Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis
BackgroundAllergic rhinoconjunctivitis (ARC) and allergic rhinitis (AR) are prevalent allergic diseases. People are becoming increasingly aware of the impact of microbial disorders on host immunity and allergic diseases. Studies have demonstrated an association between allergic diseases and the micr...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1079154/full |
| _version_ | 1797867138944335872 |
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| author | Yuan Wang Yuan Wang Yuan Wang Xuan Li Xuan Li Xuan Li Shuntong Gu Shuntong Gu Junhong Fu |
| author_facet | Yuan Wang Yuan Wang Yuan Wang Xuan Li Xuan Li Xuan Li Shuntong Gu Shuntong Gu Junhong Fu |
| author_sort | Yuan Wang |
| collection | DOAJ |
| description | BackgroundAllergic rhinoconjunctivitis (ARC) and allergic rhinitis (AR) are prevalent allergic diseases. People are becoming increasingly aware of the impact of microbial disorders on host immunity and allergic diseases. Studies have demonstrated an association between allergic diseases and the microbiome, but much remains unknown. We assessed changes in the conjunctival microbiome and nasal microbiome in patients with ARC or AR.MethodsConjunctival swabs and nasal swabs were collected from each participant for 16S rRNA amplicon sequencing. Bacterial communities were analyzed.ResultsForty patients with ARC, 20 patients suffering from AR, and 34 healthy controls (HCs) were recruited. This study found the abundance of conjunctival microbiome in patients with ARC or AR was significantly lower than that in HCs. The diversity of conjunctival microbiome in patients with AR was significantly lower than those in the other two groups. There is no significant difference in abundance of nasal microbiome between the three groups. The diversities of nasal microbiome in patients with ARC or AR were significantly lower than that in HCs. We found significant differences in microbiota compositions in patients with ARC or AR compared with those in HCs. However, no significant difference in microbiota compositions was found between patients with ARC and patients with AR. Microbiome functions in the ARC group and AR group were also altered compared with HCs.ConclusionsWe revealed changes in the composition and function of the conjunctival microbiome and nasal microbiome of patients with ARC or AR, which suggests that there is a relationship between allergic conditions and the local microbiome. |
| first_indexed | 2024-04-09T23:36:36Z |
| format | Article |
| id | doaj.art-c9a3e4d20076448183dc226ff420f549 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T23:36:36Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-c9a3e4d20076448183dc226ff420f5492023-03-20T11:08:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-03-011410.3389/fimmu.2023.10791541079154Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitisYuan Wang0Yuan Wang1Yuan Wang2Xuan Li3Xuan Li4Xuan Li5Shuntong Gu6Shuntong Gu7Junhong Fu8Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, ChinaTianjin Eye Hospital, Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin Eye Institute, Tianjin, ChinaDepartment of Otolaryngology-Head & Neck Surgery, Tianjin TEDA Hospital, Tianjin, ChinaClinical College of Ophthalmology, Tianjin Medical University, Tianjin, ChinaTianjin Eye Hospital, Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin Eye Institute, Tianjin, ChinaNankai University Affiliated Eye Hospital, Nankai University, Tianjin, ChinaDepartment of General Surgery, Tianjin Fifth Central Hospital, Tianjin, ChinaDepartment of Vascular Surgery, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Ophthalmology, Tianjin TEDA Hospital, Tianjin, ChinaBackgroundAllergic rhinoconjunctivitis (ARC) and allergic rhinitis (AR) are prevalent allergic diseases. People are becoming increasingly aware of the impact of microbial disorders on host immunity and allergic diseases. Studies have demonstrated an association between allergic diseases and the microbiome, but much remains unknown. We assessed changes in the conjunctival microbiome and nasal microbiome in patients with ARC or AR.MethodsConjunctival swabs and nasal swabs were collected from each participant for 16S rRNA amplicon sequencing. Bacterial communities were analyzed.ResultsForty patients with ARC, 20 patients suffering from AR, and 34 healthy controls (HCs) were recruited. This study found the abundance of conjunctival microbiome in patients with ARC or AR was significantly lower than that in HCs. The diversity of conjunctival microbiome in patients with AR was significantly lower than those in the other two groups. There is no significant difference in abundance of nasal microbiome between the three groups. The diversities of nasal microbiome in patients with ARC or AR were significantly lower than that in HCs. We found significant differences in microbiota compositions in patients with ARC or AR compared with those in HCs. However, no significant difference in microbiota compositions was found between patients with ARC and patients with AR. Microbiome functions in the ARC group and AR group were also altered compared with HCs.ConclusionsWe revealed changes in the composition and function of the conjunctival microbiome and nasal microbiome of patients with ARC or AR, which suggests that there is a relationship between allergic conditions and the local microbiome.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1079154/fullallergic rhinoconjunctivitisallergic rhinitismicrobiomeconjunctivalnasal16s rRNA amplicon sequencing |
| spellingShingle | Yuan Wang Yuan Wang Yuan Wang Xuan Li Xuan Li Xuan Li Shuntong Gu Shuntong Gu Junhong Fu Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis Frontiers in Immunology allergic rhinoconjunctivitis allergic rhinitis microbiome conjunctival nasal 16s rRNA amplicon sequencing |
| title | Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis |
| title_full | Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis |
| title_fullStr | Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis |
| title_full_unstemmed | Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis |
| title_short | Characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis |
| title_sort | characterization of dysbiosis of the conjunctival microbiome and nasal microbiome associated with allergic rhinoconjunctivitis and allergic rhinitis |
| topic | allergic rhinoconjunctivitis allergic rhinitis microbiome conjunctival nasal 16s rRNA amplicon sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1079154/full |
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