Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection.
The matrix proteins of Staphylococcus aureus biofilm have not been well defined. Previous efforts to identify these proteins were performed using in vitro systems. Here we use a proteomic approach to identify biofilm matrix proteins directly from infected bone implants using a rat model of orthopedi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5679556?pdf=render |
| _version_ | 1811327761675976704 |
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| author | Mei G Lei Ravi Kr Gupta Chia Y Lee |
| author_facet | Mei G Lei Ravi Kr Gupta Chia Y Lee |
| author_sort | Mei G Lei |
| collection | DOAJ |
| description | The matrix proteins of Staphylococcus aureus biofilm have not been well defined. Previous efforts to identify these proteins were performed using in vitro systems. Here we use a proteomic approach to identify biofilm matrix proteins directly from infected bone implants using a rat model of orthopedic implant-associated S. aureus infection. Despite heavy presence of host proteins, a total of 28 and 105 S. aureus proteins were identified during acute infection and chronic infection, respectively. Our results show that biofilm matrix contains mostly intracellular cytoplasmic proteins and, to a much less extent, extracellular and cell surface-associated proteins. Significantly, leukocidins were identified in the biofilm matrix during chronic infection, suggesting S. aureus is actively attacking the host immune system even though they are protected within the biofilm. The presence of two surface-associated proteins, Ebh and SasF, in the infected bone tissue during acute infection was confirmed by immunohistochemistry. In addition, a large number of host proteins were found differentially expressed in response to S. aureus biofilm formed on bone implants. |
| first_indexed | 2024-04-13T15:13:46Z |
| format | Article |
| id | doaj.art-c98fa3e999b64c1ab0fedc64bc312d13 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T15:13:46Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c98fa3e999b64c1ab0fedc64bc312d132022-12-22T02:41:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018798110.1371/journal.pone.0187981Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection.Mei G LeiRavi Kr GuptaChia Y LeeThe matrix proteins of Staphylococcus aureus biofilm have not been well defined. Previous efforts to identify these proteins were performed using in vitro systems. Here we use a proteomic approach to identify biofilm matrix proteins directly from infected bone implants using a rat model of orthopedic implant-associated S. aureus infection. Despite heavy presence of host proteins, a total of 28 and 105 S. aureus proteins were identified during acute infection and chronic infection, respectively. Our results show that biofilm matrix contains mostly intracellular cytoplasmic proteins and, to a much less extent, extracellular and cell surface-associated proteins. Significantly, leukocidins were identified in the biofilm matrix during chronic infection, suggesting S. aureus is actively attacking the host immune system even though they are protected within the biofilm. The presence of two surface-associated proteins, Ebh and SasF, in the infected bone tissue during acute infection was confirmed by immunohistochemistry. In addition, a large number of host proteins were found differentially expressed in response to S. aureus biofilm formed on bone implants.http://europepmc.org/articles/PMC5679556?pdf=render |
| spellingShingle | Mei G Lei Ravi Kr Gupta Chia Y Lee Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection. PLoS ONE |
| title | Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection. |
| title_full | Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection. |
| title_fullStr | Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection. |
| title_full_unstemmed | Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection. |
| title_short | Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection. |
| title_sort | proteomics of staphylococcus aureus biofilm matrix in a rat model of orthopedic implant associated infection |
| url | http://europepmc.org/articles/PMC5679556?pdf=render |
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