Detection of Extended Spectrum and Chromosomal Beta-lactamases in Nosocomial Gram Negative Bacteria
The gram negative strains which produce plasmid mediated extended spectrum beta-lactamases (ESBL) or inducible beta-lactamases are common at hospitals. To demonstrate the production of beta-lactamases in hospital strains is important in order to choose the proper antibiotics which are going to be us...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bilimsel Tip Yayinevi
1999-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1999-4-2-102-106.pdf |
| Summary: | The gram negative strains which produce plasmid mediated extended spectrum beta-lactamases (ESBL) or inducible beta-lactamases are common at hospitals. To demonstrate the production of beta-lactamases in hospital strains is important in order to choose the proper antibiotics which are going to be used for the treatment of these strains. This study is done for determining the rates of ESBL and inducible beta-lactamases and to establish whether there is any need for the routine examination. One hundred and thirty-eigth gram negative bacteria which were defined as nosocomial infection agents by the hospital infection control committee between October 1996-June 1997 were used in the study. Double disk synergy test was used to determine ESBL and direct induction test was used to designate inducible beta-lactamase. ESBL is determined among 34 of 55 Klebsiella pneumoniae (61.8%), 1 of 4 K. oxytoca, 2 of 25 Escherichia coli, 1 of 26 Enterobacter spp. and 4 of 5 Salmonella spp. strains. Chromosomal beta-lactamase resistance is determined in 16 of 17 Pseudomonas spp. (94.1%), 20 of 26 Enterobacter spp. (76.9%), 2 of 55 K. pneumoniae and 2 of 3 Citrobacter spp. strains. Nine of chromosomal beta-lactamases were inducible and 31 were constitutive. All inducible beta-lactamases were determined at Pseudomonas species. |
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| ISSN: | 1300-932X 1300-932X |