<i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection
<i>Smilax aspera</i> L. (commonly known as sarsaparilla) is recognized for its composition rich in flavonoids, phenylpropanoids, steroidal saponins, stilbenoids, and tannins, exhibiting anti-inflammatory, cytotoxic, and antimicrobial properties. This study investigates the hydromethanoli...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/14/2/383 |
| _version_ | 1797299181353697280 |
|---|---|
| author | Riccardo Fontana Eva Sánchez-Hernández Pablo Martín-Ramos Jesús Martín-Gil Peggy Marconi |
| author_facet | Riccardo Fontana Eva Sánchez-Hernández Pablo Martín-Ramos Jesús Martín-Gil Peggy Marconi |
| author_sort | Riccardo Fontana |
| collection | DOAJ |
| description | <i>Smilax aspera</i> L. (commonly known as sarsaparilla) is recognized for its composition rich in flavonoids, phenylpropanoids, steroidal saponins, stilbenoids, and tannins, exhibiting anti-inflammatory, cytotoxic, and antimicrobial properties. This study investigates the hydromethanolic extracts of its leaves and fruits through vibrational spectroscopy and gas chromatography–mass spectrometry, evaluating their potential as biorationals for safeguarding crops. Analysis of <i>S. aspera</i> leaf and fruit extracts revealed the presence of phytochemicals such as lactones and other furan derivatives. In vitro assessments against three phytopathogens—<i>Erwinia amylovora</i>, <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>, and <i>Xanthomonas campestris</i> pv. <i>campestris</i>—demonstrated strong antibacterial activity, with minimum inhibitory concentration (MIC) values of 1500 μg·mL<sup>−1</sup> for both extracts. Biofilm tests indicated that the leaf extract reduced biofilm formation by 78–85%, while the fruit extract led to a reduction of 73–92.5%. At a concentration of 750 µg·mL<sup>−1</sup>, the extracts caused a decrease in amylovoran synthesis by 41–58%. Additionally, noticeable alterations in membrane permeability were observed at MIC and MIC/2 doses. Subsequent in vivo trials conducted on <i>Pyrus communis</i> L. trees utilizing the combined aerial part extract yielded substantial protection against <i>E. amylovora</i> at a dose of 1500 μg·mL<sup>−1</sup>, reaching 80% wilting reduction for the leaf extract. The findings presented herein cast <i>S. aspera</i> extracts as a promising natural-based treatment against these bacterial phytopathogens. |
| first_indexed | 2024-03-07T22:45:59Z |
| format | Article |
| id | doaj.art-c9bdddd5012d4ea281974d2bc3e08209 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-07T22:45:59Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c9bdddd5012d4ea281974d2bc3e082092024-02-23T15:04:22ZengMDPI AGAgronomy2073-43952024-02-0114238310.3390/agronomy14020383<i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop ProtectionRiccardo Fontana0Eva Sánchez-Hernández1Pablo Martín-Ramos2Jesús Martín-Gil3Peggy Marconi4Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121 Ferrara, ItalyDepartment of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, 34004 Palencia, SpainDepartment of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, 34004 Palencia, SpainDepartment of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, 34004 Palencia, SpainDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121 Ferrara, Italy<i>Smilax aspera</i> L. (commonly known as sarsaparilla) is recognized for its composition rich in flavonoids, phenylpropanoids, steroidal saponins, stilbenoids, and tannins, exhibiting anti-inflammatory, cytotoxic, and antimicrobial properties. This study investigates the hydromethanolic extracts of its leaves and fruits through vibrational spectroscopy and gas chromatography–mass spectrometry, evaluating their potential as biorationals for safeguarding crops. Analysis of <i>S. aspera</i> leaf and fruit extracts revealed the presence of phytochemicals such as lactones and other furan derivatives. In vitro assessments against three phytopathogens—<i>Erwinia amylovora</i>, <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>, and <i>Xanthomonas campestris</i> pv. <i>campestris</i>—demonstrated strong antibacterial activity, with minimum inhibitory concentration (MIC) values of 1500 μg·mL<sup>−1</sup> for both extracts. Biofilm tests indicated that the leaf extract reduced biofilm formation by 78–85%, while the fruit extract led to a reduction of 73–92.5%. At a concentration of 750 µg·mL<sup>−1</sup>, the extracts caused a decrease in amylovoran synthesis by 41–58%. Additionally, noticeable alterations in membrane permeability were observed at MIC and MIC/2 doses. Subsequent in vivo trials conducted on <i>Pyrus communis</i> L. trees utilizing the combined aerial part extract yielded substantial protection against <i>E. amylovora</i> at a dose of 1500 μg·mL<sup>−1</sup>, reaching 80% wilting reduction for the leaf extract. The findings presented herein cast <i>S. aspera</i> extracts as a promising natural-based treatment against these bacterial phytopathogens.https://www.mdpi.com/2073-4395/14/2/383antibacterialbacterial diseasesblack rot diseasecommon smilaxfire blightnatural products |
| spellingShingle | Riccardo Fontana Eva Sánchez-Hernández Pablo Martín-Ramos Jesús Martín-Gil Peggy Marconi <i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection Agronomy antibacterial bacterial diseases black rot disease common smilax fire blight natural products |
| title | <i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection |
| title_full | <i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection |
| title_fullStr | <i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection |
| title_full_unstemmed | <i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection |
| title_short | <i>Smilax aspera</i> L. Leaf and Fruit Extracts as Antibacterial Agents for Crop Protection |
| title_sort | i smilax aspera i l leaf and fruit extracts as antibacterial agents for crop protection |
| topic | antibacterial bacterial diseases black rot disease common smilax fire blight natural products |
| url | https://www.mdpi.com/2073-4395/14/2/383 |
| work_keys_str_mv | AT riccardofontana ismilaxasperailleafandfruitextractsasantibacterialagentsforcropprotection AT evasanchezhernandez ismilaxasperailleafandfruitextractsasantibacterialagentsforcropprotection AT pablomartinramos ismilaxasperailleafandfruitextractsasantibacterialagentsforcropprotection AT jesusmartingil ismilaxasperailleafandfruitextractsasantibacterialagentsforcropprotection AT peggymarconi ismilaxasperailleafandfruitextractsasantibacterialagentsforcropprotection |