UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic.
Personal protective equipment (PPE) is crucially important to the safety of both patients and medical personnel, particularly in the event of an infectious pandemic. As the incidence of Coronavirus Disease 2019 (COVID-19) increases exponentially in the United States and many parts of the world, heal...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0241734 |
| _version_ | 1818195248013115392 |
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| author | Davis T Weaver Benjamin D McElvany Vishhvaan Gopalakrishnan Kyle J Card Dena Crozier Andrew Dhawan Mina N Dinh Emily Dolson Nathan Farrokhian Masahiro Hitomi Emily Ho Tanush Jagdish Eshan S King Jennifer L Cadnum Curtis J Donskey Nikhil Krishnan Gleb Kuzmin Ju Li Jeff Maltas Jinhan Mo Julia Pelesko Jessica A Scarborough Geoff Sedor Enze Tian Gary C An Sean A Diehl Jacob G Scott |
| author_facet | Davis T Weaver Benjamin D McElvany Vishhvaan Gopalakrishnan Kyle J Card Dena Crozier Andrew Dhawan Mina N Dinh Emily Dolson Nathan Farrokhian Masahiro Hitomi Emily Ho Tanush Jagdish Eshan S King Jennifer L Cadnum Curtis J Donskey Nikhil Krishnan Gleb Kuzmin Ju Li Jeff Maltas Jinhan Mo Julia Pelesko Jessica A Scarborough Geoff Sedor Enze Tian Gary C An Sean A Diehl Jacob G Scott |
| author_sort | Davis T Weaver |
| collection | DOAJ |
| description | Personal protective equipment (PPE) is crucially important to the safety of both patients and medical personnel, particularly in the event of an infectious pandemic. As the incidence of Coronavirus Disease 2019 (COVID-19) increases exponentially in the United States and many parts of the world, healthcare provider demand for these necessities is currently outpacing supply. In the midst of the current pandemic, there has been a concerted effort to identify viable ways to conserve PPE, including decontamination after use. In this study, we outline a procedure by which PPE may be decontaminated using ultraviolet (UV) radiation in biosafety cabinets (BSCs), a common element of many academic, public health, and hospital laboratories. According to the literature, effective decontamination of N95 respirator masks or surgical masks requires UV-C doses of greater than 1 Jcm-2, which was achieved after 4.3 hours per side when placing the N95 at the bottom of the BSCs tested in this study. We then demonstrated complete inactivation of the human coronavirus NL63 on N95 mask material after 15 minutes of UV-C exposure at 61 cm (232 μWcm-2). Our results provide support to healthcare organizations looking for methods to extend their reserves of PPE. |
| first_indexed | 2024-12-12T01:15:10Z |
| format | Article |
| id | doaj.art-c9cb9eb3a2b1444ea76753df9db95399 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T01:15:10Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c9cb9eb3a2b1444ea76753df9db953992022-12-22T00:43:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01167e024173410.1371/journal.pone.0241734UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic.Davis T WeaverBenjamin D McElvanyVishhvaan GopalakrishnanKyle J CardDena CrozierAndrew DhawanMina N DinhEmily DolsonNathan FarrokhianMasahiro HitomiEmily HoTanush JagdishEshan S KingJennifer L CadnumCurtis J DonskeyNikhil KrishnanGleb KuzminJu LiJeff MaltasJinhan MoJulia PeleskoJessica A ScarboroughGeoff SedorEnze TianGary C AnSean A DiehlJacob G ScottPersonal protective equipment (PPE) is crucially important to the safety of both patients and medical personnel, particularly in the event of an infectious pandemic. As the incidence of Coronavirus Disease 2019 (COVID-19) increases exponentially in the United States and many parts of the world, healthcare provider demand for these necessities is currently outpacing supply. In the midst of the current pandemic, there has been a concerted effort to identify viable ways to conserve PPE, including decontamination after use. In this study, we outline a procedure by which PPE may be decontaminated using ultraviolet (UV) radiation in biosafety cabinets (BSCs), a common element of many academic, public health, and hospital laboratories. According to the literature, effective decontamination of N95 respirator masks or surgical masks requires UV-C doses of greater than 1 Jcm-2, which was achieved after 4.3 hours per side when placing the N95 at the bottom of the BSCs tested in this study. We then demonstrated complete inactivation of the human coronavirus NL63 on N95 mask material after 15 minutes of UV-C exposure at 61 cm (232 μWcm-2). Our results provide support to healthcare organizations looking for methods to extend their reserves of PPE.https://doi.org/10.1371/journal.pone.0241734 |
| spellingShingle | Davis T Weaver Benjamin D McElvany Vishhvaan Gopalakrishnan Kyle J Card Dena Crozier Andrew Dhawan Mina N Dinh Emily Dolson Nathan Farrokhian Masahiro Hitomi Emily Ho Tanush Jagdish Eshan S King Jennifer L Cadnum Curtis J Donskey Nikhil Krishnan Gleb Kuzmin Ju Li Jeff Maltas Jinhan Mo Julia Pelesko Jessica A Scarborough Geoff Sedor Enze Tian Gary C An Sean A Diehl Jacob G Scott UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic. PLoS ONE |
| title | UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic. |
| title_full | UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic. |
| title_fullStr | UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic. |
| title_full_unstemmed | UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic. |
| title_short | UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic. |
| title_sort | uv decontamination of personal protective equipment with idle laboratory biosafety cabinets during the covid 19 pandemic |
| url | https://doi.org/10.1371/journal.pone.0241734 |
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