Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections
There is an alarming rise in carbapenem-resistant Enterobacteriaceae (CRE) causing nosocomial infections such as ventilator-associated respiratory infections (VARIs). The use of rapid phenotypic methods for the detection and differentiation of carbapenemases elaborated by these CRE would be helpful...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2022-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/use-of-the-combined-modified-carbapenem-inactivation-method-and-edta-modified-carbapenem-inactivation-method-for-detection-of-carbapenemase-producing-enterobacteriaceae-causing-ventilator-associated-r/ |
| _version_ | 1818469053339009024 |
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| author | Nupur Koul Barnali Kakati Sonika Agarwal |
| author_facet | Nupur Koul Barnali Kakati Sonika Agarwal |
| author_sort | Nupur Koul |
| collection | DOAJ |
| description | There is an alarming rise in carbapenem-resistant Enterobacteriaceae (CRE) causing nosocomial infections such as ventilator-associated respiratory infections (VARIs). The use of rapid phenotypic methods for the detection and differentiation of carbapenemases elaborated by these CRE would be helpful in providing timely empirical therapeutic options for management of these infections and preventing spread of these CRE strains in hospital settings. Hence, this study aimed to detect CRE among pathogens isolated from the endotracheal secretions recieved from suspected cases of VARIs and differentiate carbapenemases elaborated by these CRE using combined phenotypic methods, such as the modified carbapenem inactivation method (mCIM) and EDTA modified CIM (eCIM). This observational study was conducted over a period of 1 year in the Department of Microbiology and the intensive care unit of a tertiary care center. Carbapenem resistance was found in 75% of Klebsiella pneumoniae isolates and 50% of Escherichia coli isolates, of which 58.4% were metallo-β-lactamases and 41.6% were serine carbapenemase producers. In conclusion, the combination of the mCIM and eCIM could be useful as an epidemiological tool and be considered essential in deciding the initial antibiotic therapy, help reduce morbidity and mortality associated with VARIs, and guide hospital infection control practices. |
| first_indexed | 2024-04-13T21:18:59Z |
| format | Article |
| id | doaj.art-c9c5335989d74c648669390ab452e9a0 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-13T21:18:59Z |
| publishDate | 2022-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-c9c5335989d74c648669390ab452e9a02022-12-22T02:29:34ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-06-0116212391244https://doi.org/10.22207/JPAM.16.2.52Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory InfectionsNupur Koulhttps://orcid.org/0000-0002-3652-5584Barnali Kakatihttps://orcid.org/0000-0002-1592-9109Sonika AgarwalThere is an alarming rise in carbapenem-resistant Enterobacteriaceae (CRE) causing nosocomial infections such as ventilator-associated respiratory infections (VARIs). The use of rapid phenotypic methods for the detection and differentiation of carbapenemases elaborated by these CRE would be helpful in providing timely empirical therapeutic options for management of these infections and preventing spread of these CRE strains in hospital settings. Hence, this study aimed to detect CRE among pathogens isolated from the endotracheal secretions recieved from suspected cases of VARIs and differentiate carbapenemases elaborated by these CRE using combined phenotypic methods, such as the modified carbapenem inactivation method (mCIM) and EDTA modified CIM (eCIM). This observational study was conducted over a period of 1 year in the Department of Microbiology and the intensive care unit of a tertiary care center. Carbapenem resistance was found in 75% of Klebsiella pneumoniae isolates and 50% of Escherichia coli isolates, of which 58.4% were metallo-β-lactamases and 41.6% were serine carbapenemase producers. In conclusion, the combination of the mCIM and eCIM could be useful as an epidemiological tool and be considered essential in deciding the initial antibiotic therapy, help reduce morbidity and mortality associated with VARIs, and guide hospital infection control practices.https://microbiologyjournal.org/use-of-the-combined-modified-carbapenem-inactivation-method-and-edta-modified-carbapenem-inactivation-method-for-detection-of-carbapenemase-producing-enterobacteriaceae-causing-ventilator-associated-r/modified carbapenem inactivation methodedta-modified cimcarbapenemase-producing enterobacteriaceaeventilator-associated respiratory infections |
| spellingShingle | Nupur Koul Barnali Kakati Sonika Agarwal Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections Journal of Pure and Applied Microbiology modified carbapenem inactivation method edta-modified cim carbapenemase-producing enterobacteriaceae ventilator-associated respiratory infections |
| title | Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections |
| title_full | Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections |
| title_fullStr | Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections |
| title_full_unstemmed | Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections |
| title_short | Use of the Combined Modified Carbapenem Inactivation Method and EDTA-modified Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacteriaceae Causing Ventilator-associated Respiratory Infections |
| title_sort | use of the combined modified carbapenem inactivation method and edta modified carbapenem inactivation method for detection of carbapenemase producing enterobacteriaceae causing ventilator associated respiratory infections |
| topic | modified carbapenem inactivation method edta-modified cim carbapenemase-producing enterobacteriaceae ventilator-associated respiratory infections |
| url | https://microbiologyjournal.org/use-of-the-combined-modified-carbapenem-inactivation-method-and-edta-modified-carbapenem-inactivation-method-for-detection-of-carbapenemase-producing-enterobacteriaceae-causing-ventilator-associated-r/ |
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