Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil
ABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls fro...
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| Format: | Article |
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Universidade Federal de Minas Gerais
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100197&lng=en&tlng=en |
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| author | A. Henzel P.F. Salla A.K. Mascitti M. Demoliner M.C. Solyman V.R. Lunge F.R. Spilki |
| author_facet | A. Henzel P.F. Salla A.K. Mascitti M. Demoliner M.C. Solyman V.R. Lunge F.R. Spilki |
| author_sort | A. Henzel |
| collection | DOAJ |
| description | ABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls from beef cattle farms located in RS. A total of 372 serum and semen sample from bulls were collected in eighteen farms. Serum samples were submitted to virus neutralization (VN) assay, while semen samples were used to detect BoHV-1 and BoHV-5 DNA by PCR. VN results showed that BoHV-1/5 antibodies were detected in bulls of 66.7% (12/18) of the farms, 295 (79.5%) BoHV positive bulls, 287 for BoHV-1 and 234 for BoHV-; at 43 vaccinated bulls 72.1% (31/43) showing serology negative. BoHV-1/5 DNA was detected in the semen of three bulls; one of the them presenting BoHV-1, one out three presenting BoHV-5 and one BoHV-1/5.co-infection All BoHV DNA positive samples came from animals presenting posthitis and other genital lesions at sampling. Results showed a high seroprevalence of BoHV-1/5 antibodies in bulls as well as strong evidence that these viruses are actively circulating in the cattle farms. A remarkable finding is that in the presence of clinically evident lesions in the genital tract, both BoHV-1 and 5 may found in semen. |
| first_indexed | 2024-12-23T20:40:32Z |
| format | Article |
| id | doaj.art-c9b3be91a1754c64a3aab31b37ed092b |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-23T20:40:32Z |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-c9b3be91a1754c64a3aab31b37ed092b2022-12-21T17:31:56ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-416271119720310.1590/1678-4162-10310S0102-09352019000100197Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in BrazilA. HenzelP.F. SallaA.K. MascittiM. DemolinerM.C. SolymanV.R. LungeF.R. SpilkiABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls from beef cattle farms located in RS. A total of 372 serum and semen sample from bulls were collected in eighteen farms. Serum samples were submitted to virus neutralization (VN) assay, while semen samples were used to detect BoHV-1 and BoHV-5 DNA by PCR. VN results showed that BoHV-1/5 antibodies were detected in bulls of 66.7% (12/18) of the farms, 295 (79.5%) BoHV positive bulls, 287 for BoHV-1 and 234 for BoHV-; at 43 vaccinated bulls 72.1% (31/43) showing serology negative. BoHV-1/5 DNA was detected in the semen of three bulls; one of the them presenting BoHV-1, one out three presenting BoHV-5 and one BoHV-1/5.co-infection All BoHV DNA positive samples came from animals presenting posthitis and other genital lesions at sampling. Results showed a high seroprevalence of BoHV-1/5 antibodies in bulls as well as strong evidence that these viruses are actively circulating in the cattle farms. A remarkable finding is that in the presence of clinically evident lesions in the genital tract, both BoHV-1 and 5 may found in semen.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100197&lng=en&tlng=enBoHV-1 e 5coinfecçãoDNAsêmen |
| spellingShingle | A. Henzel P.F. Salla A.K. Mascitti M. Demoliner M.C. Solyman V.R. Lunge F.R. Spilki Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil Arquivo Brasileiro de Medicina Veterinária e Zootecnia BoHV-1 e 5 coinfecção DNA sêmen |
| title | Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil |
| title_full | Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil |
| title_fullStr | Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil |
| title_full_unstemmed | Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil |
| title_short | Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil |
| title_sort | bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in brazil |
| topic | BoHV-1 e 5 coinfecção DNA sêmen |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100197&lng=en&tlng=en |
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