Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria
The absence of novel, safe, and effective bactericide is an urgent concern worldwide and remains a challenge in scientific communities. The unique proprieties of silver nanoparticles (SNPs) synthesized from plant extracts make them a suitable candidate to overcome these limitations. Herein, we synth...
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MDPI AG
2022-07-01
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| author | Michele Stella Majoumouo Marius Belmondo Tincho Youmbi Diane Yimta Tayo Alex Adekiya Raphael Taiwo Aruleba Nimibofa Ayawei Fabrice Fekam Boyom Thureyah Morris |
| author_facet | Michele Stella Majoumouo Marius Belmondo Tincho Youmbi Diane Yimta Tayo Alex Adekiya Raphael Taiwo Aruleba Nimibofa Ayawei Fabrice Fekam Boyom Thureyah Morris |
| author_sort | Michele Stella Majoumouo |
| collection | DOAJ |
| description | The absence of novel, safe, and effective bactericide is an urgent concern worldwide and remains a challenge in scientific communities. The unique proprieties of silver nanoparticles (SNPs) synthesized from plant extracts make them a suitable candidate to overcome these limitations. Herein, we synthesized SNPs from <i>Bersama engleriana</i> fruit (BEfr) extracts and determined their potential antibacterial activity and mode of action. SNPs were synthesized from BEfr methanolic fruit extracts at 25 and 70 °C, and the antibacterial effectiveness of SNPs against bacterial strains was investigated. The surface plasmon resonance peaked at 430.18 and 434.08 nm, respectively, for SNPs synthesized at 25 and 70 °C, confirming SNPs synthesis. BEfr-SNPs had minimum inhibitory concentrations (MIC) range of 0.234 to >50 µg/mL, which was 30-fold greater than extract alone (MIC of 500 µg/mL). BEfr-SNPs-25 °C was potent against six bacterial strains (<i>S. aureus</i>, <i>S. enterica</i>, <i>MRS. aureus</i>, <i>K. pneumonia</i>, and <i>S. pyogenes</i>), with MIC range of 0.339 to 6.25 µg/mL. The mode of action of BEfr-SNPs-25 °C was achieved by an <i>MRSA</i> bacteria strain outer membrane and DNA nucleotide linkage. These results suggest that our synthesized SNPs, especially BEfr-SNPs-25 °C, demonstrated an enhanced antibacterial effect and could be potential candidates for bacterial infection treatment. |
| first_indexed | 2024-03-09T12:03:06Z |
| format | Article |
| id | doaj.art-c9af9e89c6b14eae9f3063d2711e4896 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T12:03:06Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-c9af9e89c6b14eae9f3063d2711e48962023-11-30T23:01:46ZengMDPI AGCrystals2073-43522022-07-01127101010.3390/cryst12071010Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant BacteriaMichele Stella Majoumouo0Marius Belmondo Tincho1Youmbi Diane Yimta2Tayo Alex Adekiya3Raphael Taiwo Aruleba4Nimibofa Ayawei5Fabrice Fekam Boyom6Thureyah Morris7Antimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, University of Yaoundé 1, Yaoundé PO. Box 812, CameroonDepartment of Biotechnology, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535, South AfricaAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, University of Yaoundé 1, Yaoundé PO. Box 812, CameroonDepartment of Pharmaceutical Sciences, Howard University, Washington, DC 20059, USADepartment of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Cape Town 7701, South AfricaDepartment of Chemistry, Bayelsa Medical University, Yenagoa PMB 178, NigeriaAntimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, University of Yaoundé 1, Yaoundé PO. Box 812, CameroonDepartment of Medical Bioscience, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535, South AfricaThe absence of novel, safe, and effective bactericide is an urgent concern worldwide and remains a challenge in scientific communities. The unique proprieties of silver nanoparticles (SNPs) synthesized from plant extracts make them a suitable candidate to overcome these limitations. Herein, we synthesized SNPs from <i>Bersama engleriana</i> fruit (BEfr) extracts and determined their potential antibacterial activity and mode of action. SNPs were synthesized from BEfr methanolic fruit extracts at 25 and 70 °C, and the antibacterial effectiveness of SNPs against bacterial strains was investigated. The surface plasmon resonance peaked at 430.18 and 434.08 nm, respectively, for SNPs synthesized at 25 and 70 °C, confirming SNPs synthesis. BEfr-SNPs had minimum inhibitory concentrations (MIC) range of 0.234 to >50 µg/mL, which was 30-fold greater than extract alone (MIC of 500 µg/mL). BEfr-SNPs-25 °C was potent against six bacterial strains (<i>S. aureus</i>, <i>S. enterica</i>, <i>MRS. aureus</i>, <i>K. pneumonia</i>, and <i>S. pyogenes</i>), with MIC range of 0.339 to 6.25 µg/mL. The mode of action of BEfr-SNPs-25 °C was achieved by an <i>MRSA</i> bacteria strain outer membrane and DNA nucleotide linkage. These results suggest that our synthesized SNPs, especially BEfr-SNPs-25 °C, demonstrated an enhanced antibacterial effect and could be potential candidates for bacterial infection treatment.https://www.mdpi.com/2073-4352/12/7/1010green nanotechnology<i>Bersama engleriana</i>antibacterial activitymode of action |
| spellingShingle | Michele Stella Majoumouo Marius Belmondo Tincho Youmbi Diane Yimta Tayo Alex Adekiya Raphael Taiwo Aruleba Nimibofa Ayawei Fabrice Fekam Boyom Thureyah Morris Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria Crystals green nanotechnology <i>Bersama engleriana</i> antibacterial activity mode of action |
| title | Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria |
| title_full | Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria |
| title_fullStr | Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria |
| title_full_unstemmed | Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria |
| title_short | Biosynthesis of Silver Nanoparticles Using <i>Bersama engleriana</i> Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria |
| title_sort | biosynthesis of silver nanoparticles using i bersama engleriana i fruits extracts and their potential inhibitory effect on resistant bacteria |
| topic | green nanotechnology <i>Bersama engleriana</i> antibacterial activity mode of action |
| url | https://www.mdpi.com/2073-4352/12/7/1010 |
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