Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i>
Leucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases (RLKs) in plants. They play roles in plant growth and developmental and physiological processes, but less is known about the functions of LRR-RLKs in <i>Medicago truncatula</i>....
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2075-1729/10/9/176 |
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| author | Juan Meng Jie Yang Mengdi Peng Xiaolin Liu Hengbin He |
| author_facet | Juan Meng Jie Yang Mengdi Peng Xiaolin Liu Hengbin He |
| author_sort | Juan Meng |
| collection | DOAJ |
| description | Leucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases (RLKs) in plants. They play roles in plant growth and developmental and physiological processes, but less is known about the functions of LRR-RLKs in <i>Medicago truncatula</i>. Our genome-wide analysis revealed 329 <i>LRR-RLK</i> genes in the <i>M.</i><i>truncatula</i> genome. Phylogenetic and classification analysis suggested that these genes could be classified into 15 groups and 24 subgroups. A total of 321 genes were mapped onto all chromosomes, and 23 tandem duplications (TDs) involving 56 genes were distributed on each chromosome except 4. Twenty-seven <i>M.</i><i>truncatula</i> LRR-RLK segmental duplication gene pairs were colinearly related. The exon/intron organization, motif composition and arrangements were relatively conserved among members of the same groups or subgroups. Using publicly available RNAseq data and quantitative real-time polymerase chain reaction (qRT-PCR), expression profiling suggested that <i>LRR-RLKs</i> were differentially expressed among different tissues, while some were expressed specifically in the roots and nodules. The expression of <i>LRR-RLKs</i> in A17 and 4 nodule mutants under rhizobial infection showed that 36 <i>LRR-RKLs</i> were highly upregulated in the <i>sickle</i> (<i>skl</i>) mutant [an ethylene (ET)-insensitive, Nod factor-hypersensitive mutant] after 12 h of rhizobium inoculation. Among these <i>LRR-RLKs</i>, six genes were also expressed specifically in the roots and nodules, which might be specific to the Nod factor and involved in autoregulation of the nodulation signal. Our results provide information on the <i>LRR-RLK</i> gene family in <i>M. truncatula</i> and serve as a guide for functional research of the <i>LRR-RLKs</i>. |
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| last_indexed | 2024-03-09T06:07:38Z |
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| spelling | doaj.art-c9cd8e9069484e8293b6973bbad5114e2023-12-03T12:01:57ZengMDPI AGLife2075-17292020-09-0110917610.3390/life10090176Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i>Juan Meng0Jie Yang1Mengdi Peng2Xiaolin Liu3Hengbin He4Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaLeucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases (RLKs) in plants. They play roles in plant growth and developmental and physiological processes, but less is known about the functions of LRR-RLKs in <i>Medicago truncatula</i>. Our genome-wide analysis revealed 329 <i>LRR-RLK</i> genes in the <i>M.</i><i>truncatula</i> genome. Phylogenetic and classification analysis suggested that these genes could be classified into 15 groups and 24 subgroups. A total of 321 genes were mapped onto all chromosomes, and 23 tandem duplications (TDs) involving 56 genes were distributed on each chromosome except 4. Twenty-seven <i>M.</i><i>truncatula</i> LRR-RLK segmental duplication gene pairs were colinearly related. The exon/intron organization, motif composition and arrangements were relatively conserved among members of the same groups or subgroups. Using publicly available RNAseq data and quantitative real-time polymerase chain reaction (qRT-PCR), expression profiling suggested that <i>LRR-RLKs</i> were differentially expressed among different tissues, while some were expressed specifically in the roots and nodules. The expression of <i>LRR-RLKs</i> in A17 and 4 nodule mutants under rhizobial infection showed that 36 <i>LRR-RKLs</i> were highly upregulated in the <i>sickle</i> (<i>skl</i>) mutant [an ethylene (ET)-insensitive, Nod factor-hypersensitive mutant] after 12 h of rhizobium inoculation. Among these <i>LRR-RLKs</i>, six genes were also expressed specifically in the roots and nodules, which might be specific to the Nod factor and involved in autoregulation of the nodulation signal. Our results provide information on the <i>LRR-RLK</i> gene family in <i>M. truncatula</i> and serve as a guide for functional research of the <i>LRR-RLKs</i>.https://www.mdpi.com/2075-1729/10/9/176<i>M. truncatula</i>leucine-rich repeat receptor-like kinase (LRR-RLKs)phylogenetic analysisevolutionary analysisexpression profiling |
| spellingShingle | Juan Meng Jie Yang Mengdi Peng Xiaolin Liu Hengbin He Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i> Life <i>M. truncatula</i> leucine-rich repeat receptor-like kinase (LRR-RLKs) phylogenetic analysis evolutionary analysis expression profiling |
| title | Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i> |
| title_full | Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i> |
| title_fullStr | Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i> |
| title_full_unstemmed | Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i> |
| title_short | Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in <i>Medicago truncatula</i> |
| title_sort | genome wide characterization evolution and expression analysis of the leucine rich repeat receptor like protein kinase lrr rlk gene family in i medicago truncatula i |
| topic | <i>M. truncatula</i> leucine-rich repeat receptor-like kinase (LRR-RLKs) phylogenetic analysis evolutionary analysis expression profiling |
| url | https://www.mdpi.com/2075-1729/10/9/176 |
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