Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique
Tuberculosis (TB) is considered as one of the most important infectious diseases in the world, and recent rise and spread of multidrug-resistant (MDR) Mycobacterium tuberculosis (MTB) strains, have made the matter worsened. Due to the importance of TB prevalence in Iran, this study was designed to i...
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| Format: | Article |
| Language: | English |
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Wiley
2017-11-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X17300566 |
| _version_ | 1818888186125877248 |
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| author | Azar Dokht Khosravi Abdolrazagh Hashemi Shahraki Soolmaz Khandan Dezfuli Mohammad Hashemzadeh Hamed Goodarzi Parviz Mohajeri |
| author_facet | Azar Dokht Khosravi Abdolrazagh Hashemi Shahraki Soolmaz Khandan Dezfuli Mohammad Hashemzadeh Hamed Goodarzi Parviz Mohajeri |
| author_sort | Azar Dokht Khosravi |
| collection | DOAJ |
| description | Tuberculosis (TB) is considered as one of the most important infectious diseases in the world, and recent rise and spread of multidrug-resistant (MDR) Mycobacterium tuberculosis (MTB) strains, have made the matter worsened. Due to the importance of TB prevalence in Iran, this study was designed to investigate the genetic diversity among MDR strains of MTB by MIRU-VNTR typing scheme. A total of 88 drug resistant M. tuberculosis isolates belong to pulmonary TB cases were collected from several TB reference centers of Iran. Drug susceptibility testing for Isoniazid and Rifampin was performed using the agar proportion method and MDR isolates were underwent genotyping by using 12-locus- based MIRU-VNTR typing. On performing proportion method, 22 isolates were identified as MDR. By typing of MDR isolates using 12-loci MIRU-VNTR technique, high diversity were demonstrated in MDR strains and these were classified into 20 distinct MIRU-VNTR genotypes. MIRU loci 10 and 26 were the most discriminatory loci with 8 and 7 alleles respectively; while MIRU loci 2, 20, 24 and 39 were found to be the least discriminatory with 1–2 alleles each. We noticed a mixed infection in isolate 53, as this isolate comprised simultaneous two alleles in MIRU loci 40, 10, 16 and 39. In conclusion, this result represents MIRU-VNTR typing as a useful tool for studying genetic diversity of MDR-MTB in regional settings, and will help the health sectors to construct a preventive program for MDR-TB. Additionally, it can detect mixed infection which can facilitate management of treatment. |
| first_indexed | 2024-12-19T16:49:07Z |
| format | Article |
| id | doaj.art-c9c88c0db5384550a167a3c3c19b34f2 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-19T16:49:07Z |
| publishDate | 2017-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-c9c88c0db5384550a167a3c3c19b34f22022-12-21T20:13:35ZengWileyKaohsiung Journal of Medical Sciences1607-551X2017-11-01331155055710.1016/j.kjms.2017.06.011Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR techniqueAzar Dokht Khosravi0Abdolrazagh Hashemi Shahraki1Soolmaz Khandan Dezfuli2Mohammad Hashemzadeh3Hamed Goodarzi4Parviz Mohajeri5Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Epidemiology, Pasteur Institute of Iran, Tehran, IranDepartment of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranTuberculosis (TB) is considered as one of the most important infectious diseases in the world, and recent rise and spread of multidrug-resistant (MDR) Mycobacterium tuberculosis (MTB) strains, have made the matter worsened. Due to the importance of TB prevalence in Iran, this study was designed to investigate the genetic diversity among MDR strains of MTB by MIRU-VNTR typing scheme. A total of 88 drug resistant M. tuberculosis isolates belong to pulmonary TB cases were collected from several TB reference centers of Iran. Drug susceptibility testing for Isoniazid and Rifampin was performed using the agar proportion method and MDR isolates were underwent genotyping by using 12-locus- based MIRU-VNTR typing. On performing proportion method, 22 isolates were identified as MDR. By typing of MDR isolates using 12-loci MIRU-VNTR technique, high diversity were demonstrated in MDR strains and these were classified into 20 distinct MIRU-VNTR genotypes. MIRU loci 10 and 26 were the most discriminatory loci with 8 and 7 alleles respectively; while MIRU loci 2, 20, 24 and 39 were found to be the least discriminatory with 1–2 alleles each. We noticed a mixed infection in isolate 53, as this isolate comprised simultaneous two alleles in MIRU loci 40, 10, 16 and 39. In conclusion, this result represents MIRU-VNTR typing as a useful tool for studying genetic diversity of MDR-MTB in regional settings, and will help the health sectors to construct a preventive program for MDR-TB. Additionally, it can detect mixed infection which can facilitate management of treatment.http://www.sciencedirect.com/science/article/pii/S1607551X17300566Mycobacterium tuberculosisMultidrug resistantMIRU-VNTRTuberculosis |
| spellingShingle | Azar Dokht Khosravi Abdolrazagh Hashemi Shahraki Soolmaz Khandan Dezfuli Mohammad Hashemzadeh Hamed Goodarzi Parviz Mohajeri Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique Kaohsiung Journal of Medical Sciences Mycobacterium tuberculosis Multidrug resistant MIRU-VNTR Tuberculosis |
| title | Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique |
| title_full | Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique |
| title_fullStr | Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique |
| title_full_unstemmed | Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique |
| title_short | Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique |
| title_sort | genetic diversity of multidrug resistant mycobacterium tuberculosis strains isolated from tuberculosis patients in iran using miru vntr technique |
| topic | Mycobacterium tuberculosis Multidrug resistant MIRU-VNTR Tuberculosis |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X17300566 |
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