Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital
Acinetobacter is an important nosocomial pathogen causing health care associated infections. It is highly antibiotic resistant gram-negative bacilli. The study was done to determine the prevalence of Acinetobacter species isolated from various clinical samples with their antibiotic susceptibility pa...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2024-03-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/prevalence-of-extended-spectrum-beta-lactamase-esbl-metallo-beta-lactamase-mbl-and-carbapenemase-producing-acinetobacter-species-isolated-from-various-clinical-samples-in-tertiary-care-hospital/ |
| _version_ | 1797257562725285888 |
|---|---|
| author | Manasi Vikas Yadav Geeta S. Karande S.N. Karande S.R. Patil Shanu Sharma |
| author_facet | Manasi Vikas Yadav Geeta S. Karande S.N. Karande S.R. Patil Shanu Sharma |
| author_sort | Manasi Vikas Yadav |
| collection | DOAJ |
| description | Acinetobacter is an important nosocomial pathogen causing health care associated infections. It is highly antibiotic resistant gram-negative bacilli. The study was done to determine the prevalence of Acinetobacter species isolated from various clinical samples with their antibiotic susceptibility pattern. To determine the antimicrobial susceptibility pattern of the isolated Acinetobacter species and also the multidrug resistant mechanisms by phenotypic characterization. The retrospective study was carried out in patients diagnosed with Acinetobacter infection in the Microbiology Department, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth, Deemed To Be University, Karad, a tertiary care hospital, including the clinical departments, during the period of two years from November 2020 till November 2022. Organism identification, biochemical test, antibiotic resistance pattern and phenotypic tests such as ESBL, MBL and Carbapenemase production were performed as per the Clinical and Laboratory Standards Institute (CLSI). The Modified Hodge test (MHT) was performed for Carbapenemase production detection in Acinetobacter species. A total of 150 Acinetobacter species were isolated from clinical samples. Acinetobacter baumannii was the most prevalent species 138 (92%), followed by Acinetobacter lwoffii 10 (7%) and Acinetobacter hemolyticus 2 (1%). The isolates showed highest resistance to Ampicillin 130 (87%) and sensitive to Colistin 113 (75.3%). Most of the isolates of of Acinetobacter baumannii showed maximum ESBL 21(14%) and MBL 75 (93%) production. Modified Hodge test showed positive results in Acinetobacter baumannii, only 11 (7%). The study showed that Acinetobacter baumannii was the most prevalent species showing drug resistance by phenotypic detection methods. At present Acinetobacter is a frequent pathogen in hospital acquired infections in critically ill patients admitted to ICU. The isolates of Acinetobacter species in our study showed resistant to most commonly used antibiotics. The study showed that ESBL production in Acinetobacter was 22 (15%) and MBL 80 (53%). Most Acinetobacter isolates were Multi Drug Resistant (MDR). MDR Acinetobacter is widely increasing due to inappropriate use of antibiotics in healthcare hospital. In our study, detection of carbapenemase by Modified Hodge test was positive in 11 (7%) isolates. |
| first_indexed | 2024-04-24T22:39:37Z |
| format | Article |
| id | doaj.art-c99240e4ac7d4dc790372a8dadec9aec |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-24T22:39:37Z |
| publishDate | 2024-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-c99240e4ac7d4dc790372a8dadec9aec2024-03-19T06:16:50ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-03-01181528541https://doi.org/10.22207/JPAM.18.1.36Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care HospitalManasi Vikas Yadavhttps://orcid.org/0000-0003-4569-9145Geeta S. Karandehttps://orcid.org/0000-0003-0750-6242S.N. Karandehttps://orcid.org/0009-0008-1011-3996S.R. Patilhttps://orcid.org/0000-0001-9112-8322Shanu Sharmahttps://orcid.org/0000-0003-4931-6682Acinetobacter is an important nosocomial pathogen causing health care associated infections. It is highly antibiotic resistant gram-negative bacilli. The study was done to determine the prevalence of Acinetobacter species isolated from various clinical samples with their antibiotic susceptibility pattern. To determine the antimicrobial susceptibility pattern of the isolated Acinetobacter