Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province
Enterocytozoon bieneusi is an important pathogen commonly found in humans and animals. Farmed animals with close contact to humans are important hosts of E. bieneusi. The role of goats in the transmission of E. bieneusi, however, remains unclear. In this study, 341 fresh fecal samples of black goats...
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EDP Sciences
2019-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite190115/parasite190115.html |
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| author | Zhou Huan-Huan Zheng Xin-Li Ma Tian-Ming Qi Meng Cao Zong-Xi Chao Zhe Wei Li-Min Liu Quan-Wei Sun Rui-Ping Wang Feng Zhang Yan Lu Gang Zhao Wei |
| author_facet | Zhou Huan-Huan Zheng Xin-Li Ma Tian-Ming Qi Meng Cao Zong-Xi Chao Zhe Wei Li-Min Liu Quan-Wei Sun Rui-Ping Wang Feng Zhang Yan Lu Gang Zhao Wei |
| author_sort | Zhou Huan-Huan |
| collection | DOAJ |
| description | Enterocytozoon bieneusi is an important pathogen commonly found in humans and animals. Farmed animals with close contact to humans are important hosts of E. bieneusi. The role of goats in the transmission of E. bieneusi, however, remains unclear. In this study, 341 fresh fecal samples of black goats were collected from five locations in Hainan Province, China. Enterocytozoon bieneusi was identified and genotyped by sequences of the internal transcribed spacer (ITS) region. Phylogenetic analysis was performed by constructing a neighbor-joining tree of the ITS gene sequences. The average prevalence of E. bieneusi in black goats was 24.0% (82/341) with rates ranging from 6.3% (4/63) to 37.2% (32/86) across the locations (χ2 = 17.252, p < 0.01). Eight genotypes of E. bieneusi were identified, including six known genotypes: CHG5 (n = 47); CHG3 (n = 23); CHG2 (n = 4); CM21 (n = 3); D (n = 2); and AHG1 (n = 1), and two novel genotypes termed HNG-I (n = 1) and HNG-II (n = 1). In the phylogenetic tree, genotype D was clustered into Group 1 and the other identified genotypes were included in Group 2. This represents the first report identifying E. bieneusi in black goats from Hainan Province, with a high prevalence and wide occurrence demonstrated. The two new genotypes identified provide additional insights into the genotypic variations in E. bieneusi. Due to the small percentage of zoonotic genotypes in these animals, there is minimal risk of zoonotic transmission of E. bieneusi. |
| first_indexed | 2024-03-09T09:11:19Z |
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| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T09:11:19Z |
| publishDate | 2019-01-01 |
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| record_format | Article |
| series | Parasite |
| spelling | doaj.art-c9d0a65add874649bf0ac8fea04665672023-12-02T08:43:32ZengEDP SciencesParasite1776-10422019-01-01266210.1051/parasite/2019064parasite190115Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan ProvinceZhou Huan-HuanZheng Xin-LiMa Tian-MingQi MengCao Zong-XiChao ZheWei Li-MinLiu Quan-WeiSun Rui-PingWang FengZhang YanLu GangZhao WeiEnterocytozoon bieneusi is an important pathogen commonly found in humans and animals. Farmed animals with close contact to humans are important hosts of E. bieneusi. The role of goats in the transmission of E. bieneusi, however, remains unclear. In this study, 341 fresh fecal samples of black goats were collected from five locations in Hainan Province, China. Enterocytozoon bieneusi was identified and genotyped by sequences of the internal transcribed spacer (ITS) region. Phylogenetic analysis was performed by constructing a neighbor-joining tree of the ITS gene sequences. The average prevalence of E. bieneusi in black goats was 24.0% (82/341) with rates ranging from 6.3% (4/63) to 37.2% (32/86) across the locations (χ2 = 17.252, p < 0.01). Eight genotypes of E. bieneusi were identified, including six known genotypes: CHG5 (n = 47); CHG3 (n = 23); CHG2 (n = 4); CM21 (n = 3); D (n = 2); and AHG1 (n = 1), and two novel genotypes termed HNG-I (n = 1) and HNG-II (n = 1). In the phylogenetic tree, genotype D was clustered into Group 1 and the other identified genotypes were included in Group 2. This represents the first report identifying E. bieneusi in black goats from Hainan Province, with a high prevalence and wide occurrence demonstrated. The two new genotypes identified provide additional insights into the genotypic variations in E. bieneusi. Due to the small percentage of zoonotic genotypes in these animals, there is minimal risk of zoonotic transmission of E. bieneusi.https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite190115/parasite190115.htmlenterocytozoon bieneusigenotypeits regiongoatschina |
| spellingShingle | Zhou Huan-Huan Zheng Xin-Li Ma Tian-Ming Qi Meng Cao Zong-Xi Chao Zhe Wei Li-Min Liu Quan-Wei Sun Rui-Ping Wang Feng Zhang Yan Lu Gang Zhao Wei Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province Parasite enterocytozoon bieneusi genotype its region goats china |
| title | Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province |
| title_full | Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province |
| title_fullStr | Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province |
| title_full_unstemmed | Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province |
| title_short | Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province |
| title_sort | genotype identification and phylogenetic analysis of enterocytozoon bieneusi in farmed black goats capra hircus from china s hainan province |
| topic | enterocytozoon bieneusi genotype its region goats china |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite190115/parasite190115.html |
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