Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity
Objective: To determine the effect of Salvianolic acid B (Sal B) on glucose and lipid metabolism in mice with high-fat diet (HFD)-induced obesity, and to investigate the underlying mechanisms by measuring the expression levels of key adipogenic transcription factors. Methods: Six-week-old C57BL/6J m...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754817300923 |
| _version_ | 1797725228800933888 |
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| author | Dandan Zhao Jiacheng Zuo Na Yu Xin Fang Fangfang Mo Rui Wu Tian Tian Rufeng Ma Yushan Gao Dongwei Zhang Guangjian Jiang Sihua Gao |
| author_facet | Dandan Zhao Jiacheng Zuo Na Yu Xin Fang Fangfang Mo Rui Wu Tian Tian Rufeng Ma Yushan Gao Dongwei Zhang Guangjian Jiang Sihua Gao |
| author_sort | Dandan Zhao |
| collection | DOAJ |
| description | Objective: To determine the effect of Salvianolic acid B (Sal B) on glucose and lipid metabolism in mice with high-fat diet (HFD)-induced obesity, and to investigate the underlying mechanisms by measuring the expression levels of key adipogenic transcription factors.
Methods: Six-week-old C57BL/6J male mice were fed for 12 weeks with a HFD to induce obesity or a standard diet to serve as normal controls. A mean body weight increase of more than 20% after these 12 weeks was used as the criteria for obesity. HFD-fed obese mice then received a supplement of Sal B (100 mg/kg body weight/day), metformin (75 mg/kg body weight/day) or water (an equivalent volume; served as model controls) by oral gavage for an additional 8 weeks, and the normal controls received water (an equivalent volume) by oral gavage for the same period.
Results: Sal B significantly reduced body weight gain (P < .05) without influencing food intake in HFD-fed obese mice relative to model controls. Sal B also reduced the body fat mass of the obese mice relative to model controls in a time-dependent manner (P < .05). Sal B significantly decreased the serum concentrations of low-density lipoprotein cholesterol, total cholesterol, triglyceride and free fatty acids by 25.5%, 20.2%, 20.6% and 13.4%, respectively, and increased the concentration of high-density lipoprotein cholesterol by 50.1% relative to model controls. In addition, Sal B significantly lowered fasting glucose concentrations and improved insulin sensitivity relative to model controls (P < .05). Sal B acted by ameliorating the histopathological changes in both brown and white adipose tissues of obese mice. Moreover, in brown adipose tissue, Sal B up-regulated the mRNA and protein expression of PPARγ and c/EBPα, and the protein expression of PPARα and SREBP-1 (P < .05). In white adipose tissue, Sal B down-regulated the mRNA expression of PPARγ and c/EBPα, and decreased the protein expression of PPARγ and SREBP-1(P < .05).
Conclusions: The results suggest that Sal B can reduce body weight gain and regulate glucose and lipid metabolism in mice with diet-induced obesity by regulating adipogenic transcription factors in their adipose tissues. |
| first_indexed | 2024-03-12T10:28:09Z |
| format | Article |
| id | doaj.art-c9b975fc10b84661821454aa22458fb4 |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T10:28:09Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-c9b975fc10b84661821454aa22458fb42023-09-02T09:32:27ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482017-07-014328028910.1016/j.jtcms.2017.07.003Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesityDandan Zhao0Jiacheng Zuo1Na Yu2Xin Fang3Fangfang Mo4Rui Wu5Tian Tian6Rufeng Ma7Yushan Gao8Dongwei Zhang9Guangjian Jiang10Sihua Gao11Beijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaDepartment of Endocrinology, Beijing University of Chinese Medicine, Third Affiliated Hospital, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaDepartment of Endocrinology, South Area of Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 102618, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaObjective: To determine the effect of Salvianolic acid B (Sal B) on glucose and lipid metabolism in mice with high-fat diet (HFD)-induced obesity, and to investigate the underlying mechanisms by measuring the expression levels of key adipogenic transcription factors. Methods: Six-week-old C57BL/6J male mice were fed for 12 weeks with a HFD to induce obesity or a standard diet to serve as normal controls. A mean body weight increase of more than 20% after these 12 weeks was used as the criteria for obesity. HFD-fed obese mice then received a supplement of Sal B (100 mg/kg body weight/day), metformin (75 mg/kg body weight/day) or water (an equivalent volume; served as model controls) by oral gavage for an additional 8 weeks, and the normal controls received water (an equivalent volume) by oral gavage for the same period. Results: Sal B significantly reduced body weight gain (P < .05) without influencing food intake in HFD-fed obese mice relative to model controls. Sal B also reduced the body fat mass of the obese mice relative to model controls in a time-dependent manner (P < .05). Sal B significantly decreased the serum concentrations of low-density lipoprotein cholesterol, total cholesterol, triglyceride and free fatty acids by 25.5%, 20.2%, 20.6% and 13.4%, respectively, and increased the concentration of high-density lipoprotein cholesterol by 50.1% relative to model controls. In addition, Sal B significantly lowered fasting glucose concentrations and improved insulin sensitivity relative to model controls (P < .05). Sal B acted by ameliorating the histopathological changes in both brown and white adipose tissues of obese mice. Moreover, in brown adipose tissue, Sal B up-regulated the mRNA and protein expression of PPARγ and c/EBPα, and the protein expression of PPARα and SREBP-1 (P < .05). In white adipose tissue, Sal B down-regulated the mRNA expression of PPARγ and c/EBPα, and decreased the protein expression of PPARγ and SREBP-1(P < .05). Conclusions: The results suggest that Sal B can reduce body weight gain and regulate glucose and lipid metabolism in mice with diet-induced obesity by regulating adipogenic transcription factors in their adipose tissues.http://www.sciencedirect.com/science/article/pii/S2095754817300923Salvianolic acid BObesityBlood glucoseAdipose tissueAdipogenic transcription factors |
| spellingShingle | Dandan Zhao Jiacheng Zuo Na Yu Xin Fang Fangfang Mo Rui Wu Tian Tian Rufeng Ma Yushan Gao Dongwei Zhang Guangjian Jiang Sihua Gao Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity Journal of Traditional Chinese Medical Sciences Salvianolic acid B Obesity Blood glucose Adipose tissue Adipogenic transcription factors |
| title | Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity |
| title_full | Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity |
| title_fullStr | Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity |
| title_full_unstemmed | Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity |
| title_short | Salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity |
| title_sort | salvianolic acid b improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet induced obesity |
| topic | Salvianolic acid B Obesity Blood glucose Adipose tissue Adipogenic transcription factors |
| url | http://www.sciencedirect.com/science/article/pii/S2095754817300923 |
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