Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon.
Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ pr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5132261?pdf=render |
| _version_ | 1811208828280111104 |
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| author | Ryan T Terry-Lorenzo Viviana I Torres Dhananjay Wagh Jose Galaz Selene K Swanson Laurence Florens Michael P Washburn Clarissa L Waites Eckart D Gundelfinger Richard J Reimer Craig C Garner |
| author_facet | Ryan T Terry-Lorenzo Viviana I Torres Dhananjay Wagh Jose Galaz Selene K Swanson Laurence Florens Michael P Washburn Clarissa L Waites Eckart D Gundelfinger Richard J Reimer Craig C Garner |
| author_sort | Ryan T Terry-Lorenzo |
| collection | DOAJ |
| description | Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate the dynamic assembly of actin and growth factor dependent axon guidance and synaptic growth. Trio was found to interact with the C-terminal PBH 9/10 domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a domain that is also critical for its localization to the CAZ. Moreover, our data suggest that regions within the C-terminus of Trio negatively regulate its interactions with Piccolo/Bassoon. These findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis. |
| first_indexed | 2024-04-12T04:28:17Z |
| format | Article |
| id | doaj.art-c98af2f3e56245ee872e4c61b972e3f2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:28:17Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c98af2f3e56245ee872e4c61b972e3f22022-12-22T03:48:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016753510.1371/journal.pone.0167535Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon.Ryan T Terry-LorenzoViviana I TorresDhananjay WaghJose GalazSelene K SwansonLaurence FlorensMichael P WashburnClarissa L WaitesEckart D GundelfingerRichard J ReimerCraig C GarnerSynaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate the dynamic assembly of actin and growth factor dependent axon guidance and synaptic growth. Trio was found to interact with the C-terminal PBH 9/10 domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a domain that is also critical for its localization to the CAZ. Moreover, our data suggest that regions within the C-terminus of Trio negatively regulate its interactions with Piccolo/Bassoon. These findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis.http://europepmc.org/articles/PMC5132261?pdf=render |
| spellingShingle | Ryan T Terry-Lorenzo Viviana I Torres Dhananjay Wagh Jose Galaz Selene K Swanson Laurence Florens Michael P Washburn Clarissa L Waites Eckart D Gundelfinger Richard J Reimer Craig C Garner Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. PLoS ONE |
| title | Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. |
| title_full | Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. |
| title_fullStr | Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. |
| title_full_unstemmed | Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. |
| title_short | Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. |
| title_sort | trio a rho family gef interacts with the presynaptic active zone proteins piccolo and bassoon |
| url | http://europepmc.org/articles/PMC5132261?pdf=render |
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