Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study

Tulsi (Ocimum sanctum) leaf extract was used to manufacture stable silver nanoparticles. The synthesized AgNPs were characterized by EDX (Energy dispersive X-ray Spectrometry), TEM, and DLR to confirm the size of particles which varied widely within 100 nm and pure. In-vitro analysis was carried out...

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Main Authors: Ratan Das, Prahlad Deb, Himanshu Pandey, Perugu Shyam, Devendra Singh
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Journal of Agriculture and Food Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666154322001235
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author Ratan Das
Prahlad Deb
Himanshu Pandey
Perugu Shyam
Devendra Singh
author_facet Ratan Das
Prahlad Deb
Himanshu Pandey
Perugu Shyam
Devendra Singh
author_sort Ratan Das
collection DOAJ
description Tulsi (Ocimum sanctum) leaf extract was used to manufacture stable silver nanoparticles. The synthesized AgNPs were characterized by EDX (Energy dispersive X-ray Spectrometry), TEM, and DLR to confirm the size of particles which varied widely within 100 nm and pure. In-vitro analysis was carried out to find the best concentration of AgNPs against Alternaria porri, which showed various levels of inhibition. Maximum inhibition percentage was recorded at 150ppm (91.71%) followed by 125 (91.24%) and 100ppm (87.56%). Similarly, AgNPs was also evaluated against Alternaria porri in natural epiphytic condition. From the result of the field experiment, it has been observed that AgNPs were capable enough to manage the purple blotch disease. The maximum inhibition percentage of purple blotch disease was observed at 100ppm (80.93%), followed by 75ppm (77.17%) under field conditions.
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spelling doaj.art-c9a3162ed0974766952ed9c508689d702022-12-22T03:52:56ZengElsevierJournal of Agriculture and Food Research2666-15432022-12-0110100390Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic studyRatan Das0Prahlad Deb1Himanshu Pandey2Perugu Shyam3Devendra Singh4Krishi Vigyan Kendra, Chantail, Unakoti, Tripura, 799279, IndiaInstitute of Agriculture, Visva-Bharati, Sriniketan, WB, 731 236, IndiaDr. YSP UHF Nauni, Solan, 173230, Himachal Pradesh, IndiaNational Institute of Technology Warangal, Kazipet, Warangal, PIN-506004, Telangana, India; Corresponding author.National Institute of Technology Warangal, Kazipet, Warangal, PIN-506004, Telangana, India; Corresponding author.Tulsi (Ocimum sanctum) leaf extract was used to manufacture stable silver nanoparticles. The synthesized AgNPs were characterized by EDX (Energy dispersive X-ray Spectrometry), TEM, and DLR to confirm the size of particles which varied widely within 100 nm and pure. In-vitro analysis was carried out to find the best concentration of AgNPs against Alternaria porri, which showed various levels of inhibition. Maximum inhibition percentage was recorded at 150ppm (91.71%) followed by 125 (91.24%) and 100ppm (87.56%). Similarly, AgNPs was also evaluated against Alternaria porri in natural epiphytic condition. From the result of the field experiment, it has been observed that AgNPs were capable enough to manage the purple blotch disease. The maximum inhibition percentage of purple blotch disease was observed at 100ppm (80.93%), followed by 75ppm (77.17%) under field conditions.http://www.sciencedirect.com/science/article/pii/S2666154322001235Alternaria porriAgNPsOnionPurple blotch
spellingShingle Ratan Das
Prahlad Deb
Himanshu Pandey
Perugu Shyam
Devendra Singh
Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study
Journal of Agriculture and Food Research
Alternaria porri
AgNPs
Onion
Purple blotch
title Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study
title_full Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study
title_fullStr Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study
title_full_unstemmed Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study
title_short Botanical synthesis of silver nanoparticles (AgNPs) and its antifungal effect against Alternaria porri causing purple blotch of onion: An in vitro and natural epiphytic study
title_sort botanical synthesis of silver nanoparticles agnps and its antifungal effect against alternaria porri causing purple blotch of onion an in vitro and natural epiphytic study
topic Alternaria porri
AgNPs
Onion
Purple blotch
url http://www.sciencedirect.com/science/article/pii/S2666154322001235
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