Development of a New Model of Humeral Hemiarthroplasty in Rats
Purpose In vivo models are anatomically comparable to humans allowing to reproduce the patterns and progression of the disease and giving the opportunity to study the symptoms and responses to new treatments and materials. This study aimed to establish a valid and cost-effective in vivo rat model to...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2022.2162636 |
| _version_ | 1797684499541131264 |
|---|---|
| author | Efi Kazum Eran Maman Zachary T. Sharfman Reut Wengier Osnat Sher Amal Khoury Ofir Chechik Oleg Dolkart |
| author_facet | Efi Kazum Eran Maman Zachary T. Sharfman Reut Wengier Osnat Sher Amal Khoury Ofir Chechik Oleg Dolkart |
| author_sort | Efi Kazum |
| collection | DOAJ |
| description | Purpose In vivo models are anatomically comparable to humans allowing to reproduce the patterns and progression of the disease and giving the opportunity to study the symptoms and responses to new treatments and materials. This study aimed to establish a valid and cost-effective in vivo rat model to assess the effects of implanted shoulder hemiarthroplasty materials on glenoid articular cartilage wear. Methods Eight adult male Wistar rats underwent right shoulder hemi-arthroplasty. A stainless steel metal bearing was used as a shoulder joint prosthesis. X-rays were performed one week after surgery to verify correct implant position. Additional X-rays were performed 30 and 60 days post-implantation. Animals were sacrificed 24 weeks after implantation. All specimens were evaluated with micro-CT for cartilage and bone wear characteristics as well as histologically for signs of osteoarthritis. Samples were compared to the non-operated shoulders. Results All animals recovered and resumed normal cage activity. All X-rays demonstrated correct implant positioning except for one in which the implant was displaced. Histologic evaluation demonstrated arthritic changes in the implanted shoulder. Decreased Trabecular thickness and Trabecular Spacing were documented among the implanted parties (p < .05). Bone Mineral Density and Tissue Mineral Density were reduced in the operated shoulder although not significantly (p = .07). Conclusions This study demonstrated significant glenoid cartilage wearing in the operated shoulder. Furthermore, the presence of an intra-articular hemiarthroplasty implant diminished underlying glenoid bone quality. This novel, in vivo-model will enable researchers to test implant materials and their effects on cartilage and bone tissue in a cost-effective reproducible rat model. |
| first_indexed | 2024-03-12T00:30:32Z |
| format | Article |
| id | doaj.art-c9a40b7c258f493fb851963358135a16 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:30:32Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-c9a40b7c258f493fb851963358135a162023-09-15T10:21:30ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532023-12-0136110.1080/08941939.2022.21626362162636Development of a New Model of Humeral Hemiarthroplasty in RatsEfi Kazum0Eran Maman1Zachary T. Sharfman2Reut Wengier3Osnat Sher4Amal Khoury5Ofir Chechik6Oleg Dolkart7Division of Orthopaedic Surgery, Shoulder Unit, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv UniversityDivision of Orthopaedic Surgery, Shoulder Unit, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv UniversityDepartment of Orthopaedic Surgery, Montefiore Medical Center, the University Hospital for Albert Einstein College of MedicineDepartment of Orthopedic Surgery, Assuta Ashdod Medical Center and the Ben-Gurion University of the NegevPathology Institute, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv UniversityDivision of Orthopaedic Surgery, Shoulder Unit, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv UniversityDivision of Orthopaedic Surgery, Shoulder Unit, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv UniversityDepartment of Orthopedic Surgery, Assuta Ashdod Medical Center and the Ben-Gurion University of the NegevPurpose In vivo models are anatomically comparable to humans allowing to reproduce the patterns and progression of the disease and giving the opportunity to study the symptoms and responses to new treatments and materials. This study aimed to establish a valid and cost-effective in vivo rat model to assess the effects of implanted shoulder hemiarthroplasty materials on glenoid articular cartilage wear. Methods Eight adult male Wistar rats underwent right shoulder hemi-arthroplasty. A stainless steel metal bearing was used as a shoulder joint prosthesis. X-rays were performed one week after surgery to verify correct implant position. Additional X-rays were performed 30 and 60 days post-implantation. Animals were sacrificed 24 weeks after implantation. All specimens were evaluated with micro-CT for cartilage and bone wear characteristics as well as histologically for signs of osteoarthritis. Samples were compared to the non-operated shoulders. Results All animals recovered and resumed normal cage activity. All X-rays demonstrated correct implant positioning except for one in which the implant was displaced. Histologic evaluation demonstrated arthritic changes in the implanted shoulder. Decreased Trabecular thickness and Trabecular Spacing were documented among the implanted parties (p < .05). Bone Mineral Density and Tissue Mineral Density were reduced in the operated shoulder although not significantly (p = .07). Conclusions This study demonstrated significant glenoid cartilage wearing in the operated shoulder. Furthermore, the presence of an intra-articular hemiarthroplasty implant diminished underlying glenoid bone quality. This novel, in vivo-model will enable researchers to test implant materials and their effects on cartilage and bone tissue in a cost-effective reproducible rat model.http://dx.doi.org/10.1080/08941939.2022.2162636shoulder hemiarthroplastyanimal modelsglenohumeral osteoarthritisglenoid cartilage |
| spellingShingle | Efi Kazum Eran Maman Zachary T. Sharfman Reut Wengier Osnat Sher Amal Khoury Ofir Chechik Oleg Dolkart Development of a New Model of Humeral Hemiarthroplasty in Rats Journal of Investigative Surgery shoulder hemiarthroplasty animal models glenohumeral osteoarthritis glenoid cartilage |
| title | Development of a New Model of Humeral Hemiarthroplasty in Rats |
| title_full | Development of a New Model of Humeral Hemiarthroplasty in Rats |
| title_fullStr | Development of a New Model of Humeral Hemiarthroplasty in Rats |
| title_full_unstemmed | Development of a New Model of Humeral Hemiarthroplasty in Rats |
| title_short | Development of a New Model of Humeral Hemiarthroplasty in Rats |
| title_sort | development of a new model of humeral hemiarthroplasty in rats |
| topic | shoulder hemiarthroplasty animal models glenohumeral osteoarthritis glenoid cartilage |
| url | http://dx.doi.org/10.1080/08941939.2022.2162636 |
| work_keys_str_mv | AT efikazum developmentofanewmodelofhumeralhemiarthroplastyinrats AT eranmaman developmentofanewmodelofhumeralhemiarthroplastyinrats AT zacharytsharfman developmentofanewmodelofhumeralhemiarthroplastyinrats AT reutwengier developmentofanewmodelofhumeralhemiarthroplastyinrats AT osnatsher developmentofanewmodelofhumeralhemiarthroplastyinrats AT amalkhoury developmentofanewmodelofhumeralhemiarthroplastyinrats AT ofirchechik developmentofanewmodelofhumeralhemiarthroplastyinrats AT olegdolkart developmentofanewmodelofhumeralhemiarthroplastyinrats |