Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor
This study develops a highly sensitive and low-cost carboxyl-graphene-oxide-based planar optical waveguide localized surface plasmon resonance biosensor (GO-OW LSPR biosensor), a system based on measuring light intensity changes. The structure of the sensing chip comprises an optical waveguide (OW)-...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/12/13/2146 |
| _version_ | 1797408528641556480 |
|---|---|
| author | Chien-Hsing Chen Chang-Yue Chiang |
| author_facet | Chien-Hsing Chen Chang-Yue Chiang |
| author_sort | Chien-Hsing Chen |
| collection | DOAJ |
| description | This study develops a highly sensitive and low-cost carboxyl-graphene-oxide-based planar optical waveguide localized surface plasmon resonance biosensor (GO-OW LSPR biosensor), a system based on measuring light intensity changes. The structure of the sensing chip comprises an optical waveguide (OW)-slide glass and microfluidic-poly (methyl methacrylate) (PMMA) substrate, and the OW-slide glass surface-modified gold nanoparticle (AuNP) combined with graphene oxide (GO). As the GO has an abundant carboxyl group (–COOH), the number of capture molecules can be increased. The refractive index sensing system uses silver-coated reflective film to compare the refractive index sensitivity of the GO-OW LSPR biosensor to increase the refractive index sensitivity. The result shows that the signal variation of the system with the silver-coated reflective film is 1.57 times that of the system without the silver-coated reflective film. The refractive index sensitivity is 5.48 RIU<sup>−1</sup> and the sensor resolution is 2.52 ± 0.23 × 10<sup>−6</sup> RIU. The biochemical sensing experiment performs immunoglobulin G (IgG) and streptavidin detection. The limits of detection of the sensor for IgG and streptavidin are calculated to be 23.41 ± 1.54 pg/mL and 5.18 ± 0.50 pg/mL, respectively. The coefficient of variation (CV) of the repeatability experiment (sample numbers = 3) is smaller than 10.6%. In addition, the affinity constants of the sensor for anti-IgG/IgG and biotin/streptavidin are estimated to be 1.06 × 10<sup>7</sup> M<sup>−1</sup> and 7.30 × 10<sup>9</sup> M<sup>−1</sup>, respectively. The result shows that the GO-OW LSPR biosensor has good repeatability and very low detection sensitivity. It can be used for detecting low concentrations or small biomolecules in the future. |
| first_indexed | 2024-03-09T04:00:49Z |
| format | Article |
| id | doaj.art-c9b2eb9a863e4faaa6b0802246f71674 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T04:00:49Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-c9b2eb9a863e4faaa6b0802246f716742023-12-03T14:14:34ZengMDPI AGNanomaterials2079-49912022-06-011213214610.3390/nano12132146Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance BiosensorChien-Hsing Chen0Chang-Yue Chiang1Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, Pingtung 91201, TaiwanGraduate School of Engineering Science and Technology and Bachelor Program in Interdisciplinary Studies, National Yunlin University of Science and Technology, Yunlin 64002, TaiwanThis study develops a highly sensitive and low-cost carboxyl-graphene-oxide-based planar optical waveguide localized surface plasmon resonance biosensor (GO-OW LSPR biosensor), a system based on measuring light intensity changes. The structure of the sensing chip comprises an optical waveguide (OW)-slide glass and microfluidic-poly (methyl methacrylate) (PMMA) substrate, and the OW-slide glass surface-modified gold nanoparticle (AuNP) combined with graphene oxide (GO). As the GO has an abundant carboxyl group (–COOH), the number of capture molecules can be increased. The refractive index sensing system uses silver-coated reflective film to compare the refractive index sensitivity of the GO-OW LSPR biosensor to increase the refractive index sensitivity. The result shows that the signal variation of the system with the silver-coated reflective film is 1.57 times that of the system without the silver-coated reflective film. The refractive index sensitivity is 5.48 RIU<sup>−1</sup> and the sensor resolution is 2.52 ± 0.23 × 10<sup>−6</sup> RIU. The biochemical sensing experiment performs immunoglobulin G (IgG) and streptavidin detection. The limits of detection of the sensor for IgG and streptavidin are calculated to be 23.41 ± 1.54 pg/mL and 5.18 ± 0.50 pg/mL, respectively. The coefficient of variation (CV) of the repeatability experiment (sample numbers = 3) is smaller than 10.6%. In addition, the affinity constants of the sensor for anti-IgG/IgG and biotin/streptavidin are estimated to be 1.06 × 10<sup>7</sup> M<sup>−1</sup> and 7.30 × 10<sup>9</sup> M<sup>−1</sup>, respectively. The result shows that the GO-OW LSPR biosensor has good repeatability and very low detection sensitivity. It can be used for detecting low concentrations or small biomolecules in the future.https://www.mdpi.com/2079-4991/12/13/2146graphene oxidewaveguidegold nanoparticlelocalized surface plasmon resonanceanti-IgG-IgGbiotin-streptavidin |
| spellingShingle | Chien-Hsing Chen Chang-Yue Chiang Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor Nanomaterials graphene oxide waveguide gold nanoparticle localized surface plasmon resonance anti-IgG-IgG biotin-streptavidin |
| title | Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor |
| title_full | Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor |
| title_fullStr | Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor |
| title_full_unstemmed | Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor |
| title_short | Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor |
| title_sort | determination of the highly sensitive carboxyl graphene oxide based planar optical waveguide localized surface plasmon resonance biosensor |
| topic | graphene oxide waveguide gold nanoparticle localized surface plasmon resonance anti-IgG-IgG biotin-streptavidin |
| url | https://www.mdpi.com/2079-4991/12/13/2146 |
| work_keys_str_mv | AT chienhsingchen determinationofthehighlysensitivecarboxylgrapheneoxidebasedplanaropticalwaveguidelocalizedsurfaceplasmonresonancebiosensor AT changyuechiang determinationofthehighlysensitivecarboxylgrapheneoxidebasedplanaropticalwaveguidelocalizedsurfaceplasmonresonancebiosensor |