Isotype specific immune responses in murine experimental toxocariasis
In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. can...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2001-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400018 |
| _version_ | 1797718107671756800 |
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| author | C Cuéllar S Fenoy C del Águila JL Guillén |
| author_facet | C Cuéllar S Fenoy C del Águila JL Guillén |
| author_sort | C Cuéllar |
| collection | DOAJ |
| description | In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, IgM, IgG, IgA, IgG1, IgG2a and IgG3 were tested by ELISA. The dynamics of the specific immunoglobulins (IgG+IgM) production showed a contrasting profile regarding the murine strain. Conversely to the results obtained with the IgM isotype, the IgG antibody class showed similar patterns to those obtained with IgG+IgM antibodies, only in the case of the BALB/c strain, being different and much higher than the obtained with IgG+IgM antibodies, when the C3H murine strain was used. The antibodies IgG+IgM tested in BALB/c and C57BL/10 were both of the IgM and IgG isotypes. Conversely, in the C3H strain only IgG specific antibody levels were detected. The IgG1 subclass responses showed a similar profile in the three murine strains studied, with high values in BALB/c, as in the case of the IgG responses. |
| first_indexed | 2024-03-12T08:47:11Z |
| format | Article |
| id | doaj.art-c9a5609012744ef287d74b4e74bf16bb |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:47:11Z |
| publishDate | 2001-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c9a5609012744ef287d74b4e74bf16bb2023-09-02T16:26:10ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602001-05-0196454955310.1590/S0074-02762001000400018Isotype specific immune responses in murine experimental toxocariasisC CuéllarS FenoyC del ÁguilaJL GuillénIn this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, IgM, IgG, IgA, IgG1, IgG2a and IgG3 were tested by ELISA. The dynamics of the specific immunoglobulins (IgG+IgM) production showed a contrasting profile regarding the murine strain. Conversely to the results obtained with the IgM isotype, the IgG antibody class showed similar patterns to those obtained with IgG+IgM antibodies, only in the case of the BALB/c strain, being different and much higher than the obtained with IgG+IgM antibodies, when the C3H murine strain was used. The antibodies IgG+IgM tested in BALB/c and C57BL/10 were both of the IgM and IgG isotypes. Conversely, in the C3H strain only IgG specific antibody levels were detected. The IgG1 subclass responses showed a similar profile in the three murine strains studied, with high values in BALB/c, as in the case of the IgG responses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400018Toxocara canisBALB/cC57BL/10C3HELISAimmunoglobulins |
| spellingShingle | C Cuéllar S Fenoy C del Águila JL Guillén Isotype specific immune responses in murine experimental toxocariasis Memorias do Instituto Oswaldo Cruz Toxocara canis BALB/c C57BL/10 C3H ELISA immunoglobulins |
| title | Isotype specific immune responses in murine experimental toxocariasis |
| title_full | Isotype specific immune responses in murine experimental toxocariasis |
| title_fullStr | Isotype specific immune responses in murine experimental toxocariasis |
| title_full_unstemmed | Isotype specific immune responses in murine experimental toxocariasis |
| title_short | Isotype specific immune responses in murine experimental toxocariasis |
| title_sort | isotype specific immune responses in murine experimental toxocariasis |
| topic | Toxocara canis BALB/c C57BL/10 C3H ELISA immunoglobulins |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400018 |
| work_keys_str_mv | AT ccuellar isotypespecificimmuneresponsesinmurineexperimentaltoxocariasis AT sfenoy isotypespecificimmuneresponsesinmurineexperimentaltoxocariasis AT cdelaguila isotypespecificimmuneresponsesinmurineexperimentaltoxocariasis AT jlguillen isotypespecificimmuneresponsesinmurineexperimentaltoxocariasis |