Anticancer activity of ethanolic extract of propolis on AGS cell line
Introduction: Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries. Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidan...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2015-01-01
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| Series: | Journal of HerbMed Pharmacology |
| Subjects: | |
| Online Access: | http://www.herbmedpharmacol.com/PDF/JHP-4-29.pdf |
| _version_ | 1828424661987229696 |
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| author | Amini-Sarteshnizi Nematallah Mobini-Dehkordi Mohsen Khosravi-Farsani Somayeh Teimori Hossein |
| author_facet | Amini-Sarteshnizi Nematallah Mobini-Dehkordi Mohsen Khosravi-Farsani Somayeh Teimori Hossein |
| author_sort | Amini-Sarteshnizi Nematallah |
| collection | DOAJ |
| description | Introduction: Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries. Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties. The aim of this study was to investigate the effect of ethanolic extract of propolis (EEP) obtained from Dinaran area (Iran) on AGS human gastric cancer cell line. Methods: The ethanolic extract of samples was obtained by ethanol 96% and pure extract was dissolved in dimethyl sulfoxide (DMSO) and used for experiments. The cytotoxic effects of various concentrations of EEP on AGS cells were investigated by MTT assay test after 24, 48, and 72 hours and compared with control cells. Results: The EEP inhibited the growth and proliferation of AGS human gastric cancer cell line. The antiproliferative effects were revealed in a dose and time-dependent manner. The IC50 values were recorded as 60, 30, and 15 (μg/ml) in treatment times of 24, 48 and 72 hours, respectively. Conclusion: These findings indicated that the native EEP has strong antiproliferative effects against cancerous AGS cells. Thus, propolis and related products may provide a novel approach to the chemoprevention and treatment of human gastric cancer. |
| first_indexed | 2024-12-10T16:21:15Z |
| format | Article |
| id | doaj.art-c98123d3d74844a1978fb134034c3878 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-12-10T16:21:15Z |
| publishDate | 2015-01-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-c98123d3d74844a1978fb134034c38782022-12-22T01:41:49ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042015-01-01412934JHP_20150527174303Anticancer activity of ethanolic extract of propolis on AGS cell lineAmini-Sarteshnizi Nematallah0Mobini-Dehkordi Mohsen1Khosravi-Farsani Somayeh2Teimori Hossein3MSc Student of Cellular and Molecular Biology, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.Assistant Professor of Molecular Microbiology, Genetics Department, Faculty of Science, University of Shahrekord, Shahrekord, Iran.Assistant Professor of Anatomical Sciences, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranAssistant Professor of Medical Genetics, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranIntroduction: Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries. Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties. The aim of this study was to investigate the effect of ethanolic extract of propolis (EEP) obtained from Dinaran area (Iran) on AGS human gastric cancer cell line. Methods: The ethanolic extract of samples was obtained by ethanol 96% and pure extract was dissolved in dimethyl sulfoxide (DMSO) and used for experiments. The cytotoxic effects of various concentrations of EEP on AGS cells were investigated by MTT assay test after 24, 48, and 72 hours and compared with control cells. Results: The EEP inhibited the growth and proliferation of AGS human gastric cancer cell line. The antiproliferative effects were revealed in a dose and time-dependent manner. The IC50 values were recorded as 60, 30, and 15 (μg/ml) in treatment times of 24, 48 and 72 hours, respectively. Conclusion: These findings indicated that the native EEP has strong antiproliferative effects against cancerous AGS cells. Thus, propolis and related products may provide a novel approach to the chemoprevention and treatment of human gastric cancer.http://www.herbmedpharmacol.com/PDF/JHP-4-29.pdfPropolisAGSMTTViability |
| spellingShingle | Amini-Sarteshnizi Nematallah Mobini-Dehkordi Mohsen Khosravi-Farsani Somayeh Teimori Hossein Anticancer activity of ethanolic extract of propolis on AGS cell line Journal of HerbMed Pharmacology Propolis AGSMTT Viability |
| title | Anticancer activity of ethanolic extract of propolis on AGS cell line |
| title_full | Anticancer activity of ethanolic extract of propolis on AGS cell line |
| title_fullStr | Anticancer activity of ethanolic extract of propolis on AGS cell line |
| title_full_unstemmed | Anticancer activity of ethanolic extract of propolis on AGS cell line |
| title_short | Anticancer activity of ethanolic extract of propolis on AGS cell line |
| title_sort | anticancer activity of ethanolic extract of propolis on ags cell line |
| topic | Propolis AGSMTT Viability |
| url | http://www.herbmedpharmacol.com/PDF/JHP-4-29.pdf |
| work_keys_str_mv | AT aminisarteshnizinematallah anticanceractivityofethanolicextractofpropolisonagscellline AT mobinidehkordimohsen anticanceractivityofethanolicextractofpropolisonagscellline AT khosravifarsanisomayeh anticanceractivityofethanolicextractofpropolisonagscellline AT teimorihossein anticanceractivityofethanolicextractofpropolisonagscellline |