Cyp19 expression and sex change timing in captive-bred false clownfish
Abstract The false clown anemonefish (Amphiprion ocellaris) is a protandrous hermaphrodite with a distinctive reproductive behavior. This study elucidates the genetic mechanisms and timing of sex changes in captive-bred A. ocellaris by examining the expression of key genes involved in this process,...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2023-08-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100823&tlng=en |
| _version_ | 1797743337104474112 |
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| author | J. Thamnawasolos C. Boonphakdee |
| author_facet | J. Thamnawasolos C. Boonphakdee |
| author_sort | J. Thamnawasolos |
| collection | DOAJ |
| description | Abstract The false clown anemonefish (Amphiprion ocellaris) is a protandrous hermaphrodite with a distinctive reproductive behavior. This study elucidates the genetic mechanisms and timing of sex changes in captive-bred A. ocellaris by examining the expression of key genes involved in this process, specifically cyp19a1a and cyp19a1b. Gonadal histological analyses and gene expression studies were conducted on subadult fish paired for 0, 1, 2, 3, 4, 5, and 16 months. Our findings reveal that alterations in cyp19 gene expression coincide with a pairing period starting after 3 months. Both cyp19a1a and cyp19a1b expression levels were significantly elevated in paired females compared with their male counterparts and unpaired controls. Histological investigations demonstrated that sex conversion to females occurred during the 3-month pairing period. This study highlights the crucial role of cyp19a1a and cyp19a1b in the sex change process of A. ocellaris and indicates that a minimum of 5 months of pairing is necessary for completing the sex change. |
| first_indexed | 2024-03-12T14:54:01Z |
| format | Article |
| id | doaj.art-c98257d3f6b84ce79cace075726f8e9c |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-03-12T14:54:01Z |
| publishDate | 2023-08-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-c98257d3f6b84ce79cace075726f8e9c2023-08-15T07:44:47ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752023-08-018310.1590/1519-6984.273824Cyp19 expression and sex change timing in captive-bred false clownfishJ. Thamnawasoloshttps://orcid.org/0009-0003-5281-9464C. Boonphakdeehttps://orcid.org/0000-0002-8706-225XAbstract The false clown anemonefish (Amphiprion ocellaris) is a protandrous hermaphrodite with a distinctive reproductive behavior. This study elucidates the genetic mechanisms and timing of sex changes in captive-bred A. ocellaris by examining the expression of key genes involved in this process, specifically cyp19a1a and cyp19a1b. Gonadal histological analyses and gene expression studies were conducted on subadult fish paired for 0, 1, 2, 3, 4, 5, and 16 months. Our findings reveal that alterations in cyp19 gene expression coincide with a pairing period starting after 3 months. Both cyp19a1a and cyp19a1b expression levels were significantly elevated in paired females compared with their male counterparts and unpaired controls. Histological investigations demonstrated that sex conversion to females occurred during the 3-month pairing period. This study highlights the crucial role of cyp19a1a and cyp19a1b in the sex change process of A. ocellaris and indicates that a minimum of 5 months of pairing is necessary for completing the sex change.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100823&tlng=enanemonefishclownfishcyp19a1acyp19a1bsex change |
| spellingShingle | J. Thamnawasolos C. Boonphakdee Cyp19 expression and sex change timing in captive-bred false clownfish Brazilian Journal of Biology anemonefish clownfish cyp19a1a cyp19a1b sex change |
| title | Cyp19 expression and sex change timing in captive-bred false clownfish |
| title_full | Cyp19 expression and sex change timing in captive-bred false clownfish |
| title_fullStr | Cyp19 expression and sex change timing in captive-bred false clownfish |
| title_full_unstemmed | Cyp19 expression and sex change timing in captive-bred false clownfish |
| title_short | Cyp19 expression and sex change timing in captive-bred false clownfish |
| title_sort | cyp19 expression and sex change timing in captive bred false clownfish |
| topic | anemonefish clownfish cyp19a1a cyp19a1b sex change |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100823&tlng=en |
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