Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster
Genetic variants are considered as one of the main determinants of the concentration of serum lipids and coronary artery disease (CAD). Polymorphisms in the Apolipoprotein (Apo) AI-CIII-AIV gene cluster has been known to affect the concentrations of various lipid sub-fractions and the risk of CAD. T...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-12-01
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| Series: | Cogent Medicine |
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| Online Access: | http://dx.doi.org/10.1080/2331205X.2016.1266789 |
| _version_ | 1818025036616826880 |
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| author | Himanshu Rai Nakul Sinha James Finn Suraksha Agrawal Sarabjit Mastana |
| author_facet | Himanshu Rai Nakul Sinha James Finn Suraksha Agrawal Sarabjit Mastana |
| author_sort | Himanshu Rai |
| collection | DOAJ |
| description | Genetic variants are considered as one of the main determinants of the concentration of serum lipids and coronary artery disease (CAD). Polymorphisms in the Apolipoprotein (Apo) AI-CIII-AIV gene cluster has been known to affect the concentrations of various lipid sub-fractions and the risk of CAD. The present study assessed associations between polymorphisms of the Apo AI-CIII-AIV gene cluster, [ApoA-I,-75G > A, (rs1799837); ApoC-III 3238C > G, (SstI), (rs5128) and ApoA-IV, Thr347Ser(347A > T), (rs675)] with serum lipids and their contributions to CAD in North Indian population. We recruited age, sex matched, 200 CAD patients and 200 healthy controls and tested them for fasting levels of serum lipids. We genotyped selected polymorphisms using polymerase chain reaction-restriction fragment length polymorphism. There were no statistically significant association of selected polymorphisms (or their combinations) with CAD even after employing additive, dominant and recessive models. However there was significant association of selected polymorphisms with various lipid traits amongst the control cohort (p < 0.05). Mean levels of high density lipoprotein cholesterol and triglycerides were found to be significantly higher among controls carrying at least one mutant allele at ApoA1-75G > A (p = 0.019) and ApoCIII SstI (p < 0.001) polymorphism respectively. Our study observed that the selected polymorphisms in the ApoAI-CIII-AIV gene cluster although significantly affect various lipid traits but this affect does not seem to translate into association with CAD, at least among North Indian population. |
| first_indexed | 2024-12-10T04:09:43Z |
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| id | doaj.art-c9b043f2b418471b8df4c5fb1ecc5ca0 |
| institution | Directory Open Access Journal |
| issn | 2331-205X |
| language | English |
| last_indexed | 2024-12-10T04:09:43Z |
| publishDate | 2016-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Medicine |
| spelling | doaj.art-c9b043f2b418471b8df4c5fb1ecc5ca02022-12-22T02:02:46ZengTaylor & Francis GroupCogent Medicine2331-205X2016-12-013110.1080/2331205X.2016.12667891266789Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene clusterHimanshu Rai0Nakul Sinha1James Finn2Suraksha Agrawal3Sarabjit Mastana4Sanjay Gandhi Post Graduate Institute of Medical SciencesSanjay Gandhi Post Graduate Institute of Medical SciencesLoughborough UniversitySanjay Gandhi Post Graduate Institute of Medical SciencesLoughborough UniversityGenetic variants are considered as one of the main determinants of the concentration of serum lipids and coronary artery disease (CAD). Polymorphisms in the Apolipoprotein (Apo) AI-CIII-AIV gene cluster has been known to affect the concentrations of various lipid sub-fractions and the risk of CAD. The present study assessed associations between polymorphisms of the Apo AI-CIII-AIV gene cluster, [ApoA-I,-75G > A, (rs1799837); ApoC-III 3238C > G, (SstI), (rs5128) and ApoA-IV, Thr347Ser(347A > T), (rs675)] with serum lipids and their contributions to CAD in North Indian population. We recruited age, sex matched, 200 CAD patients and 200 healthy controls and tested them for fasting levels of serum lipids. We genotyped selected polymorphisms using polymerase chain reaction-restriction fragment length polymorphism. There were no statistically significant association of selected polymorphisms (or their combinations) with CAD even after employing additive, dominant and recessive models. However there was significant association of selected polymorphisms with various lipid traits amongst the control cohort (p < 0.05). Mean levels of high density lipoprotein cholesterol and triglycerides were found to be significantly higher among controls carrying at least one mutant allele at ApoA1-75G > A (p = 0.019) and ApoCIII SstI (p < 0.001) polymorphism respectively. Our study observed that the selected polymorphisms in the ApoAI-CIII-AIV gene cluster although significantly affect various lipid traits but this affect does not seem to translate into association with CAD, at least among North Indian population.http://dx.doi.org/10.1080/2331205X.2016.1266789apolipoprotein (apo) ai-ciii-aiv gene clustersingle nucleotide polymorphisms (snps)serum lipidscoronary artery disease (cad)linkage disequilibrium, haplotypes |
| spellingShingle | Himanshu Rai Nakul Sinha James Finn Suraksha Agrawal Sarabjit Mastana Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster Cogent Medicine apolipoprotein (apo) ai-ciii-aiv gene cluster single nucleotide polymorphisms (snps) serum lipids coronary artery disease (cad) linkage disequilibrium, haplotypes |
| title | Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster |
| title_full | Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster |
| title_fullStr | Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster |
| title_full_unstemmed | Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster |
| title_short | Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster |
| title_sort | association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein ai ciii aiv gene cluster |
| topic | apolipoprotein (apo) ai-ciii-aiv gene cluster single nucleotide polymorphisms (snps) serum lipids coronary artery disease (cad) linkage disequilibrium, haplotypes |
| url | http://dx.doi.org/10.1080/2331205X.2016.1266789 |
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