Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro
Abstract Background Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse’s space, being the major site of vitamin A storage. When activated, they convert t...
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| Format: | Article |
| Language: | English |
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BMC
2015-11-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-015-1197-3 |
| _version_ | 1797811780901142528 |
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| author | Ligia Almeida Paiva Camila Brand Christianne Bandeira-Melo Patricia Torres Bozza Marcia Cury El-Cheikh Patricia Martins Silva Radovan Borojevic Sandra Aurora Chavez Perez |
| author_facet | Ligia Almeida Paiva Camila Brand Christianne Bandeira-Melo Patricia Torres Bozza Marcia Cury El-Cheikh Patricia Martins Silva Radovan Borojevic Sandra Aurora Chavez Perez |
| author_sort | Ligia Almeida Paiva |
| collection | DOAJ |
| description | Abstract Background Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse’s space, being the major site of vitamin A storage. When activated, they convert to myofibroblasts and contribute to granulomatous and diffuse liver fibrosis. In the present work, we observed that myofibroblasts obtained from granulomatous periovular inflammatory reactions in schistosome-infected mice (GR-MF) produce in vitro immunomodulatory cytokines for eosinophil activation: IL-5 and eotaxin. Methods and results The secretory activity of GR-MF was detected after TGF-β and IL-13 stimulation using 2D and 3D cell culture systems. In a mixed co-culture system using GR-MF with hematopoietic bone marrow cells from infected mice, we observed eosinophil survival that was dependent upon IL-5 and eotaxin, since antibodies against this cytokines decreased eosinophil population, as measured by eosinophil peroxidase activity. Conclusion These results indicate that GR-MF may contribute to maintenance of local eosinophilia in schistosomal hepatic granulomas, and can function as immunoregulatory cells, besides their role in production of fibrosis. |
| first_indexed | 2024-03-13T07:28:48Z |
| format | Article |
| id | doaj.art-c97dbe118eec47b7bd67e10e579468e2 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:28:48Z |
| publishDate | 2015-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c97dbe118eec47b7bd67e10e579468e22023-06-04T11:14:22ZengBMCParasites & Vectors1756-33052015-11-018111010.1186/s13071-015-1197-3Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitroLigia Almeida Paiva0Camila Brand1Christianne Bandeira-Melo2Patricia Torres Bozza3Marcia Cury El-Cheikh4Patricia Martins Silva5Radovan Borojevic6Sandra Aurora Chavez Perez7Laboratory of Immunopharmacology, Institute Oswaldo Cruz, FIOCRUZInstitute of Biomedical Sciences, Federal University of Rio de JaneiroInstitute of Biophysics Carlos Chagas Filho, Federal University of Rio de JaneiroLaboratory of Immunopharmacology, Institute Oswaldo Cruz, FIOCRUZInstitute of Biomedical Sciences, Federal University of Rio de JaneiroLaboratory of Inflammation, Institute Oswaldo Cruz, FIOCRUZFaculty of Medicine / FASEFarmanguinhos, FIOCRUZAbstract Background Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse’s space, being the major site of vitamin A storage. When activated, they convert to myofibroblasts and contribute to granulomatous and diffuse liver fibrosis. In the present work, we observed that myofibroblasts obtained from granulomatous periovular inflammatory reactions in schistosome-infected mice (GR-MF) produce in vitro immunomodulatory cytokines for eosinophil activation: IL-5 and eotaxin. Methods and results The secretory activity of GR-MF was detected after TGF-β and IL-13 stimulation using 2D and 3D cell culture systems. In a mixed co-culture system using GR-MF with hematopoietic bone marrow cells from infected mice, we observed eosinophil survival that was dependent upon IL-5 and eotaxin, since antibodies against this cytokines decreased eosinophil population, as measured by eosinophil peroxidase activity. Conclusion These results indicate that GR-MF may contribute to maintenance of local eosinophilia in schistosomal hepatic granulomas, and can function as immunoregulatory cells, besides their role in production of fibrosis.https://doi.org/10.1186/s13071-015-1197-3Liver myofibroblastsIL-5EotaxinSchistosoma mansoniEosinophil granulocyte |
| spellingShingle | Ligia Almeida Paiva Camila Brand Christianne Bandeira-Melo Patricia Torres Bozza Marcia Cury El-Cheikh Patricia Martins Silva Radovan Borojevic Sandra Aurora Chavez Perez Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro Parasites & Vectors Liver myofibroblasts IL-5 Eotaxin Schistosoma mansoni Eosinophil granulocyte |
| title | Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro |
| title_full | Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro |
| title_fullStr | Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro |
| title_full_unstemmed | Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro |
| title_short | Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro |
| title_sort | hepatic myofibroblasts derived from schistosoma mansoni infected mice are a source of il 5 and eotaxin controls of eosinophil populations in vitro |
| topic | Liver myofibroblasts IL-5 Eotaxin Schistosoma mansoni Eosinophil granulocyte |
| url | https://doi.org/10.1186/s13071-015-1197-3 |
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