Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain
The objectives of this study were to investigate the prevalence and fluoroquinolone resistant Salmonella isolated from an integrated broiler chicken supply chain and their molecular characterization. In total, 73 Salmonella isolates were recovered from a broiler chicken supply chain in Shanghai. Sal...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01865/full |
| _version_ | 1811213435863564288 |
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| author | Mingquan Cui Peng Zhang Peng Zhang Jiyun Li Jiyun Li Chengtao Sun Chengtao Sun Li Song Chunping Zhang Qi Zhao Congming Wu Congming Wu |
| author_facet | Mingquan Cui Peng Zhang Peng Zhang Jiyun Li Jiyun Li Chengtao Sun Chengtao Sun Li Song Chunping Zhang Qi Zhao Congming Wu Congming Wu |
| author_sort | Mingquan Cui |
| collection | DOAJ |
| description | The objectives of this study were to investigate the prevalence and fluoroquinolone resistant Salmonella isolated from an integrated broiler chicken supply chain and their molecular characterization. In total, 73 Salmonella isolates were recovered from a broiler chicken supply chain in Shanghai. Salmonella isolates were tested for susceptibility to 11 antimicrobial agents using the broth dilution method and were characterized using pulsed-field gel electrophoresis (PFGE). Then, the Salmonella isolates were examined for mutations in quinolone resistance-determining region (QRDR) of gyrA, gyrB, parC, and parE, and were screened for plasmid-mediated quinolone resistance (PMQR) genes. Lastly, we sequenced the plasmids carrying qnrS1 in six Salmonella isolates from three sources (two isolated per source). Among 73 Salmonella isolates, 45 isolates were identified as S. Indiana, 24 were S. Schwarzengrund, 2 were S. Enteritidis, and 2 were S. Stanleyville. In addition, high rates of resistance were detected for nalidixic acid (41.1%) and ciprofloxacin (37.0%), while resistance to other test agents was diverse (2.0–100%). S. Indiana and S. Schwarzengrund isolates from different sources exhibited the same PFGE pattern, suggesting that the Salmonella isolates possessed high potential to spread along the broiler chicken supply chain. gyrA and parC exhibited frequent missense mutations. Moreover, qnrS1 was the most prevalent PMQR gene in the 73 Salmonella isolates, and it was found about a new hybrid plasmid. This study concludes a high prevalence of fluoroquinolone resistant Salmonella in chicken supply chain, threatening the treatment of Salmonella foodborne diseases. In particular, the emergence of a new hybrid plasmid carrying qnrS1 indicates that the recombination of plasmid carrying resistance gene might be a potential risk factor for the prevention and control strategies of drug resistance. |
| first_indexed | 2024-04-12T05:45:40Z |
| format | Article |
| id | doaj.art-c9c71839e3734d1d951405732cdbb674 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T05:45:40Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c9c71839e3734d1d951405732cdbb6742022-12-22T03:45:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-08-011010.3389/fmicb.2019.01865418303Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply ChainMingquan Cui0Peng Zhang1Peng Zhang2Jiyun Li3Jiyun Li4Chengtao Sun5Chengtao Sun6Li Song7Chunping Zhang8Qi Zhao9Congming Wu10Congming Wu11China Institute of Veterinary Drug Control, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, MOA, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, MOA, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, MOA, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaChina Institute of Veterinary Drug Control, Beijing, ChinaChina Institute of Veterinary Drug Control, Beijing, ChinaChina Institute of Veterinary Drug Control, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, MOA, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaThe objectives of this study were to investigate the prevalence and fluoroquinolone resistant Salmonella isolated from an integrated broiler chicken supply chain and their molecular characterization. In total, 73 Salmonella isolates were recovered from a broiler chicken supply chain in Shanghai. Salmonella isolates were tested for susceptibility to 11 antimicrobial agents using the broth dilution method and were characterized using pulsed-field gel electrophoresis (PFGE). Then, the Salmonella isolates were examined for mutations in quinolone resistance-determining region (QRDR) of gyrA, gyrB, parC, and parE, and were screened for plasmid-mediated quinolone resistance (PMQR) genes. Lastly, we sequenced the plasmids carrying qnrS1 in six Salmonella isolates from three sources (two isolated per source). Among 73 Salmonella isolates, 45 isolates were identified as S. Indiana, 24 were S. Schwarzengrund, 2 were S. Enteritidis, and 2 were S. Stanleyville. In addition, high rates of resistance were detected for nalidixic acid (41.1%) and ciprofloxacin (37.0%), while resistance to other test agents was diverse (2.0–100%). S. Indiana and S. Schwarzengrund isolates from different sources exhibited the same PFGE pattern, suggesting that the Salmonella isolates possessed high potential to spread along the broiler chicken supply chain. gyrA and parC exhibited frequent missense mutations. Moreover, qnrS1 was the most prevalent PMQR gene in the 73 Salmonella isolates, and it was found about a new hybrid plasmid. This study concludes a high prevalence of fluoroquinolone resistant Salmonella in chicken supply chain, threatening the treatment of Salmonella foodborne diseases. In particular, the emergence of a new hybrid plasmid carrying qnrS1 indicates that the recombination of plasmid carrying resistance gene might be a potential risk factor for the prevention and control strategies of drug resistance.https://www.frontiersin.org/article/10.3389/fmicb.2019.01865/fullSalmonellafluoroquinolone resistanceqnrS1hybrid plasmidbroiler chicken supply chain |
| spellingShingle | Mingquan Cui Peng Zhang Peng Zhang Jiyun Li Jiyun Li Chengtao Sun Chengtao Sun Li Song Chunping Zhang Qi Zhao Congming Wu Congming Wu Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain Frontiers in Microbiology Salmonella fluoroquinolone resistance qnrS1 hybrid plasmid broiler chicken supply chain |
| title | Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain |
| title_full | Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain |
| title_fullStr | Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain |
| title_full_unstemmed | Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain |
| title_short | Prevalence and Characterization of Fluoroquinolone Resistant Salmonella Isolated From an Integrated Broiler Chicken Supply Chain |
| title_sort | prevalence and characterization of fluoroquinolone resistant salmonella isolated from an integrated broiler chicken supply chain |
| topic | Salmonella fluoroquinolone resistance qnrS1 hybrid plasmid broiler chicken supply chain |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01865/full |
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