Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation
The aim of this work was the adaptation of a Gas Chromatographic-Flame Ionization Detector (GC-FID) method for detection and quantification of extracellular free amino acids in demineralized water, De Mann Rogosa Sharpe (MRS) medium and corn grits (CG) withdrawn during lactic acid fermentation. In o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2017-01-01
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| Series: | The EuroBiotech Journal |
| Online Access: | https://doi.org/10.24190/ISSN2564-615X/2017/01.05 |
| _version_ | 1829500961207353344 |
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| author | Trontel Antonija Slavica Anita Novak Mario Jelovac Nuša Novak Srđan Šantek Božidar |
| author_facet | Trontel Antonija Slavica Anita Novak Mario Jelovac Nuša Novak Srđan Šantek Božidar |
| author_sort | Trontel Antonija |
| collection | DOAJ |
| description | The aim of this work was the adaptation of a Gas Chromatographic-Flame Ionization Detector (GC-FID) method for detection and quantification of extracellular free amino acids in demineralized water, De Mann Rogosa Sharpe (MRS) medium and corn grits (CG) withdrawn during lactic acid fermentation. In order to analyze free amino acids by the GC-FID method it was necessary to convert free amino acids to volatile compounds. This was accomplished by derivatization of free amino acids with ethylchlor formate in aqueous medium followed by extraction of volatile free amino acid esters with chloroform. It was proven that the combination of derivatization and extraction procedure with developed GC-FID method gave accurate, reproducible and sensitive analytical results. Quantification of 15 (Ala, Gly, Val, Leu, Ile, Phe, Tyr, Trp, Asn, Met, Pro, Lys, His, Asp and Glu) out of 20 ethoxycarbonyl-ethyl esters of free amino acids in demineralized water and MRS medium was achieved by established methods. In corn grits medium all of the above mentioned 15 amino acids, except His, were quantified with this GC-FID method. The established method was efficiently verified in monitoring of extracellular free amino acid concentration during lactic acid production with Lactobacillus rhamnosus DSM 20021T in MRS medium and Lactobacillus amylovorus DSM 20531T in corn grits medium. |
| first_indexed | 2024-12-16T09:08:30Z |
| format | Article |
| id | doaj.art-c9ae24c0261c4a4589f7018ec731b778 |
| institution | Directory Open Access Journal |
| issn | 2564-615X |
| language | English |
| last_indexed | 2024-12-16T09:08:30Z |
| publishDate | 2017-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj.art-c9ae24c0261c4a4589f7018ec731b7782022-12-21T22:37:02ZengSciendoThe EuroBiotech Journal2564-615X2017-01-0111273510.24190/ISSN2564-615X/2017/01.05Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentationTrontel Antonija0Slavica Anita1Novak Mario2Jelovac Nuša3Novak Srđan4Šantek Božidar5Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaLaboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaLaboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaLaboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaLaboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaLaboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaThe aim of this work was the adaptation of a Gas Chromatographic-Flame Ionization Detector (GC-FID) method for detection and quantification of extracellular free amino acids in demineralized water, De Mann Rogosa Sharpe (MRS) medium and corn grits (CG) withdrawn during lactic acid fermentation. In order to analyze free amino acids by the GC-FID method it was necessary to convert free amino acids to volatile compounds. This was accomplished by derivatization of free amino acids with ethylchlor formate in aqueous medium followed by extraction of volatile free amino acid esters with chloroform. It was proven that the combination of derivatization and extraction procedure with developed GC-FID method gave accurate, reproducible and sensitive analytical results. Quantification of 15 (Ala, Gly, Val, Leu, Ile, Phe, Tyr, Trp, Asn, Met, Pro, Lys, His, Asp and Glu) out of 20 ethoxycarbonyl-ethyl esters of free amino acids in demineralized water and MRS medium was achieved by established methods. In corn grits medium all of the above mentioned 15 amino acids, except His, were quantified with this GC-FID method. The established method was efficiently verified in monitoring of extracellular free amino acid concentration during lactic acid production with Lactobacillus rhamnosus DSM 20021T in MRS medium and Lactobacillus amylovorus DSM 20531T in corn grits medium.https://doi.org/10.24190/ISSN2564-615X/2017/01.05 |
| spellingShingle | Trontel Antonija Slavica Anita Novak Mario Jelovac Nuša Novak Srđan Šantek Božidar Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation The EuroBiotech Journal |
| title | Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation |
| title_full | Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation |
| title_fullStr | Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation |
| title_full_unstemmed | Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation |
| title_short | Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation |
| title_sort | applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentation |
| url | https://doi.org/10.24190/ISSN2564-615X/2017/01.05 |
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