Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains
Zika virus (ZIKV) is a mosquito-borne flavivirus that has emerged as an important human viral pathogen, causing congenital malformation including microcephaly among infants born to mothers infected with the virus during pregnancy. Phylogenetic analysis suggested that ZIKV can be classified into Afri...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-12-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/10/12/700 |
| _version_ | 1811317220799676416 |
|---|---|
| author | Lizhou Zhang Wei Ji Shuang Lyu Luhua Qiao Guangxiang Luo |
| author_facet | Lizhou Zhang Wei Ji Shuang Lyu Luhua Qiao Guangxiang Luo |
| author_sort | Lizhou Zhang |
| collection | DOAJ |
| description | Zika virus (ZIKV) is a mosquito-borne flavivirus that has emerged as an important human viral pathogen, causing congenital malformation including microcephaly among infants born to mothers infected with the virus during pregnancy. Phylogenetic analysis suggested that ZIKV can be classified into African and Asian lineages. In this study, we have developed a stable plasmid-based reverse genetic system for robust production of both ZIKV prototype African-lineage MR766 and clinical Asian-lineage FSS13025 strains using a tetracycline (Tet)-controlled gene expression vector. Transcription of the full-length ZIKV RNA is under the control of the Tet-responsive <i>P</i><sub>tight</sub> promoter at the 5′ end and an antigenomic ribozyme of hepatitis delta virus at the 3′ end. The transcription of infectious ZIKV RNA genome was efficiently induced by doxycycline. This novel ZIKV reverse genetics system will be valuable for the study of molecular viral pathogenesis of ZIKV and the development of new vaccines against ZIKV infection. |
| first_indexed | 2024-04-13T12:04:19Z |
| format | Article |
| id | doaj.art-c98fcc15333248c69163a28c06b6c230 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-13T12:04:19Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c98fcc15333248c69163a28c06b6c2302022-12-22T02:47:42ZengMDPI AGViruses1999-49152018-12-01101270010.3390/v10120700v10120700Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage StrainsLizhou Zhang0Wei Ji1Shuang Lyu2Luhua Qiao3Guangxiang Luo4Department of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADepartment of Microbiology, Peking University Health Science Center School of Basic Medical Sciences, Beijing 100191, ChinaDepartment of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADepartment of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADepartment of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USAZika virus (ZIKV) is a mosquito-borne flavivirus that has emerged as an important human viral pathogen, causing congenital malformation including microcephaly among infants born to mothers infected with the virus during pregnancy. Phylogenetic analysis suggested that ZIKV can be classified into African and Asian lineages. In this study, we have developed a stable plasmid-based reverse genetic system for robust production of both ZIKV prototype African-lineage MR766 and clinical Asian-lineage FSS13025 strains using a tetracycline (Tet)-controlled gene expression vector. Transcription of the full-length ZIKV RNA is under the control of the Tet-responsive <i>P</i><sub>tight</sub> promoter at the 5′ end and an antigenomic ribozyme of hepatitis delta virus at the 3′ end. The transcription of infectious ZIKV RNA genome was efficiently induced by doxycycline. This novel ZIKV reverse genetics system will be valuable for the study of molecular viral pathogenesis of ZIKV and the development of new vaccines against ZIKV infection.https://www.mdpi.com/1999-4915/10/12/700Zika virusreverse geneticsinfectious cDNATet-inducibleMR766FSS13025 |
| spellingShingle | Lizhou Zhang Wei Ji Shuang Lyu Luhua Qiao Guangxiang Luo Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains Viruses Zika virus reverse genetics infectious cDNA Tet-inducible MR766 FSS13025 |
| title | Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains |
| title_full | Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains |
| title_fullStr | Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains |
| title_full_unstemmed | Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains |
| title_short | Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains |
| title_sort | tet inducible production of infectious zika virus from the full length cdna clones of african and asian lineage strains |
| topic | Zika virus reverse genetics infectious cDNA Tet-inducible MR766 FSS13025 |
| url | https://www.mdpi.com/1999-4915/10/12/700 |
| work_keys_str_mv | AT lizhouzhang tetinducibleproductionofinfectiouszikavirusfromthefulllengthcdnaclonesofafricanandasianlineagestrains AT weiji tetinducibleproductionofinfectiouszikavirusfromthefulllengthcdnaclonesofafricanandasianlineagestrains AT shuanglyu tetinducibleproductionofinfectiouszikavirusfromthefulllengthcdnaclonesofafricanandasianlineagestrains AT luhuaqiao tetinducibleproductionofinfectiouszikavirusfromthefulllengthcdnaclonesofafricanandasianlineagestrains AT guangxiangluo tetinducibleproductionofinfectiouszikavirusfromthefulllengthcdnaclonesofafricanandasianlineagestrains |