Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues
Shuyun Zhang,1,* Lijuan Wu,1,* Xue Li,1 Li Yang,1 Sujuan Shen,1 Hong Shen,1 Dan Zhao,2 Lin Qi2,3 1Center of Reproductive Medicine, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China; 2Precision Medicine Center, The Second Affiliated Hospital of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2023-11-01
|
| Series: | International Journal of Women's Health |
| Subjects: | |
| Online Access: | https://www.dovepress.com/clinical-significance-of-application-of-chromosomal-karyotyping-of-vil-peer-reviewed-fulltext-article-IJWH |
| _version_ | 1797634248732049408 |
|---|---|
| author | Zhang S Wu L Li X Yang L Shen S Shen H Zhao D Qi L |
| author_facet | Zhang S Wu L Li X Yang L Shen S Shen H Zhao D Qi L |
| author_sort | Zhang S |
| collection | DOAJ |
| description | Shuyun Zhang,1,* Lijuan Wu,1,* Xue Li,1 Li Yang,1 Sujuan Shen,1 Hong Shen,1 Dan Zhao,2 Lin Qi2,3 1Center of Reproductive Medicine, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China; 2Precision Medicine Center, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China; 3State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, 215000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lin Qi, Precision Medicine Center, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China, Tel +86-13962187070, Email drhuan@126.comPurpose: Karyotype analysis of villus chromosomes in spontaneous abortion by high-throughput ligation-dependent probe amplification (HLPA) was performed to explore relationship between spontaneous abortion and chromosomal abnormalities in spontaneous abortion tissues.Patients and Methods: The karyotypes of chromosomes of villus tissues from 516 patients with spontaneous abortion who were admitted to our hospital between 2014 and 2019 were analyzed. The data were grouped by the age (ie, ≤ 29, 30– 35, and ≥ 36 years old) in which embryonic chromosomal abnormality rate, and abnormal distribution of chromosome number in abortion tissue of 294 patients.Results: The examination of 516 samples of spontaneous abortion tissues showed that the chromosomes of 294 samples were abnormal, and the abnormality rate was 60.97%. Among 294 samples with chromosomal abnormalities, 136 (89.47%) samples had numerical chromosomal abnormalities, 15 (9.87%) samples had structural chromosomal abnormalities, and 1 (0.07%) sample had uniparental disomy. A total of 308 couples voluntarily underwent peripheral blood chromosome analysis. The results showed that 56 couples had abnormality in one or both spouses, and rate of embryonic chromosomal abnormality in these 26 cases with chromosomal abnormalities was 100%.Conclusion: Numerical and structural chromosomal abnormalities are among the important causes of embryonic arrest, while the increase in age could also be a high-risk factor for embryonic chromosomal abnormality. Therefore, examination of the karyotypes of embryo chorionic villus may help understand the reasons of embryonic arrest, which could provide important guidance for more genetic counseling for patients with early spontaneous abortion, as well as improving the preconception preparation.Keywords: spontaneous abortion, villus tissue, HLPA test, chromosomal abnormality |
| first_indexed | 2024-03-11T12:05:05Z |
| format | Article |
| id | doaj.art-c9cc854b9255464e9a3413f76eeb8981 |
| institution | Directory Open Access Journal |
| issn | 1179-1411 |
| language | English |
| last_indexed | 2024-03-11T12:05:05Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Women's Health |
| spelling | doaj.art-c9cc854b9255464e9a3413f76eeb89812023-11-07T17:06:36ZengDove Medical PressInternational Journal of Women's Health1179-14112023-11-01Volume 151705171087952Clinical Significance of Application of Chromosomal Karyotyping of Villus TissuesZhang SWu LLi XYang LShen SShen HZhao DQi LShuyun Zhang,1,* Lijuan Wu,1,* Xue Li,1 Li Yang,1 Sujuan Shen,1 Hong Shen,1 Dan Zhao,2 Lin Qi2,3 1Center of Reproductive Medicine, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China; 2Precision Medicine Center, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China; 3State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, 215000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lin Qi, Precision Medicine Center, The Second Affiliated Hospital of Soochow University, Su Zhou, People’s Republic of China, Tel +86-13962187070, Email drhuan@126.comPurpose: Karyotype analysis of villus chromosomes in spontaneous abortion by high-throughput ligation-dependent probe amplification (HLPA) was performed to explore relationship between spontaneous abortion and chromosomal abnormalities in spontaneous abortion tissues.Patients and Methods: The karyotypes of chromosomes of villus tissues from 516 patients with spontaneous abortion who were admitted to our hospital between 2014 and 2019 were analyzed. The data were grouped by the age (ie, ≤ 29, 30– 35, and ≥ 36 years old) in which embryonic chromosomal abnormality rate, and abnormal distribution of chromosome number in abortion tissue of 294 patients.Results: The examination of 516 samples of spontaneous abortion tissues showed that the chromosomes of 294 samples were abnormal, and the abnormality rate was 60.97%. Among 294 samples with chromosomal abnormalities, 136 (89.47%) samples had numerical chromosomal abnormalities, 15 (9.87%) samples had structural chromosomal abnormalities, and 1 (0.07%) sample had uniparental disomy. A total of 308 couples voluntarily underwent peripheral blood chromosome analysis. The results showed that 56 couples had abnormality in one or both spouses, and rate of embryonic chromosomal abnormality in these 26 cases with chromosomal abnormalities was 100%.Conclusion: Numerical and structural chromosomal abnormalities are among the important causes of embryonic arrest, while the increase in age could also be a high-risk factor for embryonic chromosomal abnormality. Therefore, examination of the karyotypes of embryo chorionic villus may help understand the reasons of embryonic arrest, which could provide important guidance for more genetic counseling for patients with early spontaneous abortion, as well as improving the preconception preparation.Keywords: spontaneous abortion, villus tissue, HLPA test, chromosomal abnormalityhttps://www.dovepress.com/clinical-significance-of-application-of-chromosomal-karyotyping-of-vil-peer-reviewed-fulltext-article-IJWHspontaneous abortionvillus tissuehlpa testchromosomal abnormality |
| spellingShingle | Zhang S Wu L Li X Yang L Shen S Shen H Zhao D Qi L Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues International Journal of Women's Health spontaneous abortion villus tissue hlpa test chromosomal abnormality |
| title | Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues |
| title_full | Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues |
| title_fullStr | Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues |
| title_full_unstemmed | Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues |
| title_short | Clinical Significance of Application of Chromosomal Karyotyping of Villus Tissues |
| title_sort | clinical significance of application of chromosomal karyotyping of villus tissues |
| topic | spontaneous abortion villus tissue hlpa test chromosomal abnormality |
| url | https://www.dovepress.com/clinical-significance-of-application-of-chromosomal-karyotyping-of-vil-peer-reviewed-fulltext-article-IJWH |
| work_keys_str_mv | AT zhangs clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT wul clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT lix clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT yangl clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT shens clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT shenh clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT zhaod clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues AT qil clinicalsignificanceofapplicationofchromosomalkaryotypingofvillustissues |