Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding
Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic aci...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2005-09-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200503-0004_development-of-self-incompatible-lines-with-improved-seed-quality-in-winter-oilseed-rape-brassica-napus-l-fo.php |
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| author | R Koprna V Kučera O Kolovrat M Vyvadilová M Klíma |
| author_facet | R Koprna V Kučera O Kolovrat M Vyvadilová M Klíma |
| author_sort | R Koprna |
| collection | DOAJ |
| description | Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified. |
| first_indexed | 2024-04-10T08:30:56Z |
| format | Article |
| id | doaj.art-c982feeacb4a4117b697164f4315325d |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:30:56Z |
| publishDate | 2005-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-c982feeacb4a4117b697164f4315325d2023-02-23T03:29:09ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252005-09-0141310511110.17221/3668-CJGPBcjg-200503-0004Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid BreedingR Koprna0V Kučera1O Kolovrat2M Vyvadilová3M Klíma4OSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech RepublicDepartment of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicOSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech RepublicDepartment of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicDepartment of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicDoubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified.https://cjgpb.agriculturejournals.cz/artkey/cjg-200503-0004_development-of-self-incompatible-lines-with-improved-seed-quality-in-winter-oilseed-rape-brassica-napus-l-fo.phpoilseed rape (brassica napus l. var. napus)hybrid breedingself-incompatibilityseed qualityglucosinolateserucic acid |
| spellingShingle | R Koprna V Kučera O Kolovrat M Vyvadilová M Klíma Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding Czech Journal of Genetics and Plant Breeding oilseed rape (brassica napus l. var. napus) hybrid breeding self-incompatibility seed quality glucosinolates erucic acid |
| title | Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding |
| title_full | Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding |
| title_fullStr | Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding |
| title_full_unstemmed | Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding |
| title_short | Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding |
| title_sort | development of self incompatible lines with improved seed quality in winter oilseed rape brassica napus l for hybrid breeding |
| topic | oilseed rape (brassica napus l. var. napus) hybrid breeding self-incompatibility seed quality glucosinolates erucic acid |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200503-0004_development-of-self-incompatible-lines-with-improved-seed-quality-in-winter-oilseed-rape-brassica-napus-l-fo.php |
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