Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield
Abstract Background The application of the sterile insect technique (SIT) requires mass-production of sterile males of good biological quality. The size of the project area will in most cases determine whether it is more cost effective to produce the sterile flies locally (and invest in a mass-reari...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2015-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-0869-3 |
| _version_ | 1827933797526536192 |
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| author | Soumaïla Pagabeleguem Momar Talla Seck Baba Sall Marc JB Vreysen Geoffrey Gimonneau Assane Gueye Fall Mireille Bassene Issa Sidibé Jean-Baptiste Rayaissé Adrien MG Belem Jérémy Bouyer |
| author_facet | Soumaïla Pagabeleguem Momar Talla Seck Baba Sall Marc JB Vreysen Geoffrey Gimonneau Assane Gueye Fall Mireille Bassene Issa Sidibé Jean-Baptiste Rayaissé Adrien MG Belem Jérémy Bouyer |
| author_sort | Soumaïla Pagabeleguem |
| collection | DOAJ |
| description | Abstract Background The application of the sterile insect technique (SIT) requires mass-production of sterile males of good biological quality. The size of the project area will in most cases determine whether it is more cost effective to produce the sterile flies locally (and invest in a mass-rearing facility) or import the sterile flies from a mass-rearing facility that is located in another country. This study aimed at assessing the effect of long distance transport of sterile male Glossina palpalis gambiensis pupae on adult male fly yield. Methods The male pupae were produced at the Centre International de Recherche-Développement sur l’Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso, Burkina Faso, and shipped with a commercial courier service in insulated transport boxes at a temperature of ±10°C to Senegal (±36 h of transport). Upon arrival in the insectary in Dakar, the pupae were transferred to an emergence room and the flies monitored for 3–6 days. Results The results showed that the used system of isothermal boxes that contained phase change material packs (S8) managed to keep the temperature at around 10°C which prevented male fly emergence during transport. The emergence rate was significantly higher for pupae from batch 2 (chilled at 4°C for one day in the source insectary before transport) than those from batch 1 (chilled at 4°C for two days in the source insectary before transport) i.e. an average (±sd) of 76.1 ± 13.2% and 72.2 ± 14.3%, respectively with a small proportion emerging during transport (0.7 ± 1.7% and 0.9 ± 2.9%, respectively). Among the emerged flies, the percentage with deformed (not fully expanded) wings was significantly higher for flies from batch 1 (12.0 ± 6.3%) than from batch 2 (10.7 ± 7.5%). The amount of sterile males available for release as a proportion of the total pupae shipped was 65.8 ± 13.3% and 61.7 ± 14.7% for batch 1 and 2 pupae, respectively. Conclusions The results also showed that the temperature inside the parcel must be controlled around 10°C with a maximal deviation of 3°C to maximize the male yield. |
| first_indexed | 2024-03-13T07:29:49Z |
| format | Article |
| id | doaj.art-c9afdd28f85a45e28c510b46792bbb08 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:49Z |
| publishDate | 2015-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c9afdd28f85a45e28c510b46792bbb082023-06-04T11:10:44ZengBMCParasites & Vectors1756-33052015-05-01811910.1186/s13071-015-0869-3Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yieldSoumaïla Pagabeleguem0Momar Talla Seck1Baba Sall2Marc JB Vreysen3Geoffrey Gimonneau4Assane Gueye Fall5Mireille Bassene6Issa Sidibé7Jean-Baptiste Rayaissé8Adrien MG Belem9Jérémy Bouyer10Pan-African Tsetse and Trypanosomosis Eradication CampaignInstitut Sénégalais de Recherches Agricoles, Laboratoire National d’Elevage et de Recherches Vétérinaires, Service de ParasitologieDirection des Services VétérinairesInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and AgricultureCentre de Coopération Internationale en Recherche Agronomique pour le Développement, Unité Mixte de Recherche Contrôle des Maladies Animales Exotiques et Emergentes, Campus International de BaillarguetInstitut Sénégalais de Recherches Agricoles, Laboratoire National d’Elevage et de Recherches Vétérinaires, Service de ParasitologieInstitut Sénégalais de Recherches Agricoles, Laboratoire National d’Elevage et de Recherches Vétérinaires, Service de ParasitologiePan-African Tsetse and Trypanosomosis Eradication CampaignCentre International de Recherche-développement sur l’Élevage en Zone SubhumideUniversité Polytechnique de Bobo-DioulassoCentre de Coopération Internationale en Recherche Agronomique pour le Développement, Unité Mixte de Recherche Contrôle des Maladies Animales Exotiques et Emergentes, Campus International de BaillarguetAbstract Background The application of the sterile insect technique (SIT) requires mass-production of sterile males of good biological quality. The size of the project area will in most cases determine whether it is more cost effective to produce the sterile flies locally (and invest in a mass-rearing facility) or import the sterile flies from a mass-rearing facility that is located in another country. This study aimed at assessing the effect of long distance transport of sterile male Glossina palpalis gambiensis pupae on adult male fly yield. Methods The male pupae were produced at the Centre International de Recherche-Développement sur l’Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso, Burkina Faso, and shipped with a commercial courier service in insulated transport boxes at a temperature of ±10°C to Senegal (±36 h of transport). Upon arrival in the insectary in Dakar, the pupae were transferred to an emergence room and the flies monitored for 3–6 days. Results The results showed that the used system of isothermal boxes that contained phase change material packs (S8) managed to keep the temperature at around 10°C which prevented male fly emergence during transport. The emergence rate was significantly higher for pupae from batch 2 (chilled at 4°C for one day in the source insectary before transport) than those from batch 1 (chilled at 4°C for two days in the source insectary before transport) i.e. an average (±sd) of 76.1 ± 13.2% and 72.2 ± 14.3%, respectively with a small proportion emerging during transport (0.7 ± 1.7% and 0.9 ± 2.9%, respectively). Among the emerged flies, the percentage with deformed (not fully expanded) wings was significantly higher for flies from batch 1 (12.0 ± 6.3%) than from batch 2 (10.7 ± 7.5%). The amount of sterile males available for release as a proportion of the total pupae shipped was 65.8 ± 13.3% and 61.7 ± 14.7% for batch 1 and 2 pupae, respectively. Conclusions The results also showed that the temperature inside the parcel must be controlled around 10°C with a maximal deviation of 3°C to maximize the male yield.https://doi.org/10.1186/s13071-015-0869-3Area-wide integrated pest managementSterile insect techniquePupae developmentLow temperaturesPupae transportMass-rearing |
| spellingShingle | Soumaïla Pagabeleguem Momar Talla Seck Baba Sall Marc JB Vreysen Geoffrey Gimonneau Assane Gueye Fall Mireille Bassene Issa Sidibé Jean-Baptiste Rayaissé Adrien MG Belem Jérémy Bouyer Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield Parasites & Vectors Area-wide integrated pest management Sterile insect technique Pupae development Low temperatures Pupae transport Mass-rearing |
| title | Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield |
| title_full | Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield |
| title_fullStr | Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield |
| title_full_unstemmed | Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield |
| title_short | Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield |
| title_sort | long distance transport of irradiated male glossina palpalis gambiensis pupae and its impact on sterile male yield |
| topic | Area-wide integrated pest management Sterile insect technique Pupae development Low temperatures Pupae transport Mass-rearing |
| url | https://doi.org/10.1186/s13071-015-0869-3 |
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