Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration
Neuronal and glial activity are dependent on the efflux of potassium ions into the extracellular space. Efflux of K is partly energy-dependent as the activity of pumps and channels which are involved in K transportation is ATP-dependent. In this study, we investigated the effect of decreased intrace...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/7/1568 |
| _version_ | 1827597894493929472 |
|---|---|
| author | Azin EbrahimAmini Bojana Stefanovic Peter L. Carlen |
| author_facet | Azin EbrahimAmini Bojana Stefanovic Peter L. Carlen |
| author_sort | Azin EbrahimAmini |
| collection | DOAJ |
| description | Neuronal and glial activity are dependent on the efflux of potassium ions into the extracellular space. Efflux of K is partly energy-dependent as the activity of pumps and channels which are involved in K transportation is ATP-dependent. In this study, we investigated the effect of decreased intracellular ATP concentration ([ATP]i) on the extracellular potassium ion concentration ([K]o). Using in vivo electrophysiological techniques, we measured neocortical [K]o and the local field potential (LFP) while [ATP]i was reduced through various pharmacological interventions. We observed that reducing [ATP]i led to raised [K]o and DC-shifts resembling spreading depolarization-like events. We proposed that most likely, the increased [K]o is mainly due to the impairment of the Na/K ATPase pump and the ATP-sensitive potassium channel in the absence of sufficient ATP, because Na/K ATPase inhibition led to increased [K]o and ATP-sensitive potassium channel impairment resulted in decreased [K]o. Therefore, an important consequence of decreased [ATP]i is an increased [K]o. The results of this study acknowledge one of the mechanisms involved in [K]o dynamics. |
| first_indexed | 2024-03-09T03:40:48Z |
| format | Article |
| id | doaj.art-c9be6870205a4512b91cb63414c24ae2 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T03:40:48Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c9be6870205a4512b91cb63414c24ae22023-12-03T14:41:38ZengMDPI AGBiomedicines2227-90592022-07-01107156810.3390/biomedicines10071568Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium ConcentrationAzin EbrahimAmini0Bojana Stefanovic1Peter L. Carlen2Krembil Research Institute, Toronto, ON M5T 0S8, CanadaSunnybrook Health Sciences Center, Medical Biophysics, Toronto, ON M4N 3M5, CanadaKrembil Research Institute, Toronto, ON M5T 0S8, CanadaNeuronal and glial activity are dependent on the efflux of potassium ions into the extracellular space. Efflux of K is partly energy-dependent as the activity of pumps and channels which are involved in K transportation is ATP-dependent. In this study, we investigated the effect of decreased intracellular ATP concentration ([ATP]i) on the extracellular potassium ion concentration ([K]o). Using in vivo electrophysiological techniques, we measured neocortical [K]o and the local field potential (LFP) while [ATP]i was reduced through various pharmacological interventions. We observed that reducing [ATP]i led to raised [K]o and DC-shifts resembling spreading depolarization-like events. We proposed that most likely, the increased [K]o is mainly due to the impairment of the Na/K ATPase pump and the ATP-sensitive potassium channel in the absence of sufficient ATP, because Na/K ATPase inhibition led to increased [K]o and ATP-sensitive potassium channel impairment resulted in decreased [K]o. Therefore, an important consequence of decreased [ATP]i is an increased [K]o. The results of this study acknowledge one of the mechanisms involved in [K]o dynamics.https://www.mdpi.com/2227-9059/10/7/1568extracellular potassium concentrationintracellular ATP concentrationNa/K ATPase pumpATP-sensitive potassium channel |
| spellingShingle | Azin EbrahimAmini Bojana Stefanovic Peter L. Carlen Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration Biomedicines extracellular potassium concentration intracellular ATP concentration Na/K ATPase pump ATP-sensitive potassium channel |
| title | Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration |
| title_full | Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration |
| title_fullStr | Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration |
| title_full_unstemmed | Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration |
| title_short | Effects of In Vivo Intracellular ATP Modulation on Neocortical Extracellular Potassium Concentration |
| title_sort | effects of in vivo intracellular atp modulation on neocortical extracellular potassium concentration |
| topic | extracellular potassium concentration intracellular ATP concentration Na/K ATPase pump ATP-sensitive potassium channel |
| url | https://www.mdpi.com/2227-9059/10/7/1568 |
| work_keys_str_mv | AT azinebrahimamini effectsofinvivointracellularatpmodulationonneocorticalextracellularpotassiumconcentration AT bojanastefanovic effectsofinvivointracellularatpmodulationonneocorticalextracellularpotassiumconcentration AT peterlcarlen effectsofinvivointracellularatpmodulationonneocorticalextracellularpotassiumconcentration |