p63 as an Ideal Diagnostic Marker for Pleomorphic Adenoma: An Immunohistochemical Study
ntroduction: Pleomorphic Adenoma (PA) histopathologically represents a heterogeneous lesion with a varying proportion of epithelial and mesenchymal components. Due to its morphological diversity, a diagnostic dilemma often arises when identifying its various patterns. This diverse morphology is...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2023-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://www.jcdr.net/articles/PDF/18554/61190_CE[Ra1]_F[SK]_QC(AN_OM)_PF1(HB_OM)_PFA(KM)_PB(HB_SL_OM)_PN(KM).pdf |
| Summary: | ntroduction: Pleomorphic Adenoma (PA) histopathologically
represents a heterogeneous lesion with a varying proportion
of epithelial and mesenchymal components. Due to its
morphological diversity, a diagnostic dilemma often arises
when identifying its various patterns. This diverse morphology
is considered to be a function of the neoplastic myoepithelium.
Tumor protein 63 (p63) is expressed in stratified epithelia and in
the basal cells of salivary glands.
Aim: To investigate the immunoreactivity of p63 as a reliable
myoepithelial marker in PA.
Materials and Methods: A cross-sectional study was performed
in the Department of Oral and Maxillofacial Pathology at PGIDS,
Rohtak, Haryana, India. The duration of the study was 14 months,
from March 2015 to April 2016. A total of 15 tissue blocks of
histopathologically diagnosed cases of PA were included from
departmental archives and subjected to Immunohistochemistry
(IHC) using a monoclonal p63 antibody. The myoepithelium was
classified as myoepithelial-like (abluminal and spindled), modified
myoepithelium (myxoid and chondroid), and transformed
myoepithelium (solid epithelioid, squamous, and basaloid
cribriform). IHC for p63 was assessed in each myoepithelial
component, as well as in non neoplastic Myoepithelial Cells
(MEC) and inner tubular epithelial cells. Only nuclear staining for
p63 was considered positive. The obtained data were subjected
to statistical analysis, and the Chi-square test was applied.
Results: The PA samples subjected to IHC showed 100% p63
reactivity. Transformed MEC, abluminal, and modified MEC
revealed variable immunostaining with a significant difference
(p=0.049). There was no immunostaining in luminal/inner layer
cells in all cases of PA. p63 was also expressed in the nuclei
of MEC of acini and intercalated ducts of normal salivary
gland tissue.
Conclusion: p63 is a sensitive and specific myoepithelial marker
to identify MEC in PA. Additionally, the expression of MECassociated markers in acini and intercalated ducts of normal
salivary glands has confirmed their role in the histogenesis of
this tumour. |
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| ISSN: | 2249-782X 0973-709X |