Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites
<i>Pantoea ananatis,</i> a gram-negative bacterium belonging to the <i>Erwiniaceae</i> family, is a well-known phytopathogen isolated from many ecological niches and plant hosts. However, this bacterium also provides us with various beneficial characteristics, such as the gro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1133 |
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| author | Yoshihiro Usuda Yousuke Nishio Gen Nonaka Yoshihiko Hara |
| author_facet | Yoshihiro Usuda Yousuke Nishio Gen Nonaka Yoshihiko Hara |
| author_sort | Yoshihiro Usuda |
| collection | DOAJ |
| description | <i>Pantoea ananatis,</i> a gram-negative bacterium belonging to the <i>Erwiniaceae</i> family, is a well-known phytopathogen isolated from many ecological niches and plant hosts. However, this bacterium also provides us with various beneficial characteristics, such as the growth promotion of their host plants and increased crop yield. Some isolated non-pathogenic strains are promising for the microbial production of useful substances. <i>P. ananatis</i> AJ13355 was isolated as an acidophilic bacterium and was used as an excellent host to produce <span style="font-variant: small-caps;">L</span>-glutamic acid under acidic conditions. The genome sequence of <i>P. ananatis</i> AJ13355 was determined, and specific genome-engineering technologies were developed. As a result, <i>P. ananatis</i> was successfully used to construct a bacterial strain that produces cysteine, a sulfur-containing amino acid that has been difficult to produce through fermentation because of complex regulation. Furthermore, by heterologous expression including plant-derived genes, construction of a strain that produces isoprenoids such as isoprene and linalool as secondary metabolites was achieved. <i>P. ananatis</i> is shown to be a useful host for the production of secondary metabolites, as well as amino acids, and is expected to be used as a platform for microbial production of bioactive substances, aromatic substances, and other high-value-added substances of plant origin in the future. |
| first_indexed | 2024-03-09T23:00:25Z |
| format | Article |
| id | doaj.art-c9abcbee13c84104a3f24f2be3b51083 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:00:25Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c9abcbee13c84104a3f24f2be3b510832023-11-23T18:03:32ZengMDPI AGMicroorganisms2076-26072022-05-01106113310.3390/microorganisms10061133Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary MetabolitesYoshihiro Usuda0Yousuke Nishio1Gen Nonaka2Yoshihiko Hara3Research and Business Planning Department, Ajinomoto Co., Inc., Tokyo 104-8315, JapanResearch Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki 210-8681, JapanAjinomoto-Genetika Research Institute, Moscow 117545, RussiaResearch Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki 210-8681, Japan<i>Pantoea ananatis,</i> a gram-negative bacterium belonging to the <i>Erwiniaceae</i> family, is a well-known phytopathogen isolated from many ecological niches and plant hosts. However, this bacterium also provides us with various beneficial characteristics, such as the growth promotion of their host plants and increased crop yield. Some isolated non-pathogenic strains are promising for the microbial production of useful substances. <i>P. ananatis</i> AJ13355 was isolated as an acidophilic bacterium and was used as an excellent host to produce <span style="font-variant: small-caps;">L</span>-glutamic acid under acidic conditions. The genome sequence of <i>P. ananatis</i> AJ13355 was determined, and specific genome-engineering technologies were developed. As a result, <i>P. ananatis</i> was successfully used to construct a bacterial strain that produces cysteine, a sulfur-containing amino acid that has been difficult to produce through fermentation because of complex regulation. Furthermore, by heterologous expression including plant-derived genes, construction of a strain that produces isoprenoids such as isoprene and linalool as secondary metabolites was achieved. <i>P. ananatis</i> is shown to be a useful host for the production of secondary metabolites, as well as amino acids, and is expected to be used as a platform for microbial production of bioactive substances, aromatic substances, and other high-value-added substances of plant origin in the future.https://www.mdpi.com/2076-2607/10/6/1133<i>Pantoea ananatis</i>microbial production<span style="font-variant: small-caps">L</span>-glutamate<span style="font-variant: small-caps">L</span>-cysteineisoprenelinalool |
| spellingShingle | Yoshihiro Usuda Yousuke Nishio Gen Nonaka Yoshihiko Hara Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites Microorganisms <i>Pantoea ananatis</i> microbial production <span style="font-variant: small-caps">L</span>-glutamate <span style="font-variant: small-caps">L</span>-cysteine isoprene linalool |
| title | Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites |
| title_full | Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites |
| title_fullStr | Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites |
| title_full_unstemmed | Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites |
| title_short | Microbial Production Potential of <i>Pantoea ananatis</i>: From Amino Acids to Secondary Metabolites |
| title_sort | microbial production potential of i pantoea ananatis i from amino acids to secondary metabolites |
| topic | <i>Pantoea ananatis</i> microbial production <span style="font-variant: small-caps">L</span>-glutamate <span style="font-variant: small-caps">L</span>-cysteine isoprene linalool |
| url | https://www.mdpi.com/2076-2607/10/6/1133 |
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