Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer
Endometrial Carcinoma (EC) is one of the most common cancers of females’ reproductive system. It has been given first rank in the tumors of obstetrics and gynaecology and day by day the incidence of EC gradually increased in recent years, which has critically harmed the woman health. 100 cases of En...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Journal of King Saud University: Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364719317938 |
| _version_ | 1828428399074344960 |
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| author | Wei Zheng Jianying Yang Yuhong Wang Xinru Liu |
| author_facet | Wei Zheng Jianying Yang Yuhong Wang Xinru Liu |
| author_sort | Wei Zheng |
| collection | DOAJ |
| description | Endometrial Carcinoma (EC) is one of the most common cancers of females’ reproductive system. It has been given first rank in the tumors of obstetrics and gynaecology and day by day the incidence of EC gradually increased in recent years, which has critically harmed the woman health. 100 cases of Endometrial cancer and equal number of female healthy subjects were included and serum were separated from blood sample collected in plain vials from all study participant. Total RNA was extracted from exosomes extracted from serum and cDNA were synthesised from 100 ng of total RNA. cDNA were subjected to quantitative real time PCR to study the microRNA-95 and micro-RNA205 expression among cased and controls using Taqman assay. 3.78 mean fold increased exosomalmicroRNA-95 expression was observed while 0.07 mean fold decreased exosomal microRNA was found in endometrial cancer patients. Increased expression of exosomal miRNA-93 expression were linked with smoking (p = 0.002), grade of tumor (p = 0.01), FIGO stage (p < 0.0001) and distance organ metastases (p < 0.0001). Decreased miRNA-205 (0.07 mean fold) expression of exosomal miRNA-205 was observed in EC patients compared to healthy controls and were found to associated with smoking (p = 0.01), lymph node involvement (p = 0.001), FIGO stage (p = 0.004). Poor overall median survival was observed to be associated with increase exosomalmicroRNA-95 and decreased microRNA-205 of endometrial cancer patients. ROC curves showed exosomal miRNA-93 at cut-off value of 3.25 fold increased in serum based exosomal microRNA-93 expression, sensitivity and specificity were 93% and 97%, respectively (AUC was 0.99, p < 0.0001). A negative correlation was observed between exosomal microRNA-93 and exosomal microRNA-205 expression (r = −0.20, p = 0.04).Study concluded that exosomalmicroRNA-95 and exosomal microRNA-205 was associated with worseness of disease and could be a prognostic indicator for endometrial cancer patients. Keywords: miRNA-93, miRNA-205, Expression, Cancer patients, ROC |
| first_indexed | 2024-12-10T17:17:04Z |
| format | Article |
| id | doaj.art-c9d0cc5b08c444adbac37d2ab7ddae69 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-10T17:17:04Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-c9d0cc5b08c444adbac37d2ab7ddae692022-12-22T01:40:05ZengElsevierJournal of King Saud University: Science1018-36472020-01-0132111111115Exosomal miRNA-93 and miRNA-205 expression in endometrial cancerWei Zheng0Jianying Yang1Yuhong Wang2Xinru Liu3Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, Sichuan 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan 610041, ChinaDepartment of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, Sichuan 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan 610041, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, ChinaDepartment of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, Sichuan 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan 610041, China; Corresponding author at: Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, Sichuan 610041, China.Endometrial Carcinoma (EC) is one of the most common cancers of females’ reproductive system. It has been given first rank in the tumors of obstetrics and gynaecology and day by day the incidence of EC gradually increased in recent years, which has critically harmed the woman health. 100 cases of Endometrial cancer and equal number of female healthy subjects were included and serum were separated from blood sample collected in plain vials from all study participant. Total RNA was extracted from exosomes extracted from serum and cDNA were synthesised from 100 ng of total RNA. cDNA were subjected to quantitative real time PCR to study the microRNA-95 and micro-RNA205 expression among cased and controls using Taqman assay. 3.78 mean fold increased exosomalmicroRNA-95 expression was observed while 0.07 mean fold decreased exosomal microRNA was found in endometrial cancer patients. Increased expression of exosomal miRNA-93 expression were linked with smoking (p = 0.002), grade of tumor (p = 0.01), FIGO stage (p < 0.0001) and distance organ metastases (p < 0.0001). Decreased miRNA-205 (0.07 mean fold) expression of exosomal miRNA-205 was observed in EC patients compared to healthy controls and were found to associated with smoking (p = 0.01), lymph node involvement (p = 0.001), FIGO stage (p = 0.004). Poor overall median survival was observed to be associated with increase exosomalmicroRNA-95 and decreased microRNA-205 of endometrial cancer patients. ROC curves showed exosomal miRNA-93 at cut-off value of 3.25 fold increased in serum based exosomal microRNA-93 expression, sensitivity and specificity were 93% and 97%, respectively (AUC was 0.99, p < 0.0001). A negative correlation was observed between exosomal microRNA-93 and exosomal microRNA-205 expression (r = −0.20, p = 0.04).Study concluded that exosomalmicroRNA-95 and exosomal microRNA-205 was associated with worseness of disease and could be a prognostic indicator for endometrial cancer patients. Keywords: miRNA-93, miRNA-205, Expression, Cancer patients, ROChttp://www.sciencedirect.com/science/article/pii/S1018364719317938 |
| spellingShingle | Wei Zheng Jianying Yang Yuhong Wang Xinru Liu Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer Journal of King Saud University: Science |
| title | Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer |
| title_full | Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer |
| title_fullStr | Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer |
| title_full_unstemmed | Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer |
| title_short | Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer |
| title_sort | exosomal mirna 93 and mirna 205 expression in endometrial cancer |
| url | http://www.sciencedirect.com/science/article/pii/S1018364719317938 |
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