Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts
The use of enzymes in diverse industries has increased substantially over past decades, creating a well-established and growing global market. Currently, the use of enzymes that work better at ambient or lower temperatures in order to decrease the temperatures of production processes is desirable. T...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-12-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmolb.2017.00086/full |
| _version_ | 1819058657283801088 |
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| author | Yassef Yuivar Salvador Barahona Jennifer Alcaíno Víctor Cifuentes Marcelo Baeza |
| author_facet | Yassef Yuivar Salvador Barahona Jennifer Alcaíno Víctor Cifuentes Marcelo Baeza |
| author_sort | Yassef Yuivar |
| collection | DOAJ |
| description | The use of enzymes in diverse industries has increased substantially over past decades, creating a well-established and growing global market. Currently, the use of enzymes that work better at ambient or lower temperatures in order to decrease the temperatures of production processes is desirable. There is thus a continuous search for enzymes in cold environments, especially from microbial sources, with amylases, proteases, lipases and, cellulases being the most studied. Other enzymes, such as glucose oxidase (GOD), invertase (Inv), and alkaline phosphatase (ALP), also have a high potential for application, but have been much less studied in microorganisms living in cold-environments. In this work, secretion of these three enzymes by Antarctic yeast species was analyzed, and five, three, and five species were found to produce extracellular GOD, Inv, and ALP, respectively. The major producers of GOD, Inv, and ALP were Goffeauzyma gastrica, Wickerhamomyces anomalus, and Dioszegia sp., respectively, from which the enzymes were purified and characterized. Contrary to what was expected, the highest GOD and Inv activities were found at 64°C and 60°C, respectively, and at 47°C for ALP. However, the three enzymes maintained a significant percentage of activity at lower temperatures, especially ALP that kept a 67 and 43% of activity at 10°C and 4°C, respectively. |
| first_indexed | 2024-12-21T13:58:41Z |
| format | Article |
| id | doaj.art-c99fdfc53d5140b3a41c809e14f4585c |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-21T13:58:41Z |
| publishDate | 2017-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-c99fdfc53d5140b3a41c809e14f4585c2022-12-21T19:01:26ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2017-12-01410.3389/fmolb.2017.00086311608Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic YeastsYassef YuivarSalvador BarahonaJennifer AlcaínoVíctor CifuentesMarcelo BaezaThe use of enzymes in diverse industries has increased substantially over past decades, creating a well-established and growing global market. Currently, the use of enzymes that work better at ambient or lower temperatures in order to decrease the temperatures of production processes is desirable. There is thus a continuous search for enzymes in cold environments, especially from microbial sources, with amylases, proteases, lipases and, cellulases being the most studied. Other enzymes, such as glucose oxidase (GOD), invertase (Inv), and alkaline phosphatase (ALP), also have a high potential for application, but have been much less studied in microorganisms living in cold-environments. In this work, secretion of these three enzymes by Antarctic yeast species was analyzed, and five, three, and five species were found to produce extracellular GOD, Inv, and ALP, respectively. The major producers of GOD, Inv, and ALP were Goffeauzyma gastrica, Wickerhamomyces anomalus, and Dioszegia sp., respectively, from which the enzymes were purified and characterized. Contrary to what was expected, the highest GOD and Inv activities were found at 64°C and 60°C, respectively, and at 47°C for ALP. However, the three enzymes maintained a significant percentage of activity at lower temperatures, especially ALP that kept a 67 and 43% of activity at 10°C and 4°C, respectively.http://journal.frontiersin.org/article/10.3389/fmolb.2017.00086/fullantarctic yeastsglucose oxidaseinvertasealkaline phosphataseyeast secreted enzymes |
| spellingShingle | Yassef Yuivar Salvador Barahona Jennifer Alcaíno Víctor Cifuentes Marcelo Baeza Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts Frontiers in Molecular Biosciences antarctic yeasts glucose oxidase invertase alkaline phosphatase yeast secreted enzymes |
| title | Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts |
| title_full | Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts |
| title_fullStr | Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts |
| title_full_unstemmed | Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts |
| title_short | Biochemical and Thermodynamical Characterization of Glucose Oxidase, Invertase, and Alkaline Phosphatase Secreted by Antarctic Yeasts |
| title_sort | biochemical and thermodynamical characterization of glucose oxidase invertase and alkaline phosphatase secreted by antarctic yeasts |
| topic | antarctic yeasts glucose oxidase invertase alkaline phosphatase yeast secreted enzymes |
| url | http://journal.frontiersin.org/article/10.3389/fmolb.2017.00086/full |
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