Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS
Seminal plasma (SP) mirrors the local pathophysiology of the male reproductive system and represents a non-invasive fluid for the study of infertility. Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) provides a high-throughput platform to rapidly extrapola...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10786 |
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| author | Serena Correnti Mariaimmacolata Preianò Pierpaolo Murfone Annalisa Fregola Massimo Bitonti Rocco Savino Rosa Terracciano |
| author_facet | Serena Correnti Mariaimmacolata Preianò Pierpaolo Murfone Annalisa Fregola Massimo Bitonti Rocco Savino Rosa Terracciano |
| author_sort | Serena Correnti |
| collection | DOAJ |
| description | Seminal plasma (SP) mirrors the local pathophysiology of the male reproductive system and represents a non-invasive fluid for the study of infertility. Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) provides a high-throughput platform to rapidly extrapolate the diagnostic profiles of information-rich patterns. In this study, dispersive solid phase extraction (<i>d</i>-SPE) combined with MALDI-TOF-MS was applied for the first time to the human SP, with the aim of revealing a diagnostic signature for male infertility. Commercially available octadecyl (C<sub>18</sub>)-, octyl (C<sub>8</sub>)-bonded silica sorbents and hexagonal mesoporous silica (HMS) were tested and the robustness of MALDI-TOF peptide profiling was evaluated. Best performances were obtained for C<sub>18</sub>-bonded silica with the highest detection of peaks and the lowest variation of spectral features. To assess the diagnostic potential of the method, C<sub>18</sub>-bonded silica <i>d</i>-SPE and MALDI-TOF-MS were used to generate enriched endogenous peptide profiles of SP from 15 fertile and 15 non-fertile donors. Principal component analysis (PCA) successfully separated fertile from non-fertile men into two different clusters. An array of seven semenogelin-derived peptides was found to distinguish the two groups, with high statistical significance. These findings, while providing a rapid and convenient route to selectively enrich native components of SP peptidome, strongly reinforce the prominent role of semenogelins in male infertility. |
| first_indexed | 2024-03-09T23:44:11Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:44:11Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c9a28fff372b4eafbe486419a1e365302023-11-23T16:47:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181078610.3390/ijms231810786Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MSSerena Correnti0Mariaimmacolata Preianò1Pierpaolo Murfone2Annalisa Fregola3Massimo Bitonti4Rocco Savino5Rosa Terracciano6Department of Health Sciences, Magna Græcia University, 88100 Catanzaro, ItalyDepartment of Health Sciences, Magna Græcia University, 88100 Catanzaro, ItalyDepartment of Experimental and Clinical Medicine, Magna Græcia University, 88100 Catanzaro, ItalyBiopepticom S.R.L., 88100 Catanzaro, ItalyBiopepticom S.R.L., 88100 Catanzaro, ItalyDepartment of Medical and Surgical Sciences, Magna Græcia University, 88100 Catanzaro, ItalyDepartment of Experimental and Clinical Medicine, Magna Græcia University, 88100 Catanzaro, ItalySeminal plasma (SP) mirrors the local pathophysiology of the male reproductive system and represents a non-invasive fluid for the study of infertility. Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) provides a high-throughput platform to rapidly extrapolate the diagnostic profiles of information-rich patterns. In this study, dispersive solid phase extraction (<i>d</i>-SPE) combined with MALDI-TOF-MS was applied for the first time to the human SP, with the aim of revealing a diagnostic signature for male infertility. Commercially available octadecyl (C<sub>18</sub>)-, octyl (C<sub>8</sub>)-bonded silica sorbents and hexagonal mesoporous silica (HMS) were tested and the robustness of MALDI-TOF peptide profiling was evaluated. Best performances were obtained for C<sub>18</sub>-bonded silica with the highest detection of peaks and the lowest variation of spectral features. To assess the diagnostic potential of the method, C<sub>18</sub>-bonded silica <i>d</i>-SPE and MALDI-TOF-MS were used to generate enriched endogenous peptide profiles of SP from 15 fertile and 15 non-fertile donors. Principal component analysis (PCA) successfully separated fertile from non-fertile men into two different clusters. An array of seven semenogelin-derived peptides was found to distinguish the two groups, with high statistical significance. These findings, while providing a rapid and convenient route to selectively enrich native components of SP peptidome, strongly reinforce the prominent role of semenogelins in male infertility.https://www.mdpi.com/1422-0067/23/18/10786MALDI-TOF/TOFmass spectrometryproteomeseminal plasmamale infertilityseminal fluid |
| spellingShingle | Serena Correnti Mariaimmacolata Preianò Pierpaolo Murfone Annalisa Fregola Massimo Bitonti Rocco Savino Rosa Terracciano Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS International Journal of Molecular Sciences MALDI-TOF/TOF mass spectrometry proteome seminal plasma male infertility seminal fluid |
| title | Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS |
| title_full | Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS |
| title_fullStr | Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS |
| title_full_unstemmed | Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS |
| title_short | Revealing the Hidden Diagnostic Clues of Male Infertility from Human Seminal Plasma by Dispersive Solid Phase Extraction and MALDI-TOF MS |
| title_sort | revealing the hidden diagnostic clues of male infertility from human seminal plasma by dispersive solid phase extraction and maldi tof ms |
| topic | MALDI-TOF/TOF mass spectrometry proteome seminal plasma male infertility seminal fluid |
| url | https://www.mdpi.com/1422-0067/23/18/10786 |
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