Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears
Abstract Despite orientationally variant tears of the meniscus, suture repair is the current clinical gold treatment. However, inaccessible tears in company with re-tears susceptibility remain unresolved. To extend meniscal repair tools from the perspective of adhesion and regeneration, we design a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-47029-6 |
| _version_ | 1797233487836610560 |
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| author | Xihao Pan Rui Li Wenyue Li Wei Sun Yiyang Yan Xiaochen Xiang Jinghua Fang Youguo Liao Chang Xie Xiaozhao Wang Youzhi Cai Xudong Yao Hongwei Ouyang |
| author_facet | Xihao Pan Rui Li Wenyue Li Wei Sun Yiyang Yan Xiaochen Xiang Jinghua Fang Youguo Liao Chang Xie Xiaozhao Wang Youzhi Cai Xudong Yao Hongwei Ouyang |
| author_sort | Xihao Pan |
| collection | DOAJ |
| description | Abstract Despite orientationally variant tears of the meniscus, suture repair is the current clinical gold treatment. However, inaccessible tears in company with re-tears susceptibility remain unresolved. To extend meniscal repair tools from the perspective of adhesion and regeneration, we design a dual functional biologic-released bioadhesive (S-PIL10) comprised of methacrylated silk fibroin crosslinked with phenylboronic acid-ionic liquid loading with growth factor TGF-β1, which integrates chemo-mechanical restoration with inner meniscal regeneration. Supramolecular interactions of β-sheets and hydrogen bonds richened by phenylboronic acid-ionic liquid (PIL) result in enhanced wet adhesion, swelling resistance, and anti-fatigue capabilities, compared to neat silk fibroin gel. Besides, elimination of reactive oxygen species (ROS) by S-PIL10 further fortifies localized meniscus tear repair by affecting inflammatory microenvironment with dynamic borate ester bonds, and S-PIL10 continuously releases TGF-β1 for cell recruitment and bridging of defect edge. In vivo rabbit models functionally evidence the seamless and dense reconstruction of torn meniscus, verifying that the concept of meniscus adhesive is feasible and providing a promising revolutionary strategy for preclinical research to repair meniscus tears. |
| first_indexed | 2024-04-24T16:16:57Z |
| format | Article |
| id | doaj.art-c9a5733ff9244961a74f23f5b8ed2666 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T16:16:57Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c9a5733ff9244961a74f23f5b8ed26662024-03-31T11:24:39ZengNature PortfolioNature Communications2041-17232024-03-0115111210.1038/s41467-024-47029-6Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tearsXihao Pan0Rui Li1Wenyue Li2Wei Sun3Yiyang Yan4Xiaochen Xiang5Jinghua Fang6Youguo Liao7Chang Xie8Xiaozhao Wang9Youzhi Cai10Xudong Yao11Hongwei Ouyang12Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineZhejiang University-University of Edinburgh Institute, Zhejiang University School of MedicineOrthopedics Research Institute, Zhejiang UniversityDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineSports Medical Center, the First Affiliated Hospital, School of Medicine, Zhejiang UniversityThe Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of MedicineDepartment of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of MedicineAbstract Despite orientationally variant tears of the meniscus, suture repair is the current clinical gold treatment. However, inaccessible tears in company with re-tears susceptibility remain unresolved. To extend meniscal repair tools from the perspective of adhesion and regeneration, we design a dual functional biologic-released bioadhesive (S-PIL10) comprised of methacrylated silk fibroin crosslinked with phenylboronic acid-ionic liquid loading with growth factor TGF-β1, which integrates chemo-mechanical restoration with inner meniscal regeneration. Supramolecular interactions of β-sheets and hydrogen bonds richened by phenylboronic acid-ionic liquid (PIL) result in enhanced wet adhesion, swelling resistance, and anti-fatigue capabilities, compared to neat silk fibroin gel. Besides, elimination of reactive oxygen species (ROS) by S-PIL10 further fortifies localized meniscus tear repair by affecting inflammatory microenvironment with dynamic borate ester bonds, and S-PIL10 continuously releases TGF-β1 for cell recruitment and bridging of defect edge. In vivo rabbit models functionally evidence the seamless and dense reconstruction of torn meniscus, verifying that the concept of meniscus adhesive is feasible and providing a promising revolutionary strategy for preclinical research to repair meniscus tears.https://doi.org/10.1038/s41467-024-47029-6 |
| spellingShingle | Xihao Pan Rui Li Wenyue Li Wei Sun Yiyang Yan Xiaochen Xiang Jinghua Fang Youguo Liao Chang Xie Xiaozhao Wang Youzhi Cai Xudong Yao Hongwei Ouyang Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears Nature Communications |
| title | Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears |
| title_full | Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears |
| title_fullStr | Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears |
| title_full_unstemmed | Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears |
| title_short | Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears |
| title_sort | silk fibroin hydrogel adhesive enables sealed tight reconstruction of meniscus tears |
| url | https://doi.org/10.1038/s41467-024-47029-6 |
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