolite profiles between the TED group and the control group, mainly including uric acid, oxidized glutathione, taurine, dGMP, oxidized glutathione 2, uracil, hexose-phosphate, 1-methylnicotinamide, D-sedoheptulose 1,7-bisphosphate, and uridine 5′-monophosphate (all p-value < 0.05). The TED-related pathways identified included purine metabolism, beta-alanine metabolism, glutathione metabolism (p-values < 0.05). Our study found overlaps and differences including uric acid and uracil, which are in accordance with metabolites found in blood of patients with TED from previous study and several newly discovered metabolite by our study such as hexose-phosphate, 1-methylnicotinamide, D-sedoheptulose 1,7-bisphosphate, compared to those tested from blood, OAT, or urine samples reported in previous studies.ConclusionThe findings of our study shed light on the metabolic features of OAT in individuals with TED. These results may help identify new treatment targets for TED, providing potential avenues for developing alternative treatments beyond ophthalmic surgery. |
| first_indexed | 2024-03-12T17:44:02Z |
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| language | English |
| last_indexed | 2024-03-12T17:44:02Z |
| publishDate | 2023-08-01 |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-c97a3186b4c54b4584ea59c32f95bfd32023-08-03T23:02:33ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-08-011410.3389/fendo.2023.11517571151757Metabolic features of orbital adipose tissue in patients with thyroid eye diseaseRui Du0Fenfen Wang1Fenfen Wang2Chun Yang3Chun Yang4Jing Hu5Jing Hu6Jiapei Liu7Jiapei Liu8Qizhi Jian9Qizhi Jian10Ruonan Wang11Ruonan Wang12Jian Zhang13Jian Zhang14Hui Chen15Hui Chen16Yufan Wang17Fang Zhang18Fang Zhang19Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Eye Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaBackgroundThyroid eye disease (TED) is the most frequent orbital disease in adults and is characterized by the accumulation of orbital adipose tissue (OAT). It can lead to eyelid retraction or even vision loss. Orbital decompression surgery serves as the primary treatment for inactive TED by removing the excess OAT. However, there is a lack of alternative treatments to surgery due to the unclear understanding of the pathogenesis, particularly the metabolic features. Accordingly, our study was implemented to explore the content and features of metabolites of OATs from TED patients.MethodThe OATs used in the current study were obtained from the orbital decompression surgery of seven patients with inactive TED. We also collected control OATs from eye surgical samples of five individuals with no history of autoimmune thyroid diseases, TED, or under non-inflammatory conditions. The liquid chromatography mass spectrometer was used for the measurements of the targeted metabolites. Afterwards, we performed differential metabolite assay analysis and related pathway enrichment analysis.ResultsIn our study, a total of 149 metabolite profiles were detected in all participants. There were significant differences in several metabolite profiles between the TED group and the control group, mainly including uric acid, oxidized glutathione, taurine, dGMP, oxidized glutathione 2, uracil, hexose-phosphate, 1-methylnicotinamide, D-sedoheptulose 1,7-bisphosphate, and uridine 5′-monophosphate (all p-value < 0.05). The TED-related pathways identified included purine metabolism, beta-alanine metabolism, glutathione metabolism (p-values < 0.05). Our study found overlaps and differences including uric acid and uracil, which are in accordance with metabolites found in blood of patients with TED from previous study and several newly discovered metabolite by our study such as hexose-phosphate, 1-methylnicotinamide, D-sedoheptulose 1,7-bisphosphate, compared to those tested from blood, OAT, or urine samples reported in previous studies.ConclusionThe findings of our study shed light on the metabolic features of OAT in individuals with TED. These results may help identify new treatment targets for TED, providing potential avenues for developing alternative treatments beyond ophthalmic surgery.https://www.frontiersin.org/articles/10.3389/fendo.2023.1151757/fullthyroid eye diseasemetabolic featuresorbital adipose tissuetreatment targetspathogenesis |
| spellingShingle | Rui Du Fenfen Wang Fenfen Wang Chun Yang Chun Yang Jing Hu Jing Hu Jiapei Liu Jiapei Liu Qizhi Jian Qizhi Jian Ruonan Wang Ruonan Wang Jian Zhang Jian Zhang Hui Chen Hui Chen Yufan Wang Fang Zhang Fang Zhang Metabolic features of orbital adipose tissue in patients with thyroid eye disease Frontiers in Endocrinology thyroid eye disease metabolic features orbital adipose tissue treatment targets pathogenesis |
| title | Metabolic features of orbital adipose tissue in patients with thyroid eye disease |
| title_full | Metabolic features of orbital adipose tissue in patients with thyroid eye disease |
| title_fullStr | Metabolic features of orbital adipose tissue in patients with thyroid eye disease |
| title_full_unstemmed | Metabolic features of orbital adipose tissue in patients with thyroid eye disease |
| title_short | Metabolic features of orbital adipose tissue in patients with thyroid eye disease |
| title_sort | metabolic features of orbital adipose tissue in patients with thyroid eye disease |
| topic | thyroid eye disease metabolic features orbital adipose tissue treatment targets pathogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1151757/full |
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