Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth
Objective To investigate the outcomes of a novel direct pulp capping agent containing platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA). Methods A total of 32 New Zealand rabbits were randomly divided into 4 groups, namely, the PRF+MTA group (P+M group), PRF group (P group), MTA group...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2023-03-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2023.03.003 |
| _version_ | 1828086769595187200 |
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| author | YANG Xue YAN Zhihao LIU Jin HU Yuping LI Shufang |
| author_facet | YANG Xue YAN Zhihao LIU Jin HU Yuping LI Shufang |
| author_sort | YANG Xue |
| collection | DOAJ |
| description | Objective To investigate the outcomes of a novel direct pulp capping agent containing platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA). Methods A total of 32 New Zealand rabbits were randomly divided into 4 groups, namely, the PRF+MTA group (P+M group), PRF group (P group), MTA group (M group) and blank control group (BC group), with 8 rabbits per group. Dental pulp exposure and direct pulp capping were performed, and complete crown square sealing was performed on 2 mandibular central incisor teeth of each rabbit. Four rabbits from each group were euthanized after each observation period (7 and 28 days). The experimental teeth were subjected to HE staining. Inflammatory cell infiltration, calcified bridge formation and pulp tissue disorganization were observed and graded. Results Inflammatory cell infiltration: on the 7th day, group P+M and group M were lighter than group BC (P<0.05); on the 28th day, group P+M was lighter than group P and group BC (P<0.05); group P+M and group M did not significantly differ (P>0.05). Calcified bridge formation: on the 7th and 28th days, group P+M was lighter than group P, group M and group BC (P<0.05); on the 28th day, group M was higher than group BC (P<0.05). Under microscope, the calcified bridge contained cellular components and was surrounded by odontoblast-like cells, sharing a structure resembled osteodentin; dentin tubule-like structure could not be observed in calcified bridge, and the calcified bridge resembled certain points of osteodentin. Pulp tissue disorganization: on the 7th day, group P+M and group M were lighter than group BC (P<0.05); on the 28th day, group P+M was lighter than group P and group BC (P<0.05). group P+M and group M did not significantly differ (P>0.05). Conclusion The combination of PRF and MTA for direct pulp capping provided light inflammatory cell infiltration, stable pulp status and a strong ability of pulp tissue to form calcified bridge, and the calcified bridge resembled certain points of osteodentin. |
| first_indexed | 2024-04-11T05:00:13Z |
| format | Article |
| id | doaj.art-c9abeba261644f549c7aebaca28aa6de |
| institution | Directory Open Access Journal |
| issn | 2096-1456 |
| language | zho |
| last_indexed | 2024-04-11T05:00:13Z |
| publishDate | 2023-03-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-c9abeba261644f549c7aebaca28aa6de2022-12-26T03:25:34ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562023-03-0131317117810.12016/j.issn.2096⁃1456.2023.03.003Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teethYANG Xue0 YAN Zhihao 1 LIU Jin 2HU Yuping 3LI Shufang 4 Liuzhou People’s Hospital Liuzhou People’s HospitalYoujiang Medical University for NationalitiesYoujiang Medical University for NationalitiesYoujiang Medical University for NationalitiesObjective To investigate the outcomes of a novel direct pulp capping agent containing platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA). Methods A total of 32 New Zealand rabbits were randomly divided into 4 groups, namely, the PRF+MTA group (P+M group), PRF group (P group), MTA group (M group) and blank control group (BC group), with 8 rabbits per group. Dental pulp exposure and direct pulp capping were performed, and complete crown square sealing was performed on 2 mandibular central incisor teeth of each rabbit. Four rabbits from each group were euthanized after each observation period (7 and 28 days). The experimental teeth were subjected to HE staining. Inflammatory cell infiltration, calcified bridge formation and pulp tissue disorganization were observed and graded. Results Inflammatory cell infiltration: on the 7th day, group P+M and group M were lighter than group BC (P<0.05); on the 28th day, group P+M was lighter than group P and group BC (P<0.05); group P+M and group M did not significantly differ (P>0.05). Calcified bridge formation: on the 7th and 28th days, group P+M was lighter than group P, group M and group BC (P<0.05); on the 28th day, group M was higher than group BC (P<0.05). Under microscope, the calcified bridge contained cellular components and was surrounded by odontoblast-like cells, sharing a structure resembled osteodentin; dentin tubule-like structure could not be observed in calcified bridge, and the calcified bridge resembled certain points of osteodentin. Pulp tissue disorganization: on the 7th day, group P+M and group M were lighter than group BC (P<0.05); on the 28th day, group P+M was lighter than group P and group BC (P<0.05). group P+M and group M did not significantly differ (P>0.05). Conclusion The combination of PRF and MTA for direct pulp capping provided light inflammatory cell infiltration, stable pulp status and a strong ability of pulp tissue to form calcified bridge, and the calcified bridge resembled certain points of osteodentin.http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2023.03.003platelet-rich fibrin ，mineral trioxide aggregate ，direct pulp capping ，capping agent ，inflammatory response ，calcified bridge formation ，pulp tissue disorganization ，mandibular central incisor teeth of rabbit ，histological grade， |
| spellingShingle | YANG Xue YAN Zhihao LIU Jin HU Yuping LI Shufang Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth 口腔疾病防治 platelet-rich fibrin ， mineral trioxide aggregate ， direct pulp capping ， capping agent ， inflammatory response ， calcified bridge formation ， pulp tissue disorganization ， mandibular central incisor teeth of rabbit ， histological grade， |
| title | Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth |
| title_full | Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth |
| title_fullStr | Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth |
| title_full_unstemmed | Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth |
| title_short | Histological study of platelet-rich fibrin combined with MTA for direct pulp capping of rabbit teeth |
| title_sort | histological study of platelet rich fibrin combined with mta for direct pulp capping of rabbit teeth |
| topic | platelet-rich fibrin ， mineral trioxide aggregate ， direct pulp capping ， capping agent ， inflammatory response ， calcified bridge formation ， pulp tissue disorganization ， mandibular central incisor teeth of rabbit ， histological grade， |
| url | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2023.03.003 |
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