A novel view of the problem of Osteoarthritis in experimental rat model
Introduction: The article presents the results of the functional tests to improve the assessment of MIA-induced osteoarthritis development and the effectiveness of NSAID therapy. Materials and methods: In the study, 26 male SD rats were used. MIA-induced osteoarthritis was simulated...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Belgorod National Research University
2020-06-01
|
| Series: | Research Results in Pharmacology |
| Online Access: | https://rrpharmacology.pensoft.net/article/51772/download/pdf/ |
| _version_ | 1797429445654478848 |
|---|---|
| author | Viktor A. Palikov Yuliya A. Palikova Natalya A. Borozdina Elena N. Nesmeyanova Pavel A. Rudenko Vitalij A. Kazakov Elena A. Kalabina Mikhail V. Bukatin Tatyana Yu. Zharmukhamedova Oksana N. Khokhlova Igor A. Dyachenko |
| author_facet | Viktor A. Palikov Yuliya A. Palikova Natalya A. Borozdina Elena N. Nesmeyanova Pavel A. Rudenko Vitalij A. Kazakov Elena A. Kalabina Mikhail V. Bukatin Tatyana Yu. Zharmukhamedova Oksana N. Khokhlova Igor A. Dyachenko |
| author_sort | Viktor A. Palikov |
| collection | DOAJ |
| description | Introduction: The article presents the results of the functional tests to improve the assessment of MIA-induced osteoarthritis development and the effectiveness of NSAID therapy. Materials and methods: In the study, 26 male SD rats were used. MIA-induced osteoarthritis was simulated in the right knee joint. After an intra-articular injection of MIA, the animals were treated with ibuprofen and meloxicam. Pain assessment was studied in the following functional tests: incapacitance (hind limb weight bearing) test, von Frey test (mechanical allodynia), grip strength test, and knee diameter measurement. At the end of the study, a histological analysis of the knee joint was performed. Results and discussion: An intra-articular MIA injection reduced 1.5 times the paw withdrawal threshold. In the rats that suffered MIA-induced osteoarthritis, the difference between the diameters of the intact and injected joints was 1.05 mm, compared to 0.03 mm difference in the control group. Hind limb weight bearing asymmetry was 89.5% when simulating MIA-induced osteoarthritis. The muscular hind limb grip strength in rats with MIA-induced osteoarthritis was significantly reduced on 3rd and 7th days after simulating osteoarthritis. Ibuprofen and meloxicam showed significant efficacy in all the above tests, although ibuprofen effectiveness was more pronounced than that of meloxicam. Conclusion: The following functional tests were identified as the most significant and sufficient to assess the development of MIA-induced osteoarthritis and analgesic efficacy of NSAIDs: incapacitance test, allodynia test (von Frey filaments), measurement of hind limb grip strength and measurement of the diameter of the inflamed knee joint. The histological analysis made it possible to confirm the correspondence of the physiological response and pathological changes in the knee joint. |
| first_indexed | 2024-03-09T09:13:16Z |
| format | Article |
| id | doaj.art-c98127e98cbe4cf3a35a42b01353155a |
| institution | Directory Open Access Journal |
| issn | 2658-381X |
| language | English |
| last_indexed | 2024-03-09T09:13:16Z |
| publishDate | 2020-06-01 |
| publisher | Belgorod National Research University |
| record_format | Article |
| series | Research Results in Pharmacology |
| spelling | doaj.art-c98127e98cbe4cf3a35a42b01353155a2023-12-02T07:55:34ZengBelgorod National Research UniversityResearch Results in Pharmacology2658-381X2020-06-0162192510.3897/rrpharmacology.6.5177251772A novel view of the problem of Osteoarthritis in experimental rat modelViktor A. Palikov0Yuliya A. Palikova1Natalya A. Borozdina2Elena N. Nesmeyanova3Pavel A. Rudenko4Vitalij A. Kazakov5Elena A. Kalabina6Mikhail V. Bukatin7Tatyana Yu. Zharmukhamedova8Oksana N. Khokhlova9Igor A. Dyachenko10Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesVolgograd State UniversityPushchino State Institute of Natural SciencesBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesVolgograd State Medical UniversityBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesIntroduction: The article presents the results of the functional tests to improve the assessment of MIA-induced osteoarthritis development and the effectiveness of NSAID therapy. Materials and methods: In the study, 26 male SD rats were used. MIA-induced osteoarthritis was simulated in the right knee joint. After an intra-articular injection of MIA, the animals were treated with ibuprofen and meloxicam. Pain assessment was studied in the following functional tests: incapacitance (hind limb weight bearing) test, von Frey test (mechanical allodynia), grip strength test, and knee diameter measurement. At the end of the study, a histological analysis of the knee joint was performed. Results and discussion: An intra-articular MIA injection reduced 1.5 times the paw withdrawal threshold. In the rats that suffered MIA-induced osteoarthritis, the difference between the diameters of the intact and injected joints was 1.05 mm, compared to 0.03 mm difference in the control group. Hind limb weight bearing asymmetry was 89.5% when simulating MIA-induced osteoarthritis. The muscular hind limb grip strength in rats with MIA-induced osteoarthritis was significantly reduced on 3rd and 7th days after simulating osteoarthritis. Ibuprofen and meloxicam showed significant efficacy in all the above tests, although ibuprofen effectiveness was more pronounced than that of meloxicam. Conclusion: The following functional tests were identified as the most significant and sufficient to assess the development of MIA-induced osteoarthritis and analgesic efficacy of NSAIDs: incapacitance test, allodynia test (von Frey filaments), measurement of hind limb grip strength and measurement of the diameter of the inflamed knee joint. The histological analysis made it possible to confirm the correspondence of the physiological response and pathological changes in the knee joint.https://rrpharmacology.pensoft.net/article/51772/download/pdf/ |
| spellingShingle | Viktor A. Palikov Yuliya A. Palikova Natalya A. Borozdina Elena N. Nesmeyanova Pavel A. Rudenko Vitalij A. Kazakov Elena A. Kalabina Mikhail V. Bukatin Tatyana Yu. Zharmukhamedova Oksana N. Khokhlova Igor A. Dyachenko A novel view of the problem of Osteoarthritis in experimental rat model Research Results in Pharmacology |
| title | A novel view of the problem of Osteoarthritis in experimental rat model |
| title_full | A novel view of the problem of Osteoarthritis in experimental rat model |
| title_fullStr | A novel view of the problem of Osteoarthritis in experimental rat model |
| title_full_unstemmed | A novel view of the problem of Osteoarthritis in experimental rat model |
| title_short | A novel view of the problem of Osteoarthritis in experimental rat model |
| title_sort | novel view of the problem of osteoarthritis in experimental rat model |
| url | https://rrpharmacology.pensoft.net/article/51772/download/pdf/ |
| work_keys_str_mv | AT viktorapalikov anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT yuliyaapalikova anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT natalyaaborozdina anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT elenannesmeyanova anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT pavelarudenko anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT vitalijakazakov anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT elenaakalabina anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT mikhailvbukatin anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT tatyanayuzharmukhamedova anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT oksanankhokhlova anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT igoradyachenko anovelviewoftheproblemofosteoarthritisinexperimentalratmodel AT viktorapalikov novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT yuliyaapalikova novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT natalyaaborozdina novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT elenannesmeyanova novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT pavelarudenko novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT vitalijakazakov novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT elenaakalabina novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT mikhailvbukatin novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT tatyanayuzharmukhamedova novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT oksanankhokhlova novelviewoftheproblemofosteoarthritisinexperimentalratmodel AT igoradyachenko novelviewoftheproblemofosteoarthritisinexperimentalratmodel |