species and also the multidrug resistant mechanisms by phenotypic characterization. The retrospective study was carried out in patients diagnosed with Acinetobacter infection in the Microbiology Department, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth, Deemed To Be University, Karad, a tertiary care hospital, including the clinical departments, during the period of two years from November 2020 till November 2022. Organism identification, biochemical test, antibiotic resistance pattern and phenotypic tests such as ESBL, MBL and Carbapenemase production were performed as per the Clinical and Laboratory Standards Institute (CLSI). The Modified Hodge test (MHT) was performed for Carbapenemase production detection in Acinetobacter species. A total of 150 Acinetobacter species were isolated from clinical samples. Acinetobacter baumannii was the most prevalent species 138 (92%), followed by Acinetobacter lwoffii 10 (7%) and Acinetobacter hemolyticus 2 (1%). The isolates showed highest resistance to Ampicillin 130 (87%) and sensitive to Colistin 113 (75.3%). Most of the isolates of of Acinetobacter baumannii showed maximum ESBL 21(14%) and MBL 75 (93%) production. Modified Hodge test showed positive results in Acinetobacter baumannii, only 11 (7%). The study showed that Acinetobacter baumannii was the most prevalent species showing drug resistance by phenotypic detection methods. At present Acinetobacter is a frequent pathogen in hospital acquired infections in critically ill patients admitted to ICU. The isolates of Acinetobacter species in our study showed resistant to most commonly used antibiotics. The study showed that ESBL production in Acinetobacter was 22 (15%) and MBL 80 (53%). Most Acinetobacter isolates were Multi Drug Resistant (MDR). MDR Acinetobacter is widely increasing due to inappropriate use of antibiotics in healthcare hospital. In our study, detection of carbapenemase by Modified Hodge test was positive in 11 (7%) isolates.https://microbiologyjournal.org/prevalence-of-extended-spectrum-beta-lactamase-esbl-metallo-beta-lactamase-mbl-and-carbapenemase-producing-acinetobacter-species-isolated-from-various-clinical-samples-in-tertiary-care-hospital/acinetobacterclsiicuatccesblmblmhtmdr |
| spellingShingle | Manasi Vikas Yadav Geeta S. Karande S.N. Karande S.R. Patil Shanu Sharma Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital Journal of Pure and Applied Microbiology acinetobacter clsi icu atcc esbl mbl mht mdr |
| title | Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital |
| title_full | Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital |
| title_fullStr | Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital |
| title_full_unstemmed | Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital |
| title_short | Prevalence of Extended-spectrum Beta-lactamase (ESBL), Metallo Beta-lactamase (MBL) and Carbapenemase Producing Acinetobacter Species Isolated from Various Clinical Samples in Tertiary Care Hospital |
| title_sort | prevalence of extended spectrum beta lactamase esbl metallo beta lactamase mbl and carbapenemase producing acinetobacter species isolated from various clinical samples in tertiary care hospital |
| topic | acinetobacter clsi icu atcc esbl mbl mht mdr |
| url | https://microbiologyjournal.org/prevalence-of-extended-spectrum-beta-lactamase-esbl-metallo-beta-lactamase-mbl-and-carbapenemase-producing-acinetobacter-species-isolated-from-various-clinical-samples-in-tertiary-care-hospital/ |
| work_keys_str_mv | AT manasivikasyadav prevalenceofextendedspectrumbetalactamaseesblmetallobetalactamasemblandcarbapenemaseproducingacinetobacterspeciesisolatedfromvariousclinicalsamplesintertiarycarehospital AT geetaskarande prevalenceofextendedspectrumbetalactamaseesblmetallobetalactamasemblandcarbapenemaseproducingacinetobacterspeciesisolatedfromvariousclinicalsamplesintertiarycarehospital AT snkarande prevalenceofextendedspectrumbetalactamaseesblmetallobetalactamasemblandcarbapenemaseproducingacinetobacterspeciesisolatedfromvariousclinicalsamplesintertiarycarehospital AT srpatil prevalenceofextendedspectrumbetalactamaseesblmetallobetalactamasemblandcarbapenemaseproducingacinetobacterspeciesisolatedfromvariousclinicalsamplesintertiarycarehospital AT shanusharma prevalenceofextendedspectrumbetalactamaseesblmetallobetalactamasemblandcarbapenemaseproducingacinetobacterspeciesisolatedfromvariousclinicalsamplesintertiarycarehospital |