Engineering Escherichia coli for renewable benzyl alcohol production
Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escheri...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2015-12-01
|
Series: | Metabolic Engineering Communications |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030115300018 |
_version_ | 1811209987164209152 |
---|---|
author | Shawn Pugh Rebekah McKenna Ibrahim Halloum David R. Nielsen |
author_facet | Shawn Pugh Rebekah McKenna Ibrahim Halloum David R. Nielsen |
author_sort | Shawn Pugh |
collection | DOAJ |
description | Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1 mg/L were realized, representing a yield of 7.6±0.1 mg/g on glucose and a ~5-fold improvement over initial strains. Keywords: Benzyl alcohol, Benzaldehyde, Aromatic chemicals |
first_indexed | 2024-04-12T04:49:15Z |
format | Article |
id | doaj.art-c9810ac63b0c479ba2a5adb0af8389ff |
institution | Directory Open Access Journal |
issn | 2214-0301 |
language | English |
last_indexed | 2024-04-12T04:49:15Z |
publishDate | 2015-12-01 |
publisher | Elsevier |
record_format | Article |
series | Metabolic Engineering Communications |
spelling | doaj.art-c9810ac63b0c479ba2a5adb0af8389ff2022-12-22T03:47:23ZengElsevierMetabolic Engineering Communications2214-03012015-12-0123945Engineering Escherichia coli for renewable benzyl alcohol productionShawn Pugh0Rebekah McKenna1Ibrahim Halloum2David R. Nielsen3Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, PO Box 876106, Tempe, AZ, 85287-6106, United StatesChemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, PO Box 876106, Tempe, AZ, 85287-6106, United StatesChemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, PO Box 876106, Tempe, AZ, 85287-6106, United StatesCorresponding author. Fax: +1 4807279321.; Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, PO Box 876106, Tempe, AZ, 85287-6106, United StatesBenzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1 mg/L were realized, representing a yield of 7.6±0.1 mg/g on glucose and a ~5-fold improvement over initial strains. Keywords: Benzyl alcohol, Benzaldehyde, Aromatic chemicalshttp://www.sciencedirect.com/science/article/pii/S2214030115300018 |
spellingShingle | Shawn Pugh Rebekah McKenna Ibrahim Halloum David R. Nielsen Engineering Escherichia coli for renewable benzyl alcohol production Metabolic Engineering Communications |
title | Engineering Escherichia coli for renewable benzyl alcohol production |
title_full | Engineering Escherichia coli for renewable benzyl alcohol production |
title_fullStr | Engineering Escherichia coli for renewable benzyl alcohol production |
title_full_unstemmed | Engineering Escherichia coli for renewable benzyl alcohol production |
title_short | Engineering Escherichia coli for renewable benzyl alcohol production |
title_sort | engineering escherichia coli for renewable benzyl alcohol production |
url | http://www.sciencedirect.com/science/article/pii/S2214030115300018 |
work_keys_str_mv | AT shawnpugh engineeringescherichiacoliforrenewablebenzylalcoholproduction AT rebekahmckenna engineeringescherichiacoliforrenewablebenzylalcoholproduction AT ibrahimhalloum engineeringescherichiacoliforrenewablebenzylalcoholproduction AT davidrnielsen engineeringescherichiacoliforrenewablebenzylalcoholproduction |