Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities
In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1345423/full |
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| author | Ebrahim Saied Mostafa A. Abdel-Maksoud Akram A. Alfuraydi Bushra Hafeez Kiani Mohamed Bassyouni Mohamed Bassyouni Osama A. Al-Qabandi Fathia H. E. Bougafa Mona Shaban E. M. Badawy Amr H. Hashem |
| author_facet | Ebrahim Saied Mostafa A. Abdel-Maksoud Akram A. Alfuraydi Bushra Hafeez Kiani Mohamed Bassyouni Mohamed Bassyouni Osama A. Al-Qabandi Fathia H. E. Bougafa Mona Shaban E. M. Badawy Amr H. Hashem |
| author_sort | Ebrahim Saied |
| collection | DOAJ |
| description | In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and UV–Vis spectroscopy. The obtained results demonstrated the successful formation of crystalline, spherical Ag-NPs with particle diameters ranging from 16 to 31 nm. The FT-IR studied and displayed the various functional groups involved, which played a role in capping and reducing agents for Ag-NPs production. The SEM–EDX revealed that the main constituent of the AS-formed sample was primarily Ag, with a weight percentage of 64.2%. The mycosynthesized Ag-NPs were assessed for antimicrobial as well as photocatalytic activities. The antimicrobial results indicated that the synthesized Ag-NPs possess notable antibacterial efficacy against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, with minimum inhibitory concentrations (MICs) of Ag-NPs ranging from 62.5 to 250 μg/mL. Moreover, the biosynthesized Ag-NPs demonstrated weak antifungal activity against Aspergillus brasiliensis and Candida albicans, with MICs of 500 and 1,000 μg/mL, respectively. In addition, the mycosynthesized Ag-NPs exhibited photocatalytic activity toward acid black 2 (nigrosine) dye under both light and dark stimulation. Notably, After 300 min exposure to light, the nigrosine dye was degraded by 93%. In contrast, 51% degradation was observed after 300 min in darkness. In conclusion, Ag-NPs were successfully biosynthesized using endophytic A. hiratsukae and also exhibited antimicrobial and photocatalytic activities that can be used in environmental applications. |
| first_indexed | 2024-04-25T00:37:59Z |
| format | Article |
| id | doaj.art-c97ffb1cd39e4399a28ed7b493888c17 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-25T00:37:59Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c97ffb1cd39e4399a28ed7b493888c172024-03-12T13:23:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13454231345423Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activitiesEbrahim Saied0Mostafa A. Abdel-Maksoud1Akram A. Alfuraydi2Bushra Hafeez Kiani3Mohamed Bassyouni4Mohamed Bassyouni5Osama A. Al-Qabandi6Fathia H. E. Bougafa7Mona Shaban E. M. Badawy8Amr H. Hashem9Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, United StatesDepartment of Chemical Engineering, Faculty of Engineering, Port Said University, Port Said, EgyptCenter of Excellence in Membrane-Based Water Desalination Technology for Testing and Characterization (CEMTC), Port Said University, Port Said, EgyptCollege of Engineering and Technology, American University of the Middle East, Egaila, KuwaitDepartment of Microbiology, Faculty of Science, Tobruk University, Tobruk, LibyaDepartment of Microbiology and Immunology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, EgyptIn the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and UV–Vis spectroscopy. The obtained results demonstrated the successful formation of crystalline, spherical Ag-NPs with particle diameters ranging from 16 to 31 nm. The FT-IR studied and displayed the various functional groups involved, which played a role in capping and reducing agents for Ag-NPs production. The SEM–EDX revealed that the main constituent of the AS-formed sample was primarily Ag, with a weight percentage of 64.2%. The mycosynthesized Ag-NPs were assessed for antimicrobial as well as photocatalytic activities. The antimicrobial results indicated that the synthesized Ag-NPs possess notable antibacterial efficacy against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, with minimum inhibitory concentrations (MICs) of Ag-NPs ranging from 62.5 to 250 μg/mL. Moreover, the biosynthesized Ag-NPs demonstrated weak antifungal activity against Aspergillus brasiliensis and Candida albicans, with MICs of 500 and 1,000 μg/mL, respectively. In addition, the mycosynthesized Ag-NPs exhibited photocatalytic activity toward acid black 2 (nigrosine) dye under both light and dark stimulation. Notably, After 300 min exposure to light, the nigrosine dye was degraded by 93%. In contrast, 51% degradation was observed after 300 min in darkness. In conclusion, Ag-NPs were successfully biosynthesized using endophytic A. hiratsukae and also exhibited antimicrobial and photocatalytic activities that can be used in environmental applications.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1345423/fullantimicrobial activitybiosynthesisfungal endophytesphotocatalytic activitysilver nanoparticles |
| spellingShingle | Ebrahim Saied Mostafa A. Abdel-Maksoud Akram A. Alfuraydi Bushra Hafeez Kiani Mohamed Bassyouni Mohamed Bassyouni Osama A. Al-Qabandi Fathia H. E. Bougafa Mona Shaban E. M. Badawy Amr H. Hashem Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities Frontiers in Microbiology antimicrobial activity biosynthesis fungal endophytes photocatalytic activity silver nanoparticles |
| title | Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities |
| title_full | Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities |
| title_fullStr | Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities |
| title_full_unstemmed | Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities |
| title_short | Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities |
| title_sort | endophytic aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities |
| topic | antimicrobial activity biosynthesis fungal endophytes photocatalytic activity silver nanoparticles |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1345423/full |
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