Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland
<i>Listeria monocytogenes</i>, an important foodborne pathogen, may be present in different kinds of food and in food processing environments where it can persist for a long time. In this study, 28 <i>L. monocytogenes</i> isolates from fish and fish manufactures were characte...
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/24/9419 |
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| author | Kinga Wieczorek Arkadiusz Bomba Jacek Osek |
| author_facet | Kinga Wieczorek Arkadiusz Bomba Jacek Osek |
| author_sort | Kinga Wieczorek |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i>, an important foodborne pathogen, may be present in different kinds of food and in food processing environments where it can persist for a long time. In this study, 28 <i>L. monocytogenes</i> isolates from fish and fish manufactures were characterized by whole genome sequencing (WGS). Core genome multilocus sequence typing (cgMLST) analysis was applied to compare the present isolates with publicly available genomes of <i>L. monocytogenes</i> strains recovered worldwide from food and from humans with listeriosis. All but one (96.4%) of the examined isolates belonged to molecular serogroup IIa, and one isolate (3.6%) was classified to serogroup IVb. The isolates of group IIa were mainly of MLST sequence types ST121 (13 strains) and ST8 (four strains) whereas the isolate of serogroup IVb was classified to ST1. Strains of serogroup IIa were further subtyped into eight different sublineages with the most numerous being SL121 (13; 48.1% strains) which belonged to six cgMLST types. The majority of strains, irrespective of the genotypic subtype, had the same antimicrobial resistance profile. The cluster analysis identified several molecular clones typical for <i>L. monocytogenes</i> isolated from similar sources in other countries; however, novel molecular cgMLST types not present in the <i>Listeria</i> database were also identified. |
| first_indexed | 2024-03-10T14:11:08Z |
| format | Article |
| id | doaj.art-c9cddb6c50f34cf8850e92fd8c815f1f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:11:08Z |
| publishDate | 2020-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c9cddb6c50f34cf8850e92fd8c815f1f2023-11-21T00:13:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012124941910.3390/ijms21249419Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in PolandKinga Wieczorek0Arkadiusz Bomba1Jacek Osek2Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, PolandDepartment of Omics Analyzes, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, PolandDepartment of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland<i>Listeria monocytogenes</i>, an important foodborne pathogen, may be present in different kinds of food and in food processing environments where it can persist for a long time. In this study, 28 <i>L. monocytogenes</i> isolates from fish and fish manufactures were characterized by whole genome sequencing (WGS). Core genome multilocus sequence typing (cgMLST) analysis was applied to compare the present isolates with publicly available genomes of <i>L. monocytogenes</i> strains recovered worldwide from food and from humans with listeriosis. All but one (96.4%) of the examined isolates belonged to molecular serogroup IIa, and one isolate (3.6%) was classified to serogroup IVb. The isolates of group IIa were mainly of MLST sequence types ST121 (13 strains) and ST8 (four strains) whereas the isolate of serogroup IVb was classified to ST1. Strains of serogroup IIa were further subtyped into eight different sublineages with the most numerous being SL121 (13; 48.1% strains) which belonged to six cgMLST types. The majority of strains, irrespective of the genotypic subtype, had the same antimicrobial resistance profile. The cluster analysis identified several molecular clones typical for <i>L. monocytogenes</i> isolated from similar sources in other countries; however, novel molecular cgMLST types not present in the <i>Listeria</i> database were also identified.https://www.mdpi.com/1422-0067/21/24/9419<i>Listeria</i>fishfish production environmentWGScgMLSTcluster analysis |
| spellingShingle | Kinga Wieczorek Arkadiusz Bomba Jacek Osek Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland International Journal of Molecular Sciences <i>Listeria</i> fish fish production environment WGS cgMLST cluster analysis |
| title | Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland |
| title_full | Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland |
| title_fullStr | Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland |
| title_full_unstemmed | Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland |
| title_short | Whole-Genome Sequencing-Based Characterization of <i>Listeria monocytogenes</i> from Fish and Fish Production Environments in Poland |
| title_sort | whole genome sequencing based characterization of i listeria monocytogenes i from fish and fish production environments in poland |
| topic | <i>Listeria</i> fish fish production environment WGS cgMLST cluster analysis |
| url | https://www.mdpi.com/1422-0067/21/24/9419 |
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