Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage
As excellent biocontrol agents and plant growth promoters, <i>Trichoderma</i> species are agriculturally important. <i>Trichoderma</i> spp. cultures can be produced using solid-state or submerged cultivation, the latter being much less labor intensive and easier to control an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/1084 |
| _version_ | 1797604196009115648 |
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| author | Alina Rimkus Agne Namina Marija Tereze Dzierkale Oskars Grigs Maris Senkovs Simona Larsson |
| author_facet | Alina Rimkus Agne Namina Marija Tereze Dzierkale Oskars Grigs Maris Senkovs Simona Larsson |
| author_sort | Alina Rimkus |
| collection | DOAJ |
| description | As excellent biocontrol agents and plant growth promoters, <i>Trichoderma</i> species are agriculturally important. <i>Trichoderma</i> spp. cultures can be produced using solid-state or submerged cultivation, the latter being much less labor intensive and easier to control and automate. The aim of the study was to investigate the ability to increase the shelf-life of <i>T. asperellum</i> cells by optimizing cultivation media and upscaling the submerged cultivation process. Four different cultivation media were used with or without the addition of Tween 80 and stored with or without incorporation into peat, and viability, expressed as CFU/g, was assessed during one year of storage in an industrial warehouse. The addition of Tween 80 had a positive effect on the biomass yield. The culture medium played a major role in the ability of the mycelium to produce spores, which in turn influenced the amount of CFU. This effect was less pronounced when the biomass was mixed with peat prior to storage. A procedure that increases the number of CFU in a peat-based product formulation is recommended, namely, incubation of the mixture at 30 °C for 10 days prior to storage at 15 °C over an extended period of time. |
| first_indexed | 2024-03-11T04:42:52Z |
| format | Article |
| id | doaj.art-c9ad3536b3a04c2a983559d2d786ae19 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:42:52Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c9ad3536b3a04c2a983559d2d786ae192023-11-17T20:34:46ZengMDPI AGMicroorganisms2076-26072023-04-01114108410.3390/microorganisms11041084Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during StorageAlina Rimkus0Agne Namina1Marija Tereze Dzierkale2Oskars Grigs3Maris Senkovs4Simona Larsson5Bioefekts Ltd., 30 Livzemes Street, LV-2169 Salaspils, LatviaBioefekts Ltd., 30 Livzemes Street, LV-2169 Salaspils, LatviaBioefekts Ltd., 30 Livzemes Street, LV-2169 Salaspils, LatviaLaboratory of Bioengineering, Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaBioefekts Ltd., 30 Livzemes Street, LV-2169 Salaspils, LatviaBioefekts Ltd., 30 Livzemes Street, LV-2169 Salaspils, LatviaAs excellent biocontrol agents and plant growth promoters, <i>Trichoderma</i> species are agriculturally important. <i>Trichoderma</i> spp. cultures can be produced using solid-state or submerged cultivation, the latter being much less labor intensive and easier to control and automate. The aim of the study was to investigate the ability to increase the shelf-life of <i>T. asperellum</i> cells by optimizing cultivation media and upscaling the submerged cultivation process. Four different cultivation media were used with or without the addition of Tween 80 and stored with or without incorporation into peat, and viability, expressed as CFU/g, was assessed during one year of storage in an industrial warehouse. The addition of Tween 80 had a positive effect on the biomass yield. The culture medium played a major role in the ability of the mycelium to produce spores, which in turn influenced the amount of CFU. This effect was less pronounced when the biomass was mixed with peat prior to storage. A procedure that increases the number of CFU in a peat-based product formulation is recommended, namely, incubation of the mixture at 30 °C for 10 days prior to storage at 15 °C over an extended period of time.https://www.mdpi.com/2076-2607/11/4/1084<i>Trichoderma asperellum</i>viabilitycultivation mediashelf-lifepeat |
| spellingShingle | Alina Rimkus Agne Namina Marija Tereze Dzierkale Oskars Grigs Maris Senkovs Simona Larsson Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage Microorganisms <i>Trichoderma asperellum</i> viability cultivation media shelf-life peat |
| title | Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage |
| title_full | Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage |
| title_fullStr | Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage |
| title_full_unstemmed | Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage |
| title_short | Impact of Growth Conditions on the Viability of <i>Trichoderma asperellum</i> during Storage |
| title_sort | impact of growth conditions on the viability of i trichoderma asperellum i during storage |
| topic | <i>Trichoderma asperellum</i> viability cultivation media shelf-life peat |
| url | https://www.mdpi.com/2076-2607/11/4/1084 |
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