Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D
Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity dec...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2008-08-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007 |
| _version_ | 1828553192098496512 |
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| author | P.H. Rocha R.A. Assis F.C.F. Lobato V.N. Cardoso L.G.D. Heneine |
| author_facet | P.H. Rocha R.A. Assis F.C.F. Lobato V.N. Cardoso L.G.D. Heneine |
| author_sort | P.H. Rocha |
| collection | DOAJ |
| description | Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.<br>A prototoxina epsílon de Clostridium perfringens tipo D foi produzida, purificada e destoxificada por estoiquiometria. Diferentes quantidades de iodo foram incorporadas à estrutura protéica da prototoxina e a toxicidade dos derivados foi analisada em estudos in vivo. Verificou-se que a toxicidade diminuiu à medida que os átomos de iodo foram incorporados à prototoxina. A análise eletroforética demonstrou a estabilidade das amostras quando armazenadas a 4ºC, -20ºC e -80ºC. A prototoxina iodada foi estocada por um período de quatro meses. |
| first_indexed | 2024-12-12T05:13:05Z |
| format | Article |
| id | doaj.art-c9c1cf897c464544b46bafbcef8ba4e3 |
| institution | Directory Open Access Journal |
| issn | 0102-0935 1678-4162 |
| language | English |
| last_indexed | 2024-12-12T05:13:05Z |
| publishDate | 2008-08-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-c9c1cf897c464544b46bafbcef8ba4e32022-12-22T00:36:50ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia0102-09351678-41622008-08-0160482182410.1590/S0102-09352008000400007Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo DP.H. RochaR.A. AssisF.C.F. LobatoV.N. CardosoL.G.D. HeneinePurified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.<br>A prototoxina epsílon de Clostridium perfringens tipo D foi produzida, purificada e destoxificada por estoiquiometria. Diferentes quantidades de iodo foram incorporadas à estrutura protéica da prototoxina e a toxicidade dos derivados foi analisada em estudos in vivo. Verificou-se que a toxicidade diminuiu à medida que os átomos de iodo foram incorporados à prototoxina. A análise eletroforética demonstrou a estabilidade das amostras quando armazenadas a 4ºC, -20ºC e -80ºC. A prototoxina iodada foi estocada por um período de quatro meses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007Clostridium perfringenstoxina epsíloniodaçãoestabilidadeClostridium perfringensepsilon toxiniodinationstability |
| spellingShingle | P.H. Rocha R.A. Assis F.C.F. Lobato V.N. Cardoso L.G.D. Heneine Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D Arquivo Brasileiro de Medicina Veterinária e Zootecnia Clostridium perfringens toxina epsílon iodação estabilidade Clostridium perfringens epsilon toxin iodination stability |
| title | Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D |
| title_full | Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D |
| title_fullStr | Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D |
| title_full_unstemmed | Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D |
| title_short | Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D |
| title_sort | stability and toxicity of clostridium perfringens type d epsilon prototoxin treated by iodine estabilidade e toxicidade da protoxina epsilon de clostridium perfringens tipo d |
| topic | Clostridium perfringens toxina epsílon iodação estabilidade Clostridium perfringens epsilon toxin iodination stability |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007 |
